Circulating Intercellular Adhesion Molecule-1 in Patients with Systemic Sclerosis

Hasegawa

The Journal of Immunology

et al. 2007

The tight-skin (TSK/+) mouse, a genetic model for systemic sclerosis, develops cutaneous fibrosis. Although a fibrillin 1 gene mutation and immunological abnormalities have been demonstrated, the roles of adhesion molecules have not been investigated. To directly assess roles of adhesion molecules in skin fibrosis, TSK/+ mice lacking L-selectin and/or ICAM-1 were generated. The deficiency of ICAM-1, but not L-selectin, significantly suppressed (∼48%) the development of skin sclerosis in TSK/+ mice. Similarly, ICAM-1 antisense oligonucleotides inhibited skin fibrosis in TSK/+ mice. Although T cell infiltration was modest into the skin of TSK/+ mice, ICAM-1 deficiency down-regulated this migration, which is consistent with the established roles of endothelial ICAM-1 in leukocyte infiltration. In addition, altered phenotype or function of skin fibroblasts was remarkable and dependent on ICAM-1 expression in TSK/+ mice. ICAM-1 expression was augmented on TSK/+ dermal fibroblasts stimulated with IL-4. Although growth or collagen synthesis of TSK/+ fibroblasts cultured with IL-4 was up-regulated, it was suppressed by the loss or blocking of ICAM-1. Collagen expression was dependent on the strain of fibroblasts, but not on the strain of cocultured T cells. Thus, our findings indicate that ICAM-1 expression contributes to the development of skin fibrosis in TSK/+ mice, especially via ICAM-1 expressed on skin fibroblasts.

Section: S Ystemic Sclerosis (Ssc)mentioning

confidence: 99%

Intercellular Adhesion Molecule-1 Deficiency Attenuates the Development of Skin Fibrosis in Tight-Skin Mice

Hasegawa

The Journal of Immunology

et al. 2007

“…Both intra-and inter-assaY coefficients of variation were less than 10% in our hands, indicating a high reproducibility of the assay. In addition, serum levels of sICAM-1 and sIL-2R were measured using ELISA methods (Bender MedSystems, and T-cell Sciences, Cambridge, MA, respectively), as described [33].…”

Section: Determination Of Ses Sicam-1 and Sil-2r Serum Concentrationsmentioning

confidence: 99%

Levels of the circulating cell adhesion molecule E-selectin and disease progression in HIV infection

Tzavara¹,

Sipsas²,

Kosmopoulou³

et al. 1995

Infection

The levels of soluble form of E-Selectin (sEs), or endothelial-leukocyte adhesion molecule-1, were measured in 96 sera derived from 72 HIV-infected patients at different stages of the disease, 60 healthy blood donors, and 50 HIV-negative patients with infections, using a quantitative ELISA. Levels of sEs in HIV-infected individuals without AIDS, according to the 1993 classification system of the Centers for Disease Control, were higher than normal (mean +/- SEM 48 +/- 4 versus 35 +/- 3 ng/ml, p = 0.003). Patients with established AIDS, who were afebrile and had no evidence of acute concurrent infection, had even higher sEs serum levels (70 +/- 9 ng/ml, p = 0.009, compared to those without AIDS). A significant increase in clinical category disease progression was present. Individual concentrations of sEs correlated directly with levels of soluble intercellular adhesion molecule-1 (p < 0.00001) and IL-2 receptor (p = 0.001), but not with CD4+ T-cell counts. Zidovudine treatment was not associated with changes in sEs serum levels. Elevated sEs levels were also found in HIV-seronegative patients with other bacterial and protozoal infections. Since sEs is a biologically active molecule, further studies should investigate the pathogenetic significance of circulating sEs in HIV-related disease progression, and assess the prognostic value of sEs determination for these patients.

“…It is known that thyroid hormones influence the secretion and degradation of several proteins such as fibronectin, angiotensinconverting enzyme and factor VIII-related antigen [34,35]. Shedding of the sICAM-1 into the circulation can therefore be explained as the results of proteolytic cleavage of the membrane bound molecule due to inflammation or the effect of thyroid hormones on vascular endothelium at unrelated sites [21]. The demonstration of shedding of several tissue-specific isoforms of the ICAM-1 molecule supports this view [36].…”

Section: Discussionmentioning

confidence: 99%

“…An increased concentration of circulating sICAM-1 was demonstrated in patients with systemic sclerosis, malignant disease, insulin dependent diabetes mellitus and idiopathic pulmonary fibrosis [21][22][23][24]. Heufelder and Balm [19] and recently De Bellis et al [25] reported elevated circulating sI-CAM--levels in patients with Graves' disease (GD) with or without ophthalmopathy.…”

Section: Intercellularmentioning

confidence: 99%

See 1 more Smart Citation

Soluble Intercellular Adhesion Molecule-1 Concentrations in Patients with Subacute Thyroiditis and in Patients with Graves' Disease with or without Ophthalmopathy.

et al. 1996

Abstract. Increased circulating soluble ICAM-1 (sICAM-1) levels has been previously reported in Graves' disease (GD) patients with or without ophthalmopathy (GO) and in patients with toxic nodular goiter but not in patients with subacute thyroiditis. Conflicting results have also been reported about the usefulness of sICAM-1 levels as a marker for the activity of hyperthyroidism.We have therefore determined sICAM-1 levels by a sandwich enzyme linked immunosorbent assay (ELISA) method in 10