Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p

Shen, Xiaoxu; Wei, Yuanhang; You, Guishuang; Liu, Wei; Amevor, Felix Kwame; Zhang, Yao; He, Haorong; Ma, Menggen; Zhang, Yun; Li, Diyan; Zhu, Qing; Yin, Huadong

doi:10.3390/ani11082396

Cited by 18 publications

(10 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the expression of MyHC , MyoG and MEF2C promoted the differentiation of goat SMSCs, while TGFβ1 inhibited the differentiation of the cells [ 27 , 47 , 48 ]. Meanwhile, MyHC is a myogenesis marker protein, in that the area of MyHC-labeled myotubes is proportional to the degree of differentiation of SMSCs and the formation of myotubes [ 13 , 27 ]. These results suggest that circ_003628 promotes the differentiation of goat SMSCs, and further conclude that the circRNA may also increase caprine skeletal muscle mass by promoting differentiation of goat SMSCs.…”

Section: Discussionmentioning

confidence: 99%

“…With the development of RNA-Seq and bioinformatics technologies, thousands of unique circRNAs have been annotated in skeletal muscle tissues in chickens [ 13 ], cattle [ 14 ], goats [ 15 ], pigs [ 16 ], and sheep [ 17 ]. For example, Lei et al [ 18 ] identified 5755 differentially expressed circRNAs in breast muscle tissue of Shouguang chickens between embryonic periods and postnatal periods.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characteristics and Expression of circ_003628 and Its Promoted Effect on Proliferation and Differentiation of Skeletal Muscle Satellite Cells in Goats

Zhen

Wang

Luo

et al. 2022

Animals

View full text Add to dashboard Cite

In our previous a study, circ_003628 was one of the most highly expressed circular RNAs (circRNAs) in the Longissimus dorsi muscle of goats found by RNA-seq, suggesting that the circRNA may be important for caprine muscle growth and development. However, there have been no reports describing the molecular mechanisms by which circ_003628 regulates the activities of goat skeletal muscle satellite cells (SMSCs). In this study, reverse transcriptase-PCR (RT-PCR) and DNA sequencing were used to validate the authenticity of circ_003628, and its characteristics, expression profile and effect on goat SMSCs were also studied using real-time quantitative-PCR (RT-qPCR), EdU, CCK-8 and immunofluorescence assays. Circ_003628 is partially originated from 13 exons, 12 introns and 3’-untranslated regions (UTR) of caprine Myosin Heavy Chain 1 (MYH1), and 25 exons and 5’ UTR of Myosin Heavy Chain 4 (MYH4), as well as intergenic sequences between the two genes. A total of 77.07% of circ_003628 were located in the nuclei of goat SMSCs, while 22.93% were expressed in the cytoplasm. The circRNAs were only expressed in triceps brachii, quadriceps femoris and longissimus dorsi muscle tissues in nine caprine tissues investigated, with the highest expression level in longissimus dorsi muscle. The expression level of circ_003628 gradually increased during differentiation periods of goat SMSCs and reached the maximum on day 6 after differentiation. The small interfering RNA of circ_003628 (named si-circ_003628) inhibited the viability and proliferation of goat SMSCs, and also decreased the expression of four cell proliferation marker genes: paired box 7 (Pax7), cyclin-dependent kinase 2 (CDK2), CDK4 and CyclinD1 in goat SMSCs. Transfection of si-circ_003628 significantly decreased the area of MyHC-labeled myotubes of goat SMSCs, as well as the expression levels of three differentiation marker genes: myosin heavy chain (MyHC), myogenin (MyoG), and myocyte enhancer factor 2C (MEF2C). These results suggest that circ_003628 promotes the viability, proliferation, and differentiation of goat SMSCs, and they also provide an improved understanding of the roles of circ_003628 in skeletal muscle growth and development in goats.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characteristics and Expression of circ_003628 and Its Promoted Effect on Proliferation and Differentiation of Skeletal Muscle Satellite Cells in Goats

Zhen

Wang

Luo

et al. 2022

Animals

View full text Add to dashboard Cite

show abstract

“…It is worth emphasizing that the connection between Focal adhesion and ECM regulates several intracellular pathways of cell motility, proliferation, and differentiation (57). The PI3K-Akt signaling pathway has also been shown to increase skeletal muscle cell proliferation and differentiation (58,59). We hypothesize that these differentially expressed genes enriched in these three signal pathways are engaged in skeletal muscle differentiation functions, resulting in differential expression.…”

Section: Discussionmentioning

confidence: 99%

RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

Zhao

Xue

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Muscle differentiation is an essential link in animal growth and development, and microRNA and mRNA are indispensable in skeletal muscle differentiation. To improve the meat quality and production of the Leizhou goat, it is vital to understand the molecular mechanism by which its skeletal muscle differentiates. By RNA sequencing (RNA-SEQ), we established miRNA-mRNA profiles of Leizhou goats at three stages: fetal day 70, 90, and 120. There were 991 differently expressed mRNAs and 39 differentially expressed miRNAs found, with the differentially expressed mRNAs mainly enriched in calcium ion binding, ECM-receptor interaction, and Focal adhesion. CKM and MYH3, two muscle differentiation markers, were significantly differentially expressed during this period. In addition, we found that chi-miR-129-5p, chi-miR-433, and chi-miR-24-3p co-regulate muscle differentiation with their target genes. Finally, we can confirm that muscle differentiation occurred in Leizhou goat between 90 and 120 days of the fetus. This study is helpful to better explore the molecular mechanism of goat muscle differentiation.

show abstract

“…Thus, we predicted the potential target miRNAs using RNAhybrid software, 23 miRNAs were found may be regulated by circITSN2 (Supplementary Table 4). Previous reports showed that circRNAs and miRNAs in ceRNA regulatory network have opposite expression patterns (Ouyang et al, 2018a;Shen et al, 2021b), and circITSN2 gradually increased during embryonic muscle development. Besides, previously we sequenced the miRNAs of the breast muscle of embryonic chicks at E10, E13, E16, and E19 (SRA database with accession number PRJNA516545).…”

Section: Circitsn2 Acts As a Mirna Sponge Of Mir-218-5pmentioning

confidence: 94%

A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation

Shen

Wei

Liu

et al. 2021

Front. Cell Dev. Biol.

Self Cite

View full text Add to dashboard Cite

Circular RNA (circRNA) is a class of endogenous non-coding RNAs without 5′ and 3′ ends; an increasing number of studies show that circRNA is involved in skeletal muscle development. From our previous sequencing data, the circRNAome in breast muscle of two chicken lines with a distinct rate of muscle development, which included a fast muscle growing broiler (FMGB) and a slow muscle growing layer (SMGL), we found a novel differentially expressed circRNA generated by intersectin 2 (ITSN2) gene (named circITSN2). We verified that circITSN2 is a skeletal muscle-enriched circRNA that promotes chicken primary myoblast (CPM) proliferation and differentiation. Further molecular mechanism analysis of circITSN2 in chicken myogenesis was performed, and we found circITSN2 directly targeting miR-218-5p. Besides, miR-218-5p inhibits CPM proliferation and differentiation, which is contrary to circITSN2. Commonly, circRNAs act as a miRNA sponge to alleviate the inhibition of miRNAs on mRNAs. Thus, we also identified that a downstream gene LIM domain 7 (LMO7) was inhibited by miR-218-5p, while circITSN2 could block the inhibitory effect of miR-218-5p by targeting it. Functional analysis revealed that LMO7 also accelerates CPM proliferation and differentiation, which was similar to circITSN2 but contrary to miR-218-5p. Taken together, these results suggested that circITSN2 promotes chicken embryonic skeletal muscle development via relieving the inhibition of miR-218-5p on LMO7. Our findings revealed a novel circITSN2/miR-218-5p/LMO7 axis in chicken embryonic skeletal muscle development, which expands our understanding of the complex muscle development regulatory network.

show abstract

Circular PPP1R13B RNA Promotes Chicken Skeletal Muscle Satellite Cell Proliferation and Differentiation via Targeting miR-9-5p

Cited by 18 publications

References 34 publications

Characteristics and Expression of circ_003628 and Its Promoted Effect on Proliferation and Differentiation of Skeletal Muscle Satellite Cells in Goats

Characteristics and Expression of circ_003628 and Its Promoted Effect on Proliferation and Differentiation of Skeletal Muscle Satellite Cells in Goats

RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

A Novel Circular RNA circITSN2 Targets the miR-218-5p/LMO7 Axis to Promote Chicken Embryonic Myoblast Proliferation and Differentiation

Contact Info

Product

Resources

About