Circular RNA Hsa_Circ_0072995 Promotes Breast Cancer Cell Migration and Invasion Through Sponge for Mir-30c-2-3p

Zhang, He-Da; Jiang, Linhong; Hou, Jianglong; Zhou, Siying; Zhong, Shanliang; Zhu, Liming; Wang, Dandan; Yang, Su-Jin; He, Yunjie; Mao, Changfei; Yang, Yong; Wang, Jinyan; Zhang, Qian; Xu, Hanzi; Yu, Dandan; Zhao, Jinmin; Tang, Jinhai; Ji, Zhenling

doi:10.2217/epi-2018-0002

Cited by 60 publications

(42 citation statements)

References 34 publications

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“…19 Analogously, up-regulation of hsa_cir-cRNA_0072995 is determined in breast cancer cells; in contrast, the silencing of hsa_circRNA_0072995 restrained aggressive malignant behaviours in breast cancer. 20 Moreover, hsa_circRNA_002178 silencing inhibited inflammation in vivo through reducing TNF-α and IL-6 levels and prevented tumour growth. Targeting angiogenesis or inflammation before clinical manifestation is in favour of preventing tumour growth and progression.…”

Section: Discussionmentioning

confidence: 99%

“…Our study conducted hsa_circRNA_002178 loss-of-function experiments and finally identified that hsa_cir-cRNA_002178 functioned as a miR-328-3p sponge and impaired miR-328-3p-targeted repression of COL1A1.The first observation was that high hsa_circRNA_002178 expression in breast cancer was associated with poor prognosis.Knockdown of hsa_circRNA_002178 suppressed aggressive malignant behaviours including cell proliferation and angiogenesis. Analogously, up-regulation of hsa_cir-cRNA_0072995 is determined in breast cancer cells; in contrast, the silencing of hsa_circRNA_0072995 restrained aggressive malignant behaviours in breast cancer 20. Analogously, up-regulation of hsa_cir-cRNA_0072995 is determined in breast cancer cells; in contrast, the silencing of hsa_circRNA_0072995 restrained aggressive malignant behaviours in breast cancer 20.…”

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confidence: 99%

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Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA‐328‐3p‐mediated inhibition of COL1A1

Liu

et al. 2020

J Cellular Molecular Medi

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Circular RNAs (circRNAs) are a group of non-coding RNAs implicated in the pathogenesis of cancer progression, which exert their functions via regulation of microRNAs (miRNAs) and genes. The present study uses gain-and loss-of-function approaches to evaluate the functions of hsa_circRNA_002178 in angiogenesis along with energy metabolism and underlying downstream signals. The expression pattern of hsa_cir-cRNA_002178 in clinical breast cancer tissues and its association with prognosis were characterized at first. Next, the energy metabolism and angiogenesis as well as cell viability were evaluated when the expression of hsa_circRNA_002178 in breast cancer cells was knocked down by siRNA. The interaction between hsa_circRNA_002178 and its downstream miR-328-3p was identified, followed by the analysis of their functions in regulation of breast cancer cellular behaviours. The target gene of miR-328-3p was predicted and verified, followed by identifying its role in the breast cancer progression. Higher expression of hsa_circRNA_002178 shared an association with worse prognosis in breast cancer. The inhibition of hsa_circRNA_002178 resulted in reductions in cell viability, energy metabolism and tube formation ability.Hsa_circRNA_002178 could competitively bind to miR-328-3p and down-regulated its expression. Restoration of miR-328-3p eliminated the tumour-promoting effects of hsa_circRNA_002178. COL1A1, as a target of miR-328-3p, could be up-regulated by overexpression of hsa_circRNA_002178. In vivo experiments further confirmed the inhibition of tumour growth and inflammation by silencing hsa_circRNA_002178 or up-regulating miR-328-3p. Taken together, hsa_circRNA_002178 is highlighted as a promising target for breast cancer due to the anti-tumour effects achieved by silencing hsa_circRNA_002178. K E Y W O R D S angiogenesis, breast cancer, circular RNA, COL1A1, energy metabolism, Hsa_ circRNA_002178, MicroRNA-328-3p Additional supporting information may be found online in the Supporting Information section. How to cite this article: Liu T, Ye P, Ye Y, Lu S, Han B. Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA‐328‐3p‐mediated inhibition of COL1A1

Liu

et al. 2020

J Cellular Molecular Medi

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“…CircRNAs have been recognized as imperative regulators in multiple physiological/pathological processes. Although the exact roles of most circRNAs are still unclear, accumulating evidence has documented that some of them can function as oncogenic or tumor suppressive roles in human malignancies, including BC [7][8][9]. For example, Song et al revealed that silencing of circ_0007534 attenuates BC cell progression via affecting miR-593/MUC19 axis [10].…”

Section: Introductionmentioning

confidence: 99%

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

Yao

Leng

et al. 2018

Cell Physiol Biochem

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Background/Aims: Circular RNAs (circRNAs) are a class of non-coding RNAs. They have been proved to be critically involved in tumorigenesis and progression of malignancies through competing endogenous RNA (ceRNA) mechanism. Nevertheless, the exploration between circRNAs and pathogenesis of breast cancer (BC) is limited. Previously, circ_0005230 was identified upregulated in BC tissues screened by circRNA microarray. In the present study, we aimed to investigate the expression pattern, functional role, and mechanism of circ_0005230 in BC. Methods: qRT-PCR was conducted to elucidate the expression levels of circ_0005230 in BC tissues and cells. Additionally, the clinical severity and prognostic value were investigated. CCK-8, colony-forming, flow cytometric assays were performed. Animal study was conducted to validate the in vitro data. What’s more, Transwell assays were induced to detect the cell metastatic properties of circ_0005230 exerts in BC cells. Luciferase reporter assay was used to measure the mechanism of circ_0005230. Results: circ_0005230 was overexpressed in BC tissue specimens and cell lines. The overexpression of circ_0005230 was related to adverse phenotypes in the patients with BC. In addition, circ_0005230 could be regarded as a prognostic predictor in BC patients. In vitro and in vivo data demonstrated the cell growth promoting role of circ_0005230. Moreover, circ_0005230 could also promote cell migratory and invasive capacities. For the mechanism investigation, circ_0005230 was proved to be a sponge of miR-618, and expression of miR-618 could regulate CBX8 expression via targeting the 3’UTR of CBX8. Rescue assays also illustrated an oncogenic function of circ_0005230 in BC via acting as a miR-618 sponge to promote CBX8 expression. Conclusion: circ_0005230/miR-618/CBX8 axis might play a key role in BC tumorigenesis and development.

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“…27 circFBLIM1 plays a vital role in HCC progression. 28 circNT5E functions as an important tumor promoter in glioblastoma tumorigenesis by stimulating tumor proliferation, invasion, migration, and apoptosis, 29 while the circITGA7 can inhibit colorectal cancer growth and metastasis. 30 In terms of potential molecular mechanisms, studies have indicated that circRNAs containing miRNA target sites can serve as miRNA sponges and participate in posttranscriptional regulation of expression.…”

Section: Discussionmentioning

confidence: 99%

“…30 In terms of potential molecular mechanisms, studies have indicated that circRNAs containing miRNA target sites can serve as miRNA sponges and participate in posttranscriptional regulation of expression. For example, hsa_circ_0072995 acts as the sponge of miR-30c-2-3p and promotes breast cancer cell migration and invasion, 29 while circMTO1 suppresses HCC progression by binding to miR-9. 31 hsa_circ_0000337 is located on chr11: 70200406-70202360 with a length of 419 bp and its gene symbol is PPFIA1 (PTPRF interacting protein alpha 1).…”

Section: Discussionmentioning

confidence: 99%

<p>Upregulated circ RNA hsa_circ_0000337 promotes cell proliferation, migration, and invasion of esophageal squamous cell carcinoma</p>

Song¹,

Xu²,

Shi³

et al. 2019

CMAR

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Background As a new class of endogenous ncRNAs, circRNAs have been recently verified to be involved in the carcinogenesis and progression of human cancers. In the current study, we attempted to explore the potential function of a candidate circRNA (hsa_circ_0000337) in esophageal squamous cell carcinoma (ESCC). Patients and methods The altered expression of hsa_circ_0000337 was validated in clinical samples from 48 patients with ESCC. The human esophageal carcinoma cell lines KYSE-150 and TE-1, and the normal human esophageal epithelial cell line (HET-1A) were applied for functional analysis of hsa_circ_0000337. Cell proliferation was measured using the Cell Counting Kit-8 assay and the colony formation assay. Cell invasion and migration were detected by Transwell and wound healing assays, respectively. We further performed bioinformatic analysis and luciferase reporter assays to explore the role of hsa_circ_0000337 as a miRNA sponge. Results hsa_circ_0000337 was significantly upregulated in ESCC tissues compared to adjacent normal-appearing tissues ( P <0.0001). In our in vitro experiment, the expression of hsa_circ_0000337 was higher in TE-1 compared to the normal human esophageal epithelial cell line HET-1A ( P <0.001), but was not significantly different in KYSE-150 ( P >0.05). Knockdown of hsa_circ_0000337 significantly inhibited cell proliferation, migration, and invasion in TE-1 and KYSE-150 cell lines. Bioinformatics predicted and luciferase reporter assay verified that hsa_circ_0000337 could bind to miR-670-5p, a ncRNA involved in carcinogenesis. It is estimated that 21 genes are regulated by miR-670-5p. Conclusion hsa_circ_0000337 was found to be an upregulated circRNA that is related to ESCC and promotes the progression of disease by regulating cell proliferation, migration, and invasion. These findings suggest that this circRNA could be a promising diagnostic biomarker and potential therapeutic target.

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Circular RNA Hsa_Circ_0072995 Promotes Breast Cancer Cell Migration and Invasion Through Sponge for Mir-30c-2-3p

Abstract: Hsa_circ_0072995 may be a novel biomarker for breast cancer, and may function in metastasis of breast cancer.

Cited by 60 publications

References 34 publications

Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA‐328‐3p‐mediated inhibition of COL1A1

Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA‐328‐3p‐mediated inhibition of COL1A1

Increased Expression of Circular RNA circ_0005230 Indicates Dismal Prognosis in Breast Cancer and Regulates Cell Proliferation and Invasion via miR-618/ CBX8 Signal Pathway

<p>Upregulated circ RNA hsa_circ_0000337 promotes cell proliferation, migration, and invasion of esophageal squamous cell carcinoma</p>

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