Circular RNA circ_0003028 contributes to tumorigenesis by regulating GOT2 via miR-1298-5p in non-small cell lung cancer

Guan, Hongjun; Sun, Changpeng; Gu, Yinfeng; Li, Jinjin; Ji, Jie; Zhu, Yongxian

doi:10.1080/21655979.2021.1935064

Cited by 34 publications

(24 citation statements)

References 40 publications

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“…After incubation with PuriMag G-streptavidin magnetic beads at room temperature for 1 h. The magnetic beads were washed and eluted by SDS loading buffer at 100°C for 5 min, followed by western blot analysis. RIP assay was conducted based on previous protocol [ 13 ] by using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit according to manufacturer’s instructions (MERCK, USA).…”

Section: Methodsmentioning

confidence: 99%

Upregulated circRNA_102231 promotes gastric cancer progression and its clinical significance

et al. 2021

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Circular RNAs (circRNAs) are a type of endogenous non-coding RNAs implicated in cancer progression. This study explored the expression levels, clinical implication and possible molecular mechanism of circRNA_102231 in gastric cancer (GC). Gene Expression Omnibus (GEO) was used to analyze differentially expressed circRNAs. CircRNA_102231 expression was verified by qRT-PCR in GC tissues and plasma. The effects of circRNA_102231 was tested by CCK-8, colony formation, EdU and Transwell assays and xenograft tumor model. RNA pull-down and immunoprecipitation (RIP) assays were used to analyze the interaction between circRNA_102231 and IRTKS. CircRNA_102231 expression was significantly upregulated in GC tissue and plasma samples, which can be used as a biomarker for GC diagnosis and prognosis. The function assays showed that circRNA_102231 knockdown inhibited GC cell proliferation and invasion both in vitro and in vivo. CircRNA_102231 was able to bind to IRTKS, increasing IRTKS protein stability, leading to GC progression. Overexpression of IRTKS effectively rescued the reduced cell viability and invasion caused by silencing of circRNA_102231. In sum, our data demonstrate that circRNA_102231 is a novel oncogene in GC and acts as a potential biomarker and therapeutic target for GC patients.

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Section: Methodsmentioning

confidence: 99%

Upregulated circRNA_102231 promotes gastric cancer progression and its clinical significance

et al. 2021

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“…Cell apoptosis assay was performed as described previously [ 12 ]. Briefly, SH-SY5Y cells were exposed to with or without 10 mmol/L mepivacaine.…”

Section: Methodsmentioning

confidence: 99%

MicroRNA-183-5p protects human derived cell line SH-SY5Y cells from mepivacaine-induced injury

Zhou

Zhang

2021

Bioengineered

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With the gradual recognition of the side effects of local anesthetics, the nerve injury caused by local anesthetics has received growing attention. This research intended to delve into miR-183-5p changes in mepivacaine-mediated SH-SY5Y cell injury, as well as its modulatory mechanism on cell apoptosis. RT-qPCR was adopted for assaying miR-183-5p and PDCD4 mRNA expression. Our team respectively transfected miR-183-5p mimic and inhibitor to enhance or inhibit miR-183-5p function. We employed Western blot for detecting PDCD4 protein levels, as well as flow cytometry and Hoechst 33342/PI double staining for determining cell apoptosis rate. Additionally, our crew applied an ELISA kit for measuring TNF-α, IL-1β, IL-6, and IL-8 contents. The level of reactive oxygen species (ROS) production was examined by the Image-iT LIVE Green ROS detection Kit. As well as dual-luciferase reporter experiment for verifying the targeting link of miR-183-5p with PDCD4. In mepivacaine-induced cell apoptosis in SH-SY5Y cells, miR-183-5p expression was downregulated. TNF-α, IL-1β, IL-6, and IL-8 contents were elevated. The rate of apoptosis increased visibly, cleaved caspase-3 and Bax levels waxed, whereas Bcl-2 level waned. MiR-183-5p could alleviate the damaging impact of mepivacaine. Dual-luciferase reporter experiments demonstrated that miR-183-5p directly targeted PDCD4. Collectively, we concluded that a high concentration of mepivacaine can cause SH-SY5Y cell damage, miR-183-5p functions crucially in mepivacaine-mediated cell damage. This study provides a theoretical basis for elucidating the mechanism of mepivacaine-induced nerve cell damage, and overexpressed miR-183-5p likely become a novel strategy to combat mepivacaine-induced nerve damage.

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“…CCK-8 assay was performed as described previously [ 17 ]. The cells were resuspended at 24 h post-transfection, seeded into 96-well plates (2 × 10 4 ), and cultured for 96 h using CCK-8 (Beyotime, Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

The circular RNA circ0005654 interacts with specificity protein 1 via microRNA-363 sequestration to promote gastric cancer progression

Yang

Han

2021

Bioengineered

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Circular RNAs (circRNAs), a group of unique long noncoding RNAs, are involved in gastric carcinogenesis through multiple mechanisms, including interacting with microRNAs (miRNAs). Here, circ0005654, significantly upregulated in gastric cancer (GC), was chosen for further examination. circ0005654 was analyzed by RT-qPCR. The function of circ0005654 in GC cells was substantiated by loss-of-function assays. The mechanism of circ0005654 on miR-363/specificity protein 1 (sp1) axis was evaluated in GC cells by bioinformatics analysis, luciferase reporter, FISH, and ChIP assays. We observed that circ0005654 was enhanced in GC tissues and cells. Overexpression of circ0005654 was correlated with a poor long-term prognosis in patients with GC. Functionally, silencing of circ0005654 remarkably suppressed GC cell proliferation, migration and invasiveness in vitro and tumorigenesis and metastases in vivo. It was also established that circ0005654 served as a miR-363 sponge and enhanced sp1 expression. Furthermore, sp1 promoted GC carcinogenesis by regulating myc transcription to potentiate the Wnt/β-catenin pathway. In conclusion, circ0005654 expedites the GC development via miR-363/sp1/myc/Wnt/βcatenin axis and is a new biomarker for GC treatment regimen.

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Circular RNA circ_0003028 contributes to tumorigenesis by regulating GOT2 via miR-1298-5p in non-small cell lung cancer

Cited by 34 publications

References 40 publications

Upregulated circRNA_102231 promotes gastric cancer progression and its clinical significance

Upregulated circRNA_102231 promotes gastric cancer progression and its clinical significance

MicroRNA-183-5p protects human derived cell line SH-SY5Y cells from mepivacaine-induced injury

The circular RNA circ0005654 interacts with specificity protein 1 via microRNA-363 sequestration to promote gastric cancer progression

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