Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes

Chen, Yajun; Yang, Feng; Fang, Erhu; Xiao, Wenjing; Li, Huanhuan; Li, Dan; Song, Huajie; Wang, Jianqun; Hong, Ming; Wang, Xiaojing; Huang, Kai; Zheng, Liduan; Tong, Qiangsong

doi:10.1038/s41418-018-0220-6

Cited by 254 publications

(218 citation statements)

References 58 publications

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“…Except as RNA sponge, mounting evidence suggested circRNAs could sequester proteins. 16 Here, we constructed a circFOXO3 interaction protein network. By using RBPDB database, eight RNA binding proteins (MBNL1, NONO, SFRS9, RBM4, SFRS1, FUS, EIF4B and RBMX) with relative score ≥ 0.99 were identified to interact with circFOXO3 ( Figure S1A).…”

Section: Identification Of Circfoxo3 Mediated Cerna and Confirmatiomentioning

confidence: 99%

Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p

Kong

Wan

et al. 2019

J Cellular Molecular Medi

116

104

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Circular RNA FOXO3 (CircFOXO3, also termed as Hsa_circ_0006404) is derived from exon 2 of forkhead box O3 (FOXO3) gene, and abnormal expression is shown in different diseases. However, whether circFOXO3 plays important roles in tumorigenesis and progression of prostate cancer (PCa) remains unclear. In this study, we found that circFOXO3 was up‐regulated in both PCa tissues and serum samples. Moreover, circFOXO3 was positively correlated with the Gleason score in PCa samples. CircFOXO3 was observed to be up‐regulated in Gleason score > 6 PCa samples compared with Gleason score = 6 PCa samples. Knock‐down circFOXO3 could remarkably inhibit PCa cell cycle, proliferation and promote cell apoptosis in vitro. Furthermore, we demonstrated circFOXO3 could act as miR‐29a‐3p sponge to up‐regulate SLC25A15 expression by bioinformatics analysis, dual‐luciferase reporter assays and biotinylated RNA pull‐down assays. SLC25A15 could reverse the tumour suppressing roles of knock‐down circFOXO3 in PCa. Of note, we found that miR‐29a‐3p was down‐regulated; however, SLC25A15 was overexpressed in PCa samples compared with normal tissues. In conclusion, circFOXO3 acts as a miR‐29a‐3p sponge to exhibit oncogenic activity that affects the cell cycle and cell apoptosis in PCa through transcriptional up‐regulation of SLC25A15. Our analysis suggests circFOXO3 could act as promising prostate cancer biomarkers.

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Section: Identification Of Circfoxo3 Mediated Cerna and Confirmatiomentioning

confidence: 99%

Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p

Kong

Wan

et al. 2019

J Cellular Molecular Medi

116

104

View full text Add to dashboard Cite

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“…Long noncoding RNA (lncRNAs) and circular RNAs (cir-cRNAs) are important factors in human cancer pathogenesis [15,20,21]. Both lncRNAs and circRNAs lack the ability to encode proteins and circRNAs are characterized by the covalent conjunction and lacking of the 3′ and 5′ end.…”

Section: Discussionmentioning

confidence: 99%

“…To elucidate circRNA-miRNA interaction potentials, bioinformatics databases TargetScan (https ://www.targe tscan .org/) and circinteractome (https ://circi ntera ctome .nia.nih.gov/) were used to predict potential binding sites of miRNAs in circENTPD7 [15,16]. The results of this study found that miR-101-3p may be the target of circENTPD7.…”

Section: Circentpd7 Targets Mir-101-3p As a Mirna Spongementioning

confidence: 97%

A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1

et al. 2020

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Background: Circular RNAs (circRNAs) are identified to play an important role in many human cancers, such as glioblastoma. However, the potential mechanisms underlying the relationship between circRNAs and glioblastoma pathogenesis are still elusive. This study is designed to investigate the role of circRNAs in glioblastoma progression. Methods:The present study is designed to investigate the mechanism by which circRNAs involves in glioblastoma pathogenesis. By using circRNAs microarray, we detected the dysregulated circRNAs and identified an up-regulated circRNA, circENTPD7 in glioblastoma tissues. Cell proliferation was measured using a CCK-8 assay. Cell clone formation ability was assessed with a clone formation test. We used the bioinformatics website to predict circRNA-miRNA and miRNA-mRNA interactions. CircRNA-miRNA interaction was confirmed by dual-luciferase reporter assays and RNA-RNA pulldown assay.Results: circENTPD7 (hsa_circ_0019421) was upregulated in glioblastoma tissues. Kaplan-Meier survival analysis indicated that glioblastoma patients had a poor overall survival when circENTPD7 expression levels were high. Knockdown of circENTPD7 inhibited the motility and proliferation of glioblastoma cells. Moreover, we demonstrated that circENTPD7 acted as a sponge of miR-101-3p to regulate the expression of ROS1 further promoted the proliferation and motility of glioblastoma cells. Conclusions:Taken together, these findings indicate that circRNA circENTPD7 promotes glioblastoma cell proliferation and motility by regulating miR-101-3p/ROS1.

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“…Circular RNAa (circRNAs), a new subtype of ncRNAs, have covalently closed loop structures with a back splice site between 5′-and 3′-end and exhibit higher conservativity than the corresponding linear RNAs duo to resistance to RNase R [7]. Mounting data indicated that circRNAs act critical roles in multiple molecular mechanisms including tumor biomarkers, regulating gene expression, and sponging miRNAs in cancer [8][9][10]. Circ-DONSON [8], circAGO2 [9], circAKT3 [11], circNRIP1 [12], and circDLST [13] are upregulated in GC tissues samples, and their increased expression is associated with TNM stage and poor prognosis in patients with GC [8,11,13].…”

Section: Introductionmentioning

confidence: 99%

“…Mounting data indicated that circRNAs act critical roles in multiple molecular mechanisms including tumor biomarkers, regulating gene expression, and sponging miRNAs in cancer [8][9][10]. Circ-DONSON [8], circAGO2 [9], circAKT3 [11], circNRIP1 [12], and circDLST [13] are upregulated in GC tissues samples, and their increased expression is associated with TNM stage and poor prognosis in patients with GC [8,11,13]. CircAKT3 and circDLST act as the sponges of miR-198/-502-5p to favor the tumorigenesis and cisplatin resistance in GC cells [11,13].…”

Section: Introductionmentioning

confidence: 99%

CircDUSP16 promotes the tumorigenesis and invasion of gastric cancer by sponging miR-145-5p

et al. 2019

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Background Circular RNAs (circRNAs) as a novel subgroup of non-coding RNAs act a critical role in the pathogenesis of gastric cancer (GC). However, the underlying mechanisms by which hsa_circ_0003855 (circDUSP16) contributes to GC are still undocumented. Materials The differentially expressed circRNAs were identified by GEO database. The association of circDUSP16 or miR-145-5p expression with clinicopathological features and prognosis in GC patients was analyzed by FISH and TCGA-seq data set. Loss-and gain-of-function experiments as well as a xenograft tumor model were performed to assess the role of circDUSP16 in GC cells. circDUSP16-specific binding with miR-145-5p was confirmed by bioinformatic analysis, luciferase reporter, and RNA immunoprecipitation assays. Results The expression levels of circDUSP16 were markedly increased in GC tissue samples and acted as an independent prognostic factor of poor survival in patients with GC. Knockdown of circDUSP16 repressed the cell viability, colony formation, and invasive potential in vitro and in vivo, but ectopic expression of circDUSP16 reversed these effects. Moreover, circDUSP16 possessed a co-localization with miR-145-5p in the cytoplasm, and acted as a sponge of miR-145-5p, which attenuated circDUSP16-induced tumor-promoting effects and IVNS1ABP expression in GC cells. MiR-145-5p had a negative correlation with circDUSP16 expression and its low expression was associated with poor survival in GC patients. Conclusions CircDUSP16 facilitates the tumorigenesis and invasion of GC cells by sponging miR-145-5p, and may provide a novel therapeutic target for GC.Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes

Cited by 254 publications

References 58 publications

Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p

Circular RNA circFOXO3 promotes prostate cancer progression through sponging miR‐29a‐3p

A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1

CircDUSP16 promotes the tumorigenesis and invasion of gastric cancer by sponging miR-145-5p

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