Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA‐328‐3p‐mediated inhibition of COL1A1

Abstract

Circular RNAs (circRNAs) are a group of non-coding RNAs implicated in the pathogenesis of cancer progression, which exert their functions via regulation of microRNAs (miRNAs) and genes. The present study uses gain-and loss-of-function approaches to evaluate the functions of hsa_circRNA_002178 in angiogenesis along with energy metabolism and underlying downstream signals. The expression pattern of hsa_cir-cRNA_002178 in clinical breast cancer tissues and its association with prognosis were characterized at first. Next, the energy metabolism and angiogenesis as well as cell viability were evaluated when the expression of hsa_circRNA_002178 in breast cancer cells was knocked down by siRNA. The interaction between hsa_circRNA_002178 and its downstream miR-328-3p was identified, followed by the analysis of their functions in regulation of breast cancer cellular behaviours. The target gene of miR-328-3p was predicted and verified, followed by identifying its role in the breast cancer progression. Higher expression of hsa_circRNA_002178 shared an association with worse prognosis in breast cancer. The inhibition of hsa_circRNA_002178 resulted in reductions in cell viability, energy metabolism and tube formation ability.Hsa_circRNA_002178 could competitively bind to miR-328-3p and down-regulated its expression. Restoration of miR-328-3p eliminated the tumour-promoting effects of hsa_circRNA_002178. COL1A1, as a target of miR-328-3p, could be up-regulated by overexpression of hsa_circRNA_002178. In vivo experiments further confirmed the inhibition of tumour growth and inflammation by silencing hsa_circRNA_002178 or up-regulating miR-328-3p. Taken together, hsa_circRNA_002178 is highlighted as a promising target for breast cancer due to the anti-tumour effects achieved by silencing hsa_circRNA_002178. K E Y W O R D S angiogenesis, breast cancer, circular RNA, COL1A1, energy metabolism, Hsa_ circRNA_002178, MicroRNA-328-3p Additional supporting information may be found online in the Supporting Information section. How to cite this article: Liu T, Ye P, Ye Y, Lu S, Han B. Circular RNA hsa_circRNA_002178 silencing retards breast cancer progression via microRNA-328-3p-mediated inhibition of COL1A1.

<p>Downregulation of circNRIP1 Suppresses the Paclitaxel Resistance of Ovarian Cancer via Regulating the miR-211-5p/HOXC8 Axis</p>

“…8,9 In recent years, the role of circRNA in a variety of diseases has been well documented. 10,11 Some studies have indicated that circRNA can function as an important biomarker for cancer treatment, including OC. 12 Paclitaxel (PTX) is one of the most common chemotherapy drugs used for OC treatment.…”

Circ_0072995 promotes cell carcinogenesis via up-regulating miR-149-5p-mediated SHMT2 in breast cancer

“…CircRNA hsa_circ_0052112 induced breast cancer cell migration and invasion by targeting miR-125a-5p [10]. CircRNA hsa_circRNA_002178 promoted cell viability, tube formation and energy metabolism via up-regulating COL1A1 through miR-328-3p [11]. circ_0072995 is a novel identified circRNA, a recent study found circ_0072995 was elevated in breast cancer cells, and promoted cell metastasis by binding to miR-30c-2-3p in vitro [12].…”

CircIL4R facilitates the tumorigenesis and inhibits ferroptosis in hepatocellular carcinoma by regulating the miR‐541‐3p/GPX4 axis

“…In this chapter, we ensured that GPX4 was a target of miR-541-3p in HCC cells, cancers. For instance, hsa_circRNA_00278 regulated breast cancer cellular processes via miR-328-3p/COL1A1 axis (Liu, Ye, Ye, Lu, & Han, 2020); hsa_circRNA_0000069 facilitated cervical cancer proliferation and metastasis via miR-873-5p/TUSC3 axis (Zhang, Chen, Sun, An, & Xi, 2020); and circNTRK2 served as a tumorigenic role in esophageal squamous cell carcinoma by sponging miR-140-3p to upregulate NRIP1 (Chen et al, 2020). Similarly, hsa_circRNA_100084/ miR-23a-5p/IGF2 (J.…”