CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression

Xue, Yibo; Ding, Meng‐Qi; Xue, Lijun

doi:10.1111/1759-7714.13131

Cited by 47 publications

(34 citation statements)

References 27 publications

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“…Several circRNAs have been reported to be aberrantly expressed and associated with the tumorigenesis of lung cancer . Furthermore, emerging evidence suggests that several circRNAs might contribute to the progression of NSCLC . Accruing evidence demonstrates that circRNA antisense to cerebellar degeneration‐related protein 1 transcript (circCDR1as) could promote the development of hepatocellular carcinoma, colorectal cancer and laryngeal squamous cell carcinoma and inhibit the progression of bladder cancer and ovarian cancer .…”

Section: Introductionmentioning

confidence: 99%

“…7 Furthermore, emerging evidence suggests that several circRNAs might contribute to the progression of NSCLC. [8][9][10] Accruing evidence demonstrates that circRNA antisense to cerebellar degeneration-related protein 1 transcript (circCDR1as) could promote the development of hepatocellular carcinoma, colorectal cancer and laryngeal squamous cell carcinoma and inhibit the progression of bladder cancer and ovarian cancer. [11][12][13][14][15] Importantly, a previous study reveals that circCDR1as acts as an oncogene to facilitate the development of NSCLC.…”

Section: Introductionmentioning

confidence: 99%

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CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

Zhang

Pan

et al. 2020

Thoracic Cancer

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Background Circular RNAs (circRNAs) participate in the development of human cancers by regulating multiple cell processes. CircRNA antisense to the cerebellar degeneration‐related protein 1 transcript (circCDR1as) expression is dysregulated in many cancers, including non‐small‐cell lung cancer (NSCLC). However, the mechanism by which circCDR1as mediates the development of NSCLC remains unknown. Methods A total of 30 paired cancer and normal tissues were collected from patients with NSCLC. The expression levels of circCDR1as, microRNA (miR)‐219a‐5p and Sex determining region Y‐box protein 5 (SOX5) were measured in tissues or cells by quantitative real‐time polymerase chain reaction or western blot. Cell viability, apoptosis, migration and invasion were detected by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐tetrazolium bromide, colony formation, flow cytometry and transwell assays, respectively. The target relationship between miR‐219a‐5p and circCDR1as or SOX5 was validated by dual‐luciferase reporter assay. Results CircCDR1as expression was elevated in NSCLC tissues and cells in comparison to the matched controls. Interference of circCDR1as led to obvious inhibition of cell viability, migration and invasion and increase of apoptosis in NSCLC cells. MiR‐219a‐5p acted as a target of circCDR1as and miR‐219a‐5p downregulation attenuated the regulatory effect of circCDR1as silencing on NSCLC progression. Moreover, miR‐219a‐5p targeted SOX5 to repress the progression of NSCLC in vitro. Besides, circCDR1as knockdown reduced the expression of SOX5 by increasing miR‐219a‐5p level. Conclusion Knockdown of circCDR1as inhibited the progression of NSCLC by decreasing cell viability, migration and invasion and increasing apoptosis by upregulating miR‐219a‐5p and downregulating SOX5.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

Zhang

Pan

et al. 2020

Thoracic Cancer

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“…Meanwhile, circRNA-miRNA-mRNA axis might play an important role in cancer-related pathways (25). Huang et al found that circRNA_001946 could promote EMT and metastasis of NSCLC cells via a circAGFG1/miR-203/ZNF281 axis (14).…”

Section: Discussionmentioning

confidence: 99%

“…Previous study indicated that circRNAs regulated the expression of the target genes via acting as a microRNA sponge (13). Xue et al found that circAGFG1 promoted metastasis in NSCLC cells by sponging miR-203 (14).…”

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0014130 Inhibits Apoptosis in Non–Small Cell Lung Cancer by Sponging miR-136-5p and Upregulating BCL2

Geng

Bao

Zhang

et al. 2020

Molecular Cancer Research

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Previous studies indicated that circular RNAs (circRNAs) played vital roles in the development of non-small-cell lung cancer (NSCLC). Although hsa_circ_0014130 might be a potential NSCLC biomarker, its function in NSCLC remains unknown. Thus, this study aimed to investigate the role of hsa_circ_0014130 in the progression of NSCLC. The levels of hsa_circ_0014130 in NSCLC tissues and adjacent normal tissues were determined by RT-qPCR. In addition, the expressions of Bcl-2, cleaved caspase 3 in A549 cells were detected with western blot. Meanwhile, the dual luciferase reporter system assay was used to determine the interaction of hsa_circ_0014130 and miR-136-5p, or Bcl-2 and miR-136-5p in NSCLC respectively. The level of hsa_circ_0014130 was significantly upregulated in NSCLC tissues. Downregulation of hsa_circ_0014130 markedly inhibited the proliferation and invasion of A549 cells via inducing apoptosis. In addition, downregulation of hsa_circ_0014130 inhibited the tumorigenesis of subcutaneous A549 xenograft in mice in vivo. Meanwhile, mechanistic analysis indicated that downregulation of hsa_circ_0014130 decreased the expression of miR-136-5p targeted gene Bcl-2 via acting as a competitive 'sponge' of miR-136-5p. In this study, we found that hsa_circ_0014130 was upregulated in NSCLC tissues. In addition, hsa_circ_0014130 functions as a tumor promoter in NSCLC to promote tumor growth through up-regulating Bcl-2 partially via 'sponging' miR-136-5p. Implications statementIn conclusion, hsa_circ_0014130 might function as a prognostic factor for patients with NSCLC and might be a therapeutic target for the treatment of NSCLC in future.

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“…[20] It has been shown that circRNA can affect the migration, invasion and EMT in various tumors, including BC. [21][22][23][24][25] Dual luciferase reporter and RIP assay results showed that circPVT1 could sponge miR-140-3p, and that miR-140-3p could target TRPS1. Further, Cytological studies showed that the lower circPVT1 expression can effectively inhibit cell proliferation, migration, invasion, and the EMT in BC cells by targeting TRPS1 via miR-140-3p.…”

Section: Discussionmentioning

confidence: 99%

CircPVT1 Promotes Bladder Cancer Progression by Acting as a ceRNA for miR-140-3p to Target TRPS1

Chi¹,

Sun²,

Zhao³

et al. 2020

Preprint

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Background: Bladder cancer (BC) is one of the most malignancy tumor in the urinary system. Therefore, further studies are needed to revealed the molecular mechanism of BC progression and development. Previous study demonstrated that the deregulation of circRNAs can regulate cell biological functions in tumorigenesis and development. However, the roles of circPVT1 in BC have not yet been revealed. Materials and methods: The expression level of circPVT1, miR-140-3p, and TRPS1 were measured by RT-PCR in BC tissues and cells; dual luciferase reporter and RIP assay showed that circRNA served as a sponge for miRNA, and miRNA could target mRNA. In vitro, effects of overexpression circPVT1 or sh-circPVT1 can regulate BC cells’ proliferation, migration, invasion were detected by CCK-8 assay, wound healing assay, and transwell assay. Results: Our research demonstrated that the expression of circPVT1 was upregulated in BC tissues and cell lines, and increased in metastatic tissues compared to that of non-metastatic tissues. CircPVT1 sponging miR-140-3p to target TRPS1 was revealed by dual luciferase reporter and RIP assay. In addition, the expression level of miR-140-3p was reduced in BC tumor tissues, and TRPS1 was significantly increased in BC tumor tissues. Pearson correlation analysis showed that miR-140-3p with circPVT1 and TRPS1 as compare with miR-140-3p have a moderately negative correlation, and there was a moderately positive correlation between circPVT1 with TRPS1. Further, cytological studies found that circPVT1 enhance BC cells’ proliferation, migration, and invasion by targeting TRPS1 via miR-140-3p. Conclusion: CircPVT1 plays a tumor enhancement role in BC and that can effectively promote cell proliferation, migration, invasion and EMT by targeting the miR-140-3p/TRPS1 axis. CircPVT1 may be a novel potential treatment and diagnosis biomarker in BC.

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CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression

Cited by 47 publications

References 27 publications

CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

Circular RNA hsa_circ_0014130 Inhibits Apoptosis in Non–Small Cell Lung Cancer by Sponging miR-136-5p and Upregulating BCL2

CircPVT1 Promotes Bladder Cancer Progression by Acting as a ceRNA for miR-140-3p to Target TRPS1

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CircAGFG1 sponges miR‐203 to promote EMT and metastasis of non‐small‐cell lung cancer by upregulating ZNF281 expression

Cited by 47 publications

References 27 publications

CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

CircRNA CDR1as knockdown inhibits progression of non‐small‐cell lung cancer by regulating miR‐219a‐5p/SOX5 axis

Circular RNA hsa_circ_0014130 Inhibits Apoptosis in Non–Small Cell Lung Cancer by Sponging miR-136-5p and Upregulating BCL2

CircPVT1 Promotes Bladder Cancer&nbsp;Progression by Acting as a ceRNA for miR-140-3p&nbsp;to Target TRPS1

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CircPVT1 Promotes Bladder Cancer Progression by Acting as a ceRNA for miR-140-3p to Target TRPS1