CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway

Liu, Junqi; Xue, Nannan; Guo, Yuexin; Niu, Kerun; Gao, Liang; Zhang, Song; Gu, Hongtao; Wang, Xin; Zhao, Di; Fan, Ruitai

doi:10.18632/aging.102580

Cited by 128 publications

(97 citation statements)

References 33 publications

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“…CircRNA_100367 was upregulated, and this dysregulation induced the suppression in radiation sensitivity in ESCC cells. 24 Has_circRNA_006660 was upregulated and sponged miR-1276 to increase the radiation sensitivity in nasopharyngeal carcinoma. 25 The effect of circRNAs dysregulation on the radiation sensitivity of cancers remained disunited; however, the upregulation of circ_0062020 further contributed to the facilitation of radiation sensitivity in PCa.…”

Section: Discussionmentioning

confidence: 99%

<p>Circ_0062020 Knockdown Strengthens the Radiosensitivity of Prostate Cancer Cells</p>

Zhi

et al. 2020

CMAR

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Section: Discussionmentioning

confidence: 99%

<p>Circ_0062020 Knockdown Strengthens the Radiosensitivity of Prostate Cancer Cells</p>

Zhi

et al. 2020

CMAR

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“…RT is one of the primary therapeutic modalities in patients with ESCC. The existence of radioresistance is a major limit to achieve long-term survival in ESCC, which has been linked to an increased likelihood of recurrence and distant metastasis [25][26][27].…”

Section: Discussionmentioning

confidence: 99%

NRAGE, a potential biomarker, induces radioresistance in two- and three-dimensional culture and confers poor prognosis in esophageal squamous cell carcinoma

Hong-bin¹,

Wang²,

Xiao³

et al. 2020

Preprint

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Background To explore the mechanism of NRAGE enhancing radioresistance of ESCC in 2D and 3D levels. Methods Stably NRAGE-overexpressed ESCC cells and 3D-printing models for ESCC cells were established. Then, cellular malignancy indexes, such as cell morphology, proliferation, radioresistance, motility, apoptosis, cell cycle, and proteins of the Wnt/β-catenin pathway, were compared between radioresistant and its parental cells in 2D and 3D levels. Additionally, 44 paraffin ESCC specimens with radical radiotherapy were selected to examine NRAGE and β-catenin protein expression and analyze the clinical correlation. Results Experiments in 2D culture showed that Eca109/NRAGE cells’ morphology was more irregular, elongated spindle-shaped and disappeared polarity. It obtained faster growth ability, stronger resistance to irradiation, enhanced motility, reduced apoptosis ratio and cell cycle rearrangement. Moreover, Western blot results showed β-catenin, p-Gsk-3β and CyclinD1 expressions were induced, while p-β-catenin and Gsk-3β expressions decreased in Eca109/NRAGE cells. Experiments in the 3D-printing model showed Eca109/NRAGE cell-laden 3D scaffolds had the advantage on growth and spheroiding according to the brigbtfield observation, scanning electron microscopy and Ki-67 IHC staining, and higher expression at the β-catenin protein. Clinical analysis showed that NRAGE expression was higher in tumor tissues than in control tissues of ESCC patients from the Public DataBase. Compared with radiotherapy effective group, both NRAGE total and nuclear and β-catenin nuclear expressions were significantly upregulated from ESCC specimens in invalid group. Further analysis showed a positive and linear correlation between NRAGE nuclear and β-catenin nuclear expressions. Additionally, results from univariate and multivariate analyses revealed NRAGE nuclear expression could serve as a risk factor for ESCC patients receiving radical radiotherapy. Conclusion ESCC cells with NRAGE nuclear accumulation demonstrated greater radioresistance, which may be related to the activation of the Wnt/β-catenin signaling pathway. It indicated that NRAGE nuclear expression was a potential biomarker for monitoring radiotherapeutic response.

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“…The level of circRNA_100367 is increased in radioresistant ESCC cells. It binds to miR-217 to promote Wnt3mediated EMT, resulting in reduced sensitivity of cancer cells to radiation [105]. CircVRK1 is decreased in ESCC tissues, and patients with low level of circVRK1 have a poor prognosis.…”

Section: Esophageal Cancermentioning

confidence: 99%

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

et al. 2020

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Circular RNAs (circRNAs), one type of non-coding RNA, were initially misinterpreted as nonfunctional products of pre-mRNA mis-splicing. Currently, circRNAs have been proven to manipulate the functions of diverse molecules, including non-coding RNAs, mRNAs, DNAs and proteins, to regulate cell activities in physiology and pathology. Accumulating evidence indicates that circRNAs play critical roles in tumor genesis, development, and sensitivity to radiation and chemotherapy. Radiotherapy and chemotherapy are two primary types of intervention for most cancers, but their therapeutic efficacies are usually retarded by intrinsic and acquired resistance. Thus, it is urgent to develop new strategies to improve therapeutic responses. To achieve this, clarification of the underlying mechanisms affecting therapeutic responses in cancer is needed. This review summarizes recent progress and mechanisms of circRNAs in cancer resistance to radiation and chemotherapy, and it discusses the limitations of available knowledge and potential future directions.

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CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway

Cited by 128 publications

References 33 publications

<p>Circ_0062020 Knockdown Strengthens the Radiosensitivity of Prostate Cancer Cells</p>

<p>Circ_0062020 Knockdown Strengthens the Radiosensitivity of Prostate Cancer Cells</p>

NRAGE, a potential biomarker, induces radioresistance in two- and three-dimensional culture and confers poor prognosis in esophageal squamous cell carcinoma

Functions and mechanisms of circular RNAs in cancer radiotherapy and chemotherapy resistance

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