Circular Paper Chromatography

Abstract

Circular paper chromatography has many advantages for use in the small laboratory, due to the simplicity of apparatus and techniques employed. The present paper deals with a systematic study of the physical factors which influence R/ for a simple amino acid system. Aside from pH and quality of paper used, which were kept constant, factors that influence Rf significantly are temperature and length of exposed wick. Rate of solvent flow is best controlled by varying wick width.Application of this simple technique to qualitative and quantitative analysis of complex amino acid mixtures-e.g., protein hydrolyzates-is now under investigation. RUTTER(27, 28) has described a modified chromatographic .

Analysis of the specific radioactivity of valine isolated from aminoacyl-transfer ribonucleic acid of rat liver

“…To detect possible errors introduced in this way, we compared the specific radioactivities of valine obtained by our (Cantoni et al, 1962), the ester bond is relatively stable (Davey & Manchester, 1969) and valine itself is sufficiently soluble in ethanol (Saifer, 1971). Adequate controls would be necessary to establish that the isolation described here would yield accurate results with any other amino acid.…”

Chromatography: And Analogous Differential Migration Methods

“…With radial migration as introduced by Brown in 1939 (164), the flow is through the sector of a circle (Figure 11). This technique, which is frequently called sector, radial, ring, or circular paper chromatography (15,54,139,169), has been modified in many wavs, as by the addition of wash liquid through wicks, tabs, capillaries, and dropping funnels (15,54,169). As with paper strips, it serves for the comparison of substances which are added as small spots about the apex of the sector (15,54).…”

Circular-paper chromatography in qualitative analysis

“…Rf values are dependent upon temperature, pH, solvent system, time of development, humidity, etc. ; and these specified conditions must be fulfilled for direct comparison with published Rf values (12,13,14).…”