Circulating concentrations of α- and β-chemokines in neonatal sepsis

Manoura, Antonia; Gourgiotis, Dimitrios; Galanakis, Emmanouil; Matalliotakis, Emmanouil; Hatzidaki, Eleftheria; Korakaki, Eftichia; Saitakis, Emmanouil; Marmarinos, Antonios; Giannakopoulou, Christine

doi:10.1016/j.ijid.2010.03.015

Cited by 16 publications

(9 citation statements)

References 21 publications

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“…For instance, in a study of multiple myeloma that used an ELISA system, control values (in pg/mL) for epithelial neutrophil-activating protein-78 (ENA-78), GRO-α, HGF, and vascular endothelial growth factor (VEGF) ranged higher than in our control patients [7,8]. The same trend was reported in neonatal studies with that system [9].…”

Section: Discussionsupporting

confidence: 69%

Cytokine and Chemokine Patterns Across 100 Days after Hematopoietic Stem Cell Transplantation in Children

DiCarlo

Agarwal-Hashmi

Shah

et al. 2014

Biology of Blood and Marrow Transplantation

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We mapped the cytokine response to hematopoietic stem cell transplantation (HSCT) by assaying 51 cytokines and chemokines each week for 100 days in 51 children receiving allogeneic (n = 44) or autologous HSCT (n = 7). Assay values were reported as mean fluorescence intensity (MFI). Log transformation converted MFI to clinically relevant measures (ie, pg/mL). We searched for potential markers of transplant complications by using mixed treatment by subject analysis of variance. Global cytokine secretion in HSCT recipients was significantly lower than in concurrent control patients (n = 11). Coincident with the nadir in WBC count, the concentration of many cytokines declined further by the second and third week. All analytes (except monokine induced by gamma interferon [MIG]) subsequently rebounded by week 4 (coincident with engraftment and recovery of WBC count) but often still remained well below control levels. Concurrent with the collective nadir of multiple cytokines, monocyte chemoattractant protein 1 (MCP-1), growth-regulated oncogene alpha (GRO-a), and leptin surged during weeks 2 to 4. High levels of leptin persisted throughout the 100 post-transplant days. Also during weeks 2 to 4, hepatocyte growth factor (HGF) and IL-6 surged in children with complications but not in those without complications. The peak in HGF was more pronounced in veno-occlusive disease (VOD). HGF and IL-6 secretion rose at least 2 weeks before the clinical diagnosis of VOD or graft-versus-host disease (GVHD). From week 4 onward in all groups, the MFI of the cytokine resistin increased to 5 to 15 times above concurrent control. HGF has now emerged in 3 or more biomarker discovery efforts for GVHD (and in our population for VOD as well). HGF (with or without IL-6) should be investigated as a potential predictive biomarker of VOD or GVHD. Alternatively, the hyperinflammatory “signature” provided by a multicytokine assay may be predictive.

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Section: Discussionsupporting

confidence: 69%

Cytokine and Chemokine Patterns Across 100 Days after Hematopoietic Stem Cell Transplantation in Children

DiCarlo

Agarwal-Hashmi

Shah

et al. 2014

Biology of Blood and Marrow Transplantation

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“…Taking into account the fact that in healthy children serum levels of IL-17 increase with age [28], low or undetectable IL-17 levels observed in neonates may mirror the immaturity of the neonatal immune system to launch an effective Th17 response. Most of the previous studies showed lower RANTES concentration in septic neonates compared with healthy subjects [19][20][21][22], though diametrically opposite results have also been reported in the setting of EONS [23]. In the current study the median concentration of this chemokine was lower in EONS group then in non-infected neonates, but the difference did not reach statistical significance.…”

Section: C5a (Ng/ml) Rantes (Ng/ml)contrasting

confidence: 84%

“…An inverse correlation has been reported between the levels of RANTES and disease severity, and mortality in patients with sepsis and lower levels of this chemokine were found in non-surviving patients, suggesting its beneficial pro-inflammatory role in host response to infection [18]. A few studies reported RANTES levels in infected neonates [19][20][21][22] including those with EONS [20,22]. Most of them documented lower RANTES levels in infected neonates compared to non-infected controls.…”

Section: Introductionmentioning

confidence: 99%

Assessment of interleukin-17A, C5a and RANTES for early diagnosis of neonatal sepsis – a preliminary study

Kasztelewicz¹

2016

Preprint

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the aim of the present study was to investigate serum levels of novel markers: interleukin 17a (il-17a), anaphylatoxin C5a and chemokine regulated upon activation normal t-cell expressed and secreted (rantes) in neonates with clinically suspected early-onset neonatal sepsis (eons), and to compare their values with those of non-infected neonates. eighteen neonates with clinical signs and symptoms of eons were enrolled in this study. Fifty healthy, non-infected neonates served as the control group. in all neonates serum levels of il-17a, C5a and rantes were measured by solid-phase sandwich enzyme-linked immunosorbent assay (elisa). at the time of investigation serum levels of anaphylatoxin C5a were significantly higher in neonates with clinical symptoms of eons than in non-infected neonates (median 65.35 vs. 50.4 ng/ml, p = 0.034), whereas levels of rantes were similar and levels of il-17a were under detection limit of the method. Based on these preliminary results, serum levels of C5a may be a useful marker of inflammation in early onset neonatal sepsis. Because traditional methods of microbiological diagnostics in eons are frequently unsuccessful, the search for an alternative laboratory biomarkers is of great clinical importance. thus, there is a strong need for further studies evaluating usefulness of this anaphylatoxin in eons diagnosis on a larger group of patients.

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“…Our results showed that serum CXCL13, CXCL1, CXCL2, CXCL5, CXCL6, CXCL8, CXCL16, CCL27, CCL2, CCL8, CCL3, CCL20, CCL23, CCL27, and CX3CL1 levels were signi cantly increased in neonates with sepsis compared to those in the control group. Manoura et al also found that the levels of serum CXCL1 and CXCL5 were higher in neonates with sepsis [18]. Among these chemokines, CXCL1, CXCL2, CXCL5, CXCL6, and CXCL8 are potent chemoattractants for neutrophils.…”

Section: Discussionmentioning

confidence: 93%

Expression of Serum Cytokines Profile in Neonatal Sepsis

Kuang

Chen

Huang

et al. 2020

Preprint

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BackgroundSepsis remains a major cause of neonatal death, but its underlying pathological mechanisms are poorly understood. Methods To characterize the serum cytokine/chemokine profile in neonates with sepsis, we enrolled 40 full-term neonates with sepsis and 19 neonates without infection as controls. Forty cytokines/chemokines in serum were analyzed using the Luminex Bead Immunoassay System. Serum IL-17 was measured using an enzyme-linked immunosorbent assay. ResultsOur results showed that serum IL-6, IL-8, TNF-α, IL-1β, MIF, CXCL13, CXCL1, CXCL2, CXCL5, CXCL6, CXCL16, CCL27, CCL2, CCL8, CCL3, CCL20, CCL23, CCL27, and CX3CL1 levels were significantly increased in neonates with sepsis compared to those in the control group (all p<0.05). The levels of serum CXCL6, CXCL1, IL-6, CXCL10, CXCL11, CCL20, and IL-17 were higher in late-onset sepsis (LOS) than in early-onset sepsis (EOS) (all p<0.05). Conversely, serum IL-16, CXCL16, and CCL22 were lower in LOS than in EOS (all p<0.05). The levels of CX3CL1, CXCL2, CCL8, and TNF-α were all positively correlated with SOFA scores. ConclusionOur findings revealed that excessive pro-inflammatory cytokines might be involved in neonatal sepsis. In addition, chemokines significantly increased the recruitment of immune cells after infection to participate in the anti-infection defense of neonates, but this could lead to damage.

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Circulating concentrations of α- and β-chemokines in neonatal sepsis

Abstract: Our findings suggest up-regulation of GRO-α and down-regulation of RANTES at the onset of a septic episode, similar to the response pattern observed in septic adults. Both term and preterm neonates appear to have the potential to elicit a chemotactic response to infection.

Cited by 16 publications

References 21 publications

Cytokine and Chemokine Patterns Across 100 Days after Hematopoietic Stem Cell Transplantation in Children

Cytokine and Chemokine Patterns Across 100 Days after Hematopoietic Stem Cell Transplantation in Children

Assessment of interleukin-17A, C5a and RANTES for early diagnosis of neonatal sepsis – a preliminary study

Expression of Serum Cytokines Profile in Neonatal Sepsis

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