CircRNA hsa_circRNA_101996 increases cervical cancer proliferation and invasion through activating TPX2 expression by restraining miR‐8075

Song, Tiefang; Xu, Aili; Zhang, Zongfeng; Gao, Fei; Zhao, Lin; Chen, Xiuhui; Gao, Jiayin; Kong, Xiangdong

doi:10.1002/jcp.28128

Cited by 168 publications

(121 citation statements)

References 33 publications

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“…20 Moreover, results from Xianchao Kong el al showed that circ101996 might act as an oncogene of CC through increasing the expression of TPX2 through miR-8075. 21 In this study, a circRNA microarray analysis was conducted on two CC-associated circRNA GSE datasets (GSE102686 and GSE113696) downloaded from the GEO database (www.ncbi.nlm.nih.gov/geo) to screen specific circRNAs that might involve in the pathogenesis of CC. Among the different expressed circRNAs, circ_103973 was identified to be significantly increased.…”

Section: Discussionmentioning

confidence: 99%

<p>Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression</p>

Zhu

Jiang

Zhang

et al. 2020

OTT

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Background: Cervical cancer (CC) ranks as the second most common malignancy in women, accounting for more two 2 million deaths every year in the world. Recently, circular RNAs (circRNAs) have been reported to regulate the progression of multiple human tumors; however, whether it involves in CC remains largely elusive. Materials and Methods: Two GEO circRNA expression profiles (GSE102686, GSE113696) were downloaded to analyze the differentially expressed circRNAs using bioinformatics methods. Expression of circ_103973, miR-335 and PPP6C in CC tissues and cell lines were examined by qRT-PCR. Cell apoptosis was assessed with PI/Annexin-V double staining followed by the analysis of flow cytometry. Cell proliferation was evaluated by MTT and colony formation assays. Interaction between circ_103973 and miR-335, as well as miR-335 and PPP6C, were verified by dual-luciferase reporter assay. Results: Circ_103973 was found to be highly expressed in both GSE102686 and GSE113696 datasets as well as in CC tissue samples and cell lines. Higher levels of circ_103973 were correlated to a worse outcome of CC patients. Knockdown of circ_103973 significantly promoted CC cell apoptosis and inhibited CC cell proliferation in vitro. Mechanistically, we demonstrated that circ_103973 served as a sponge of miR-335, which directly targeted PPP6C in CC cells. miR-335 was found to be decreased in CC, while PPP6C was found to be increased in CC. Moreover, anti-miR-335 could reverse the inhibitory effects of circ_103973 knockdown on CC cell proliferation, and this phenomenon could be blocked by si-PPP6C. Conclusion: Circ_103973 promoted CC cell proliferation in vitro by physically binding miR-335, which further targeted and regulated PPP6C.

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Section: Discussionmentioning

confidence: 99%

<p>Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression</p>

Zhu

Jiang

Zhang

et al. 2020

OTT

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“…Many studies have reported the up-regulation and miRNA-sponging roles of circRNAs in CC. For instance, circ_101996 was overexpressed in cervical cancerous tissues [44]. One study showed that circ_0084927 was signi cantly up-regulated in malignant pleural effusion (MPE) of lung cancer [45].…”

Section: Discussionmentioning

confidence: 99%

circ_0084927 promotes cervical carcinogenesis by sponging miR-1179 that suppresses CDK2, a cell cycle-related gene

Zhu

Song

et al. 2020

Preprint

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Abstract Background: Cervical cancer (CC) is a highly malignant tumor. found in the lowermost part of the womb. Evolving researchesstudies on CC have unveiled a conceptreported that circRNA exerts important rolesplays a crucial role in CC progression. In this study, we mainly exploredinvestigated the rolemain function of a novel circRNA, circ_0084927, and its regulatory network in theCC development of CC. Methods: qRT-PCR was applied to evaluate the expression of circ_0084927, miR-1179, and CDK2 mRNA in CC tissues and cells. Dual-luciferase reporting experiments and RNA immunoprecipitation (RIP) assay were conducted to validate the target relationship of miR-1179 with circ_0084927 and CDK2 mRNA. CCK-8 and BrdU assay wasassays were also used to evaluate CC cell proliferation. The adhesion and apoptosis phenotypes of CC cells were measured byusing cell-matrix adhesion and caspase 3 activation assay. Flow cytometry was also employed to detect the CC cell cycle. Results: Our results indicated that circ_0084927 was up-regulated in CC tissues and cells, and. Findings also revealed that circ_0084927 silence inhibited CC cell proliferation and adhesion, while facilitating apoptosis as well asand triggering cell cycle arrest. On the other handHowever, miR-1179 down-regulation appeared in CC tissues. Additionally,Apart from observing that circ_0084927 abolished miR-1179’s inhibitory effects on cell proliferation and adhesion. Our study showed, it was found that CDK2 was up-regulated in CC tissues and played awas instrumental in cancer-promoting role. Furthermore, promotion. Also observed was that miR-1179 directly targeted CDK2, thereby inhibiting CDK2’s promotion on the malignant phenotypes of CC cells. Lastly, results indicated that circ_0084927 revoked the inhibitory effect of miR-1179 on CDK2 by sponging miR-1179. Conclusion: Circcirc_0084927 promoted cervical carcinogenesis by sequestering miR-1179 that, which directly targeted CDK2. Our results shed light onalso provided novel candidate targets for CC treatment in that it revealed the circ_0084927/miR-1179/CDK2 regulatory network that strengthened CC aggressiveness, providing novel candidate targets for CC treatment..

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“…Despite intense screening and various treatment options such as surgery, radiotherapy and chemotherapy, prognosis in CESC remains poor due to high risk of metastasis and recurrence. Additionally, mounting evidence suggests that altered expression of many genes contributes to CESC pathogenesis [15][16][17]. Therefore, we expect to identify some sensitive and novel biomarkers for diagnosis and prognosis in CESC.…”

Section: Discussionmentioning

confidence: 99%

Identification of hub genes in cervical cancer using weighted gene co-expression network analysis

Gong

Han

et al. 2020

Preprint

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Background Cervical cancer ranks second among malignancies in females around the world. Due to the elevated incidence and mortality of this malignancy, deciphering its pathogenesis and identifying related biomarkers is urgently required. Methods First, raw cervical squamous cell carcinoma (CESC) data in GSE63514 were downloaded from the Gene Expression Omnibus (GEO) database. Then, weighted Correlation Network Analysis (WGCNA) was performed to build a co-expression network. Next, comprehensive bioinformatics was performed to determine hub genes, and assess the associated functional annotation, prognostic signature, tumor immunity, DNA mismatch repair, methylation mechanism, candidate molecular drugs, and gene mutations. Results From the key module, ALOX12B, KRT78, RHOD and ZNF750 were selected for validation. K-M plots indicated that these genes had good diagnostic and prognostic values in CESC. Moreover, mutations in these hub genes resulted in the downregulation of most immune genes in CESC. On the other hand, most of the four core genes were negatively correlated with DNA mismatch genes. In addition, we found that RHOD and ZNF750 had decreased methylation in the disease state, while ALOX12B and KRT78 showed no significant differences. Meanwhile, GSVA revealed that most core genes had associations with P53 signaling and the hypoxia signaling pathway. Conclusion WGCNA could identify groups of genes significantly associated with cervical cancer prognosis. These findings provide new insights into CESC pathogenesis, and identify ALOX12B, KRT78, RHOD and ZNF750 as candidate biomarkers for CESC diagnosis and prognosis.

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CircRNA hsa_circRNA_101996 increases cervical cancer proliferation and invasion through activating TPX2 expression by restraining miR‐8075

Cited by 168 publications

References 33 publications

<p>Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression</p>

<p>Hsa_circ_103973 Acts as a Sponge of miR-335 to Promote Cervical Cancer Progression</p>

circ_0084927 promotes cervical carcinogenesis by sponging miR-1179 that suppresses CDK2, a cell cycle-related gene

Identification of hub genes in cervical cancer using weighted gene co-expression network analysis

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