Circular RNAs in diabetes mellitus and its complications

Fan, Wenqi; Pang, Haipeng; Xie, Zhiguo; Huang, Gan; Zhang, Zhou

doi:10.3389/fendo.2022.885650

Cited by 36 publications

(20 citation statements)

References 142 publications

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“…It is widely held that diabetes mellitus (DM) is one of the leading non-communicable illnesses and is characterized by alteration of glucose metabolism and vascular and neural damage. According to differences in the pathogenic mechanisms, DM is divided into type I and type II ( Fan W. et al, 2022 ). It has been demonstrated that immunocytes and autoimmune antibodies-triggered destruction of pancreatic β-cells and concomitant insulin insufficiency are deeply associated with the onset of type I DM ( Renard, 2022 ).…”

Section: Potentials Of Kd Against Disease Developmentmentioning

confidence: 99%

Advances in the therapeutic application and pharmacological properties of kinsenoside against inflammation and oxidative stress-induced disorders

et al. 2022

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Extensive research has implicated inflammation and oxidative stress in the development of multiple diseases, such as diabetes, hepatitis, and arthritis. Kinsenoside (KD), a bioactive glycoside component extracted from the medicinal plant Anoectochilus roxburghii, has been shown to exhibit potent anti-inflammatory and anti-oxidative abilities. In this review, we summarize multiple effects of KD, including hepatoprotection, pro-osteogenesis, anti-hyperglycemia, vascular protection, immune regulation, vision protection, and infection inhibition, which are partly responsible for suppressing inflammation signaling and oxidative stress. The protective action of KD against dysfunctional lipid metabolism is also associated with limiting inflammatory signals, due to the crosstalk between inflammation and lipid metabolism. Ferroptosis, a process involved in both inflammation and oxidative damage, is potentially regulated by KD. In addition, we discuss the physicochemical properties and pharmacokinetic profiles of KD. Advances in cultivation and artificial synthesis techniques are promising evidence that the shortage in raw materials required for KD production can be overcome. In addition, novel drug delivery systems can improve the in vivo rapid clearance and poor bioavailability of KD. In this integrated review, we aim to offer novel insights into the molecular mechanisms underlying the therapeutic role of KD and lay solid foundations for the utilization of KD in clinical practice.

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Section: Potentials Of Kd Against Disease Developmentmentioning

confidence: 99%

Advances in the therapeutic application and pharmacological properties of kinsenoside against inflammation and oxidative stress-induced disorders

et al. 2022

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“…A study reported that CD3 monoclonal antibody injection could delay the onset of T1DM in children ( 10 ). Accurate prevention and treatment of T1DM requires the identification of key pathogenic genes ( 11 ), which can serve as biological markers for early diagnosis and typing, as well as therapeutic targets.…”

Section: Introductionmentioning

confidence: 99%

Screening of key pathogenic genes of type 1 diabetes in children

Zhan¹,

Xu²,

Chen³

et al. 2023

Transl Pediatr

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Background: The etiology of type 1 diabetes mellitus (T1DM) in pediatric populations remains poorly understood. The key to precise prevention and treatment of T1DM in identifying crucial pathogenic genes.These key pathogenic genes can serve as biological markers for early diagnosis and classification, as well as therapeutic targets. However, there is currently a lack of relevant research on screening key pathogenic genes based on sequencing data and efficient algorithms. Methods:The transcriptome sequencing results of peripheral blood mononuclear cells (PBMCs) of children with T1DM (GSE156035) were downloaded from the Gene Expression Omnibus (GEO) database.The data set contained 20 T1DM samples and 20 control samples. Differentially expressed genes (DEGs) in children with T1DM were selected based on fold change (FC) >1.5 times and adjusted P value <0.05. The weighted gene co-expression network was constructed. Hub genes were screened as modular membership (MM) >0.8 and gene significance (GS) >0.5. Intersection genes of DEGs and hub genes were defined as key pathogenic genes. The diagnostic efficacy of key pathogenic genes was evaluated using receiver operator characteristic (ROC) curves.Results: A total of 293 DEGs were selected. Compared with the control group, 94 genes were downregulated and 199 genes were up-regulated in the treatment group. Black modules (Cor =0.52, P=2e−12) were positively correlated with diabetic traits, whereas brown modules (Cor =−0.51, P=5e−12) and pink modules (Cor =−0.53, P=5e−13) were negatively correlated with diabetic traits. The black module contained 15 hub genes, the pink gene module contained 9 hub genes, and the brown module contained 52 hub genes.The intersection of hub genes and DEGs contained 2 genes, CCL25 and EGFR. The expression of CCL25 and EGFR was low in control samples and high in the test group (P<0.001). The areas under ROC curves (AUCs) of CCL25 and EGFR were 0.852 and 0.867, respectively (P<0.05).Conclusions: Weighted correlation network analysis (WGCNA) was used to identify the key pathogenic genes of T1DM in children, including CCL25 and EGFR, which have good diagnostic efficacy for T1DM in children.

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“…Many differentially expressed circRNAs were found in gingival tissues of individuals with periodontitis, and the circRNA/miRNA/mRNA signal axis plays an important role in periodontitis 9,10 . In addition, the circRNA regulatory network has been reported to be involved in the occurrence and development of diabetes and its complications, such as diabetes mellitus, gestational diabetes mellitus, diabetic retinopathy, diabetic nephropathy, and diabetic cardiomyopathy 11 . However, the role and mechanism of circRNA in the development of periodontitis exacerbated by diabetes have not been reported.…”

Section: Introductionmentioning

confidence: 99%

Circular RNA hsa_circ_0084054 promotes the progression of periodontitis with diabetes via the miR‐508‐3p/PTEN axis

Liu

et al. 2023

J of Periodontal Research

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Background and Objective Diabetes is an important risk factor for periodontitis, and circular RNA (circRNA) may play an important role in aggravating inflammation and accelerating disease progression by regulating miRNA/mRNA. This study aimed to investigate the role and mechanism of the hsa_circ_0084054/miR‐508‐3p/PTEN axis in the progression of periodontitis with diabetes. Methods First, circRNA sequencing was used to screen the differentially expressed circRNAs of periodontal ligament cells (PDLCs) treated with high glucose and/or Porphyromonas gingivalis lipopolysaccharide (LPS) in vitro, and the overtly differentially expressed hsa_circ_0084054 was selected and was also verified in periodontal ligament (PDL) tissue from periodontitis patients with diabetes. Then, its ring structure was tested by Sanger sequencing, RNase R, and actinomycin D assays. The bioinformatics analysis, dual luciferase reporter assay, and RIP assay were used to explore the interaction of hsa_circ_0084054/miR‐508‐3p/PTEN axis, whose effects on inflammation, oxidative stress, and apoptosis of PDLCs were evaluated through the measurement of inflammatory factors, reactive oxygen species (ROS), total superoxide dismutase (SOD), malondialdehyde (MDA), and Annexin V/PI assay. Results By high‐throughput sequencing, it was found that hsa_circ_0084054 was significantly increased in HG + LPS group compared with control group and LPS group, which was also verified in periodontal ligament (PDL) tissue from periodontitis patients with diabetes. Silencing hsa_circ_0084054 in PDLCs decreased the expression of inflammatory factors (IL‐1β, IL‐6, TNF‐α), the levels of ROS and MDA, and the proportion of apoptotic cells; conversely, SOD activity was enhanced. In addition, we found that hsa_circ_0084054 could up‐regulate the expression of PTEN through sponge miR‐508‐3p to inhibit AKT phosphorylation, finally trigger the aggravation of oxidative stress and inflammation in periodontitis patients with diabetes. Conclusion hsa_circ_0084054 can aggravate inflammation and promote the progression of periodontitis with diabetes by regulating miR‐508‐3p/PTEN signaling axis, which may serve as a new target for the intervention of periodontitis with diabetes.

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Circular RNAs in diabetes mellitus and its complications

Cited by 36 publications

References 142 publications

Advances in the therapeutic application and pharmacological properties of kinsenoside against inflammation and oxidative stress-induced disorders

Advances in the therapeutic application and pharmacological properties of kinsenoside against inflammation and oxidative stress-induced disorders

Screening of key pathogenic genes of type 1 diabetes in children

Circular RNA hsa_circ_0084054 promotes the progression of periodontitis with diabetes via the miR‐508‐3p/PTEN axis

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