Circulating Endothelial Cells as a Marker of Vascular Dysfunction in Patients With Systemic Lupus Erythematosus by Real-Time Polymerase Chain Reaction

Attia, Fadia M.; Maaty, Ahmed; Kalil, Fawzy

doi:10.5858/arpa.2010-0731-oa

Cited by 12 publications

(12 citation statements)

References 23 publications

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“…Other potential sources presented included hairs, fibers, stain precipitate, bacteria, endothelial cells, and sporozoan microgametes. Some basic features regarding these additional sources of blood smear contamination were presented, and salient features are found in the attached references: hairs and fibers, 67,68 stain precipitate, 69 bacteria, 70,71 endothelial cells, 72 -74 and sporozoan microgametes. 75 -78 In summary, the take-home message regarding the spores of helicosporous fungi is that they are air-borne or water-based contaminants in laboratories and they may be mistaken for microfilariae in stained blood smears.…”

Section: Blood Smear Contaminantmentioning

confidence: 99%

Proceedings of the 2019 National Toxicology Program Satellite Symposium

et al. 2019

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The 2019 annual National Toxicology Program Satellite Symposium, entitled “Pathology Potpourri,” was held in Raleigh, North Carolina, at the Society of Toxicologic Pathology’s 38th annual meeting. The goal of this symposium was to present and discuss challenging diagnostic pathology and/or nomenclature issues. This article presents summaries of the speakers’ talks along with select images that were used by the audience for voting and discussion. Various lesions and topics covered during the symposium included aging mouse lesions from various strains, as well as the following lesions from various rat strains: rete testis sperm granuloma/fibrosis, ovarian cystadenocarcinoma, retro-orbital schwannoma, periductal cholangiofibrosis of the liver and pancreas, pars distalis hypertrophy, chronic progressive nephropathy, and renal tubule regeneration. Other cases included polyovular follicles in young beagle dogs and a fungal blood smear contaminant. One series of cases challenged the audience to consider how immunohistochemistry may improve the diagnosis of some tumors. Interesting retinal lesions from a rhesus macaque emphasized the difficulty in determining the etiology of any particular retinal lesion due to the retina’s similar response to vascular injury. Finally, a series of lesions from the International Harmonization of Nomenclature and Diagnostic Criteria Non-Rodent Fish Working Group were presented.

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Section: Blood Smear Contaminantmentioning

confidence: 99%

Proceedings of the 2019 National Toxicology Program Satellite Symposium

et al. 2019

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“…Murine models of lupus showed that NAC treatment suppressed autoantibody formation, reduced nephritis and prolonged survival [72]. Several groups have shown that the decrease in intracellular glutathione has been associated with many clinical features like, nephritis [14], CNS [103], endothelial activation in cardiovascular and cerebrovascular involvement [104] in SLE patients. Administration of NAC has shown beneficial effect in mild SLE patients in terms of decreasing lipid peroxidation, improve CNS complication [103], endothelial function [104,105] in patients with cerebrovascular involvement.…”

Section: Reviewmentioning

confidence: 99%

Oxidative stress and its biomarkers in systemic lupus erythematosus

et al. 2014

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Systemic lupus erythematosus (SLE) is an autoimmune inflammatory disease whose etiology remains largely unknown. The uncontrolled oxidative stress in SLE contributes to functional oxidative modifications of cellular protein, lipid and DNA and consequences of oxidative modification play a crucial role in immunomodulation and trigger autoimmunity. Measurements of oxidative modified protein, lipid and DNA in biological samples from SLE patients may assist in the elucidation of the pathophysiological mechanisms of the oxidative stress-related damage, the prediction of disease prognosis and the selection of adequate treatment in the early stage of disease. Application of these biomarkers in disease may indicate the early effectiveness of the therapy. This review is intended to provide an overview of various reactive oxygen species (ROS) formed during the state of disease and their biomarkers linking with disease. The first part of the review presents biochemistry and pathophysiology of ROS and antioxidant system in disease. The second part of the review discusses the recent development of oxidative stress biomarkers that relates pathogenesis in SLE patients and animal model. Finally, this review also describes the reported clinical trials of antioxidant in the disease that have evaluated the efficacy of antioxidant in the management of disease with ongoing conventional therapy.

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“…Recently, circulating endothelial cells (CECs) have been established as markers of endothelial damage or dysfunction [9]. CEC levels increase in many kinds of disorders such as cardiovascular [10], [11], autoimmune [12], and infectious diseases [13], as well as in cancer [14]. In cancer patients, CEC number correlates with tumor progression [15] and constitutes a promising tool for monitoring disease activity, with potential for the assessment of prognosis and response to treatment.…”

Section: Introductionmentioning

confidence: 99%

Circulating Endothelial Cells and Procoagulant Microparticles in Patients with Glioblastoma: Prognostic Value

et al. 2013

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AimCirculating endothelial cells and microparticles are prognostic factors in cancer. However, their prognostic and predictive value in patients with glioblastoma is unclear. The objective of this study was to investigate the potential prognostic value of circulating endothelial cells and microparticles in patients with newly diagnosed glioblastoma treated with standard radiotherapy and concomitant temozolomide. In addition, we have analyzed the methylation status of the MGMT promoter.MethodsPeripheral blood samples were obtained before and at the end of the concomitant treatment. Blood samples from healthy volunteers were also obtained as controls. Endothelial cells were measured by an immunomagnetic technique and immunofluorescence microscopy. Microparticles were quantified by flow cytometry. Microparticle-mediated procoagulant activity was measured by endogen thrombin generation and by phospholipid-dependent clotting time. Methylation status of MGMT promoter was determined by multiplex ligation-dependent probe amplification.ResultsPretreatment levels of circulating endothelial cells and microparticles were higher in patients than in controls (p<0.001). After treatment, levels of microparticles and thrombin generation decreased, and phospholipid-dependent clotting time increased significantly. A high pretreatment endothelial cell count, corresponding to the 99th percentile in controls, was associated with poor overall survival. MGMT promoter methylation was present in 27% of tumor samples and was associated to a higher overall survival (66 weeks vs 30 weeks, p<0.004).ConclusionLevels of circulating endothelial cells may have prognostic value in patients with glioblastoma.

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Circulating Endothelial Cells as a Marker of Vascular Dysfunction in Patients With Systemic Lupus Erythematosus by Real-Time Polymerase Chain Reaction

Abstract: Endothelial dysfunction is present in SLE patients even in the absence of traditional cardiovascular risk factors due to disease activity.

Cited by 12 publications

References 23 publications

Proceedings of the 2019 National Toxicology Program Satellite Symposium

Proceedings of the 2019 National Toxicology Program Satellite Symposium

Oxidative stress and its biomarkers in systemic lupus erythematosus

Circulating Endothelial Cells and Procoagulant Microparticles in Patients with Glioblastoma: Prognostic Value

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