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A rapid, semiautomatic system of microchemical analysis for the clinical chemistry laboratory has been proposed. Five basic elements of this system are: (1) The use of siliconated-heparinized plasma. (2) The use of the calibrated-pipet-tip buret technic for measuring small (0.10-ml.) samples. (3) The use of the decantation principle as a precision step in making quantitative transfers. (4) The use of automatic syringe pipets for adding constant volumes of reagents, (5) The use of specific enzymatic methods, whenever these are applicable, for the determination of biologic constituents. The analytic system has already been applied to the determination of such important biologic constituents as glucose, urea nitrogen, phosphorus, acid and alkaline phosphatases, sodium and potassium, calcium, and total protein. The semiautomatic system permits the use of microprocedures in a clinical chemistry laboratory by persons of limited technical skill.

Circular paper chromatography has many advantages for use in the small laboratory, due to the simplicity of apparatus and techniques employed. The present paper deals with a systematic study of the physical factors which influence R/ for a simple amino acid system. Aside from pH and quality of paper used, which were kept constant, factors that influence Rf significantly are temperature and length of exposed wick. Rate of solvent flow is best controlled by varying wick width.Application of this simple technique to qualitative and quantitative analysis of complex amino acid mixtures-e.g., protein hydrolyzates-is now under investigation. RUTTER(27, 28) has described a modified chromatographic .

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