circRNA-miRNA-mRNA network analysis to explore the pathogenesis of abnormal spermatogenesis due to aberrant m6A methylation

Zhong, Dandan; Zhang, Liyin; Huang, Kongwei; Chen, Mengjie; Chen, Yaling; Liu, Qingyou; Shi, Deshun; Li, Hui

doi:10.1007/s00441-022-03725-7

Cited by 8 publications

(2 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to investigate the potential regulatory mechanism in breast cancer, the KIF2C-related ceRNA network was predicted through Miranda 3.3a software [ 46 ]. The potential miRNAs targeting KIF2C were firstly predicted with the cut-off of binding score > 500 and free energy score < -500.…”

Section: Methodsmentioning

confidence: 99%

KIF2C is a prognostic biomarker associated with immune cell infiltration in breast cancer

Liu

Xue

et al. 2023

BMC Cancer

View full text Add to dashboard Cite

Background The kinesin-13 family member 2C (KIF2C) is a versatile protein participating in many biological processes. KIF2C is frequently up-regulated in multiple types of cancer and is associated with cancer development. However, the role of KIF2C in immune cell infiltration of tumor microenvironment and immunotherapy in breast cancer remains unclear. Methods The expression of KIF2C was analyzed using Tumor Immune Estimation Resource (TIMER) database and further verified by immunohistochemical staining in human breast cancer tissues. The correlation between KIF2C expression and clinical parameters, the impact of KIF2C on clinical prognosis and independent prognostic factors were analyzed by using TCGA database, the Kaplan-Meier plotter, and Univariate and multivariate Cox analyses, respectively. The nomograms were constructed according to independent prognostic factors and validated with C-index, calibration curves, ROC curves, and decision curve analysis. A gene set enrichment analysis (GSEA) was performed to explore the underlying molecular mechanisms of KIF2C. The degree of immune infiltration was assessed by the Estimation of Stromal and Immune cells in Malignant Tumor tissues using the Expression (ESTIMATE) algorithm and the single sample GSEA (ssGSEA). The Tumor mutational burden and Tumor Immune Dysfunction and Rejection (TIDE) were used to analyze immunotherapeutic efficiency. Finally, the KIF2C-related competing endogenous RNA (ceRNA) network was constructed to predict the putative regulatory mechanisms of KIF2C. Results KIF2C was remarkably up-regulated in 18 different types of cancers, including breast cancer. Kaplan-Meier survival analysis showed that high KIF2C expression was associated with poor overall survival (OS). KIF2C expression was associated with clinical parameters such as age, TMN stage, T status, and molecular subtypes. We identified age, stage, estrogen receptor (ER) and KIF2C expression as OS-related independent prognosis factors for breast cancer. An OS-related nomogram was developed based on these independent prognosis factors and displayed good predicting ability for OS of breast cancer patients. Finally, our results revealed that KIF2C was significantly related to immune cell infiltration, tumor mutational burden, and immunotherapy in patients with breast cancer. Conclusion KIF2C was overexpressed in breast cancer and was positively correlated with immune cell infiltration and immunotherapy response. Therefore, KIF2C can serve as a potential biomarker for prognosis and immunotherapy in breast cancer.

show abstract

Section: Methodsmentioning

confidence: 99%

KIF2C is a prognostic biomarker associated with immune cell infiltration in breast cancer

Liu

Xue

et al. 2023

BMC Cancer

View full text Add to dashboard Cite

show abstract

“…The CAMSAP1 gene, located at 9q34.3, contains a total of 19 exons and was widely expressed in tissues and organs of the human reproductive system. Its expression levels in reproductive system tissues, from high to low, are as follows: testicles, endometrium, ovaries, and prostate, indicating that it was related to the function of the human reproductive system, especially the male reproductive system [25]. The biological function of circular RNA can provide potential targets for the clinical diagnosis and treatment of male infertility.…”

Section: Circular Rna Participated In Male Infertilitymentioning

confidence: 99%

Circular RNA regulates male spermatogenesis: a narrative review

Peng,

2024

Middle East Fertil Soc J

View full text Add to dashboard Cite

Spermatogenesis was crucial for adult male animals to achieve reproductive function, and this complex physiological process required timely and moderate expression of related genes. A large number of epigenetic regulatory factors were involved, including cyclic RNA. Circular RNA had various characteristics such as rich expression, evolutionary conservation, cell or tissue specificity, and higher resistance to exonuclease or ribonuclease degradation. It can regulate the expression of parental genes and function as mRNA traps, miRNAs, or proteins in the corpus cavernous; it can also participate in the process of spermatogenesis through RNA-binding proteins, including the formation of reproductive stem cells, sperm formation, seminal plasma composition, and testicular tissue formation.

show abstract

Comprehensive analysis of differentially expressed mRNAs, circRNAs, and miRNAs and their ceRNA network in the testis of cattle–yak, yak, and cattle

Cao,

Xiong,

Wang

et al. 2024

Genomics

View full text Add to dashboard Cite

circRNA-miRNA-mRNA network analysis to explore the pathogenesis of abnormal spermatogenesis due to aberrant m6A methylation

Cited by 8 publications

References 52 publications

KIF2C is a prognostic biomarker associated with immune cell infiltration in breast cancer

KIF2C is a prognostic biomarker associated with immune cell infiltration in breast cancer

Circular RNA regulates male spermatogenesis: a narrative review

Comprehensive analysis of differentially expressed mRNAs, circRNAs, and miRNAs and their ceRNA network in the testis of cattle–yak, yak, and cattle

Contact Info

Product

Resources

About