Circulating inflammatory miRNA signature in response to different doses of aerobic exercise

Abstract

While moderate acute exercise has been associated with strong anti-inflammatory mechanisms, strenuous exercise has been linked to deleterious inflammatory perturbations. It is therefore fundamental to elucidate the mechanisms that regulate the exercise-induced inflammatory cascade. Information on novel regulators such as circulating inflammatory microRNAs (c-inflammamiRs) is incomplete. In this study, we evaluated the response of a panel of c-inflammamiRs to different doses of acute aerobic exercise. We first studied the exercise-induced inflammatory cascade in serum samples of nine active middle-aged males immediately before and after (0 h, 24 h, 72 h) 10-km, half-marathon, and marathon races. Next, we analyzed the circulating profile of 106 specific c-inflammamiRs immediately before) and after (0 h, 24 h) 10-km (low inflammatory response) and marathon (high inflammatory response) races. Analysis of classical inflammatory parameters revealed a dose-dependent effect of aerobic exercise on systemic inflammation, with higher levels detected after marathon. We observed an increase in miR-150-5p immediately after the 10-km race. Levels of 12 c-inflammamiRs were increased immediately after the marathon (let-7d-3p, let-7f-2-3p, miR-125b-5p, miR-132-3p, miR-143-3p, miR-148a-3p, miR-223-3p, miR-223-5p, miR-29a-3p, miR-34a-5p, miR-424-3p, and miR-424-5p). c-inflammamiRs returned to basal levels after 24 h. Correlation and in silico analyses supported a close association between the observed c-inflammamiR pattern and regulation of the inflammatory process. In conclusion, we found that different doses of acute aerobic exercise induced a distinct and specific c-inflammamiR response, which may be associated with control of the exercise-induced inflammatory cascade. Our findings point to c-inflammamiRs as potential biomarkers of exercise-induced inflammation, and hence, exercise dose.

Circulating microRNAs predict future fatal myocardial infarction in healthy individuals – The HUNT study

“…Upregulation of miR-424-5p promotes down-stream processes associated with hypoxia, like angiogenesis and erythropoiesis to overcome perfusion deficits [52]. Circulating miR-424-5p has previously also been considered as a marker for inflammation, and high levels have been detected after a marathon race [53]. Despite small sub-groups, miR-424-5p seems to be more closely associated with early events (AMI that occurs 0-5 years from blood sampling) compared to later events (MI that occurs 6-10 years from blood sampling), but this has to be further investigated in larger populations.…”

The role of diet and exercise in the transgenerational epigenetic landscape of T2DM

“…Acute exercise alters the expression of a number of mi RNAs that are enriched in skeletal muscle, which might modulate the abundance of proteins that regulate glucose and lipid metabolism by fine tuning the expression of target genes to ultimately improve insulin sensitivity. Beyond tissue-specific regulation, circulating mi RNAs released from a variety of tissues including, but not limited to, skeletal muscle, adipose tissue and liver form the basis of a communication system by which gene expression signatures in one organ might be influenced by other exercise-responsive tissues [108][109][110] . The diversity in the different mi RNAs released into the circulation in response to different modes and intensities of exercise or during the recovery period from exercise in trained versus untrained individuals might contribute to the diverse metabolic, cardiovascular and other whole-body adaptations that occur in conjunction with an acute exercise bout or from regular exercise training 62 .…”

Circulating MicroRNAs as Potential Biomarkers of Exercise Response

“…Physical exercise and training induce numerous physiological alterations in cardiovascular system, angiogenesis, cell metabolism, inflammation and muscle remodeling. To date, 17 studies investigating the influence of physical exercise on c-miRNAs levels in plasma and serum have been published [90,91,92,93,94,95,96,97,98,99,100,101,102,103,104,105,106]. …”

“…The influence of marathon running on c-miRNAs expression has been observed in seven studies [91,92,93,94,95,96,101]. Detected myomiRs were upregulated in all of the studies except one focused on inflammatory c-miRNAs [94].…”

“…Detected myomiRs were upregulated in all of the studies except one focused on inflammatory c-miRNAs [94]. …”