circSLC8A1 Acts as a Tumor Suppressor in Prostate Cancer via Sponging miR-21

Abstract

Background. There is more and more evidence showed that circRNAs played essentially role in the regulation of various biological processes. The role of circSLC8A1 in prostate cancer (PCa) is yet little known. Methods. The CircSLC8A1 expression in human prostate cancer was measured by qRT-PCR. The interplay between the specific circRNA, miRNA, and mRNA was investigated by RT-PCR and luciferase reporter assay. Through transient transfection of siRNA, the impacts of circSLC8A1 on PCa were discussed. Cell cycle evaluation, transwell assay, and CCK-8 assay were employed to determine its biological influences. Results. In this study, our data revealed that circSLC8A1 was downregulated in PCa tissues and cells. The reduction of circSLC8A1 resulted in the inhibition of cell proliferation and migration. In mechanism, circSLC8A1 exhibited a direct interaction with miR-21 and displayed as a miRNA sponge to inhibit PCa progression. The functional analysis revealed that the circSLC8A1/miR-21 axis may regulate the cell proliferation, angiogenesis, cell migration, epithelial to mesenchymal transition, MAPK signaling pathway, and chemokine signaling pathway. Conclusions. CircSLC8A1 functioned as an inhibitor of neoplasm via modulating the miR-21 and might serve as a prospective target for the treatment of PCa.

CircRNA-Associated CeRNAs Regulatory Axes in Retinoblastoma: A Systematic Scoping Review

“…CircCDYL induces apoptosis and suppresses the proliferation of breast cancer cells with a malignant phenotype ( 101 ). The function of circRNAs as TSGs has also been proven in many cancers, including circDDX17 ( 102 ), circ_PLXNB1 ( 103 ), circTADA2A ( 104 ), and Circ-SMARCA5 ( 105 ) in colorectal cancer, circ_0004872 ( 106 ), circDIDO1 ( 107 ), circMAPK1 ( 108 ), circ_0035445 ( 109 ) and circCCDC9 ( 110 ) in gastric cancer, circPLEKHM3 ( 111 ) in ovarian cancer, circ-MYBL2 ( 112 ) in multiple melanoma, and circSLC8A1 ( 113 ) in prostate cancer.…”

Integrated Analysis of the CircRNA-Based ceRNA Network in Renal Fibrosis Induced by Ischemia Reperfusion Injury

“…CircSLC8A1 was reported to be significantly downregulated in cancers, exerting a tumor suppression function via the ceRNA mechanism. In prostate cancer, circSLC8A1 directly interacted with miR-21 to inhibit the proliferation, angiogenesis, cell migration, and EMT behaviors of tumor cells ( Wang et al, 2021 ). In another study, circSlc8a1 was also significantly reduced in bladder cancer tissues and cell lines.…”

Circ_0076305 facilitates prostate cancer development via sponging miR‐411‐5p and regulating PGK1

“…CircRNAs are abnormally expressed in many malignant tumours and have critical roles in regulation of various pathological and physiological processes in cancers (Arnaiz et al, 2019; Vo et al, 2019). Additionally, some circRNAs have been found to be implicated in PCa tumorigenesis via acting as tumour promoters or suppressors (Wang et al, 2021; Xu et al, 2021). Circ_0076305 is derived from progastricsin (PGC) gene and located on chr6:41704448–41712252.…”