Circulating interleukin-18: A specific biomarker for atherosclerosis-prone patients with metabolic syndrome

Yamaoka‐Tojo, Minako; Tojo, Taiki; Wakaume, Kazuki; Kameda, Ryo; Nemoto, Shinji; Takahira, Naonobu; Masuda, Takashi; Izumi, Tohru

doi:10.1186/1743-7075-8-3

Cited by 34 publications

(24 citation statements)

References 51 publications

(57 reference statements)

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“…It was postulated that exercise may lower IL-18 concentration via insulin-signaling modification [28]. A significant decrease in the concentration of IL-18 has been observed in cyclists 24 hours after completing a 230 km road race [29]. On the other hand, increased concentrations of IL-18 have been associated with several components of the metabolic syndrome and used as a factor in predicting diabetes, atherosclerosis, and cardiovascular disorders [30–32].…”

Section: Discussionmentioning

confidence: 99%

Differences in Plasma Cytokine Levels between Elite Kayakers and Nonathletes

Borges

Rama

Pedreiro

et al. 2013

BioMed Research International

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Regular moderate exercise has been shown to have anti-inflammatory effects that help prevent several chronic diseases. However, the effects of chronic training an elite athletes have not been the focus of much research. This study aimed to determine whether there were differences in cytokine levels (IL-1β, IL-1ra, IL-6, IL-10, IL-18, IFN-γ, and TNF-α) in circulating peripheral blood (PB) between elite kayakers and nonathletes. Subjects were 13 elite male kayakers, aged 20.0 ± 3 years, with average body mass of 75.0 ± 7.9 kg and 177.3 ± 7.1 cm height and with a VO2max of 58.3 ± 7.8 mL·kg−1·min−1. The nonathletes were 7 men, aged 18.2 ± 1.1 years, body mass of 81.3 ± 13.8 kg, and 171.9 ± 4.5 cm height. Blood samples were collected after six weeks of offtraining and before the start of a new training season. PB leukocyte populations were determined by flow cytometry. Cytokine levels were quantified by ELISA. When nonathletes were compared with the kayakers, the latter exhibited lower plasma concentrations of IL-1β, IL-18, and IFN-γ as well as a lower concentration of IL-1ra. Positive correlations between IL-18 and B cells in the athletes were also found. These results seem to reinforce the anti-inflammatory role of regular training.

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Section: Discussionmentioning

confidence: 99%

Differences in Plasma Cytokine Levels between Elite Kayakers and Nonathletes

Borges

Rama

Pedreiro

et al. 2013

BioMed Research International

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“…Dutot et al [27] observed that high glucose induced ROS overproduction in retinal pigment epithelial cells (RPEs). In contrast, Busik et al [28] reported that high glucose does not augment ROS generation.…”

Section: Discussionmentioning

confidence: 99%

Effect of essential fatty acids on glucose-induced cytotoxicity to retinal vascular endothelial cells

Shen

Das

et al. 2012

Lipids Health Dis

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BackgroundDiabetic retinopathy is a major complication of dysregulated hyperglycemia. Retinal vascular endothelial cell dysfunction is an early event in the pathogenesis of diabetic retinopathy. Studies showed that hyperglycemia-induced excess proliferation of retinal vascular endothelial cells can be abrogated by docosahexaenoic acid (DHA, 22:6 ω-3) and eicosapentaenoic acid (EPA, 20:5 ω-3). The influence of dietary omega-3 PUFA on brain zinc metabolism has been previously implied. Zn2+ is essential for the activity of Δ6 desaturase as a co-factor that, in turn, converts essential fatty acids to their respective long chain metabolites. Whether essential fatty acids (EFAs) α-linolenic acid and linoleic acid have similar beneficial effect remains poorly understood.MethodsRF/6A cells were treated with different concentrations of high glucose, α-linolenic acid and linoleic acid and Zn2+. The alterations in mitochondrial succinate dehydrogenase enzyme activity, cell membrane fluidity, reactive oxygen species generation, SOD enzyme and vascular endothelial growth factor (VEGF) secretion were evaluated.ResultsStudies showed that hyperglycemia-induced excess proliferation of retinal vascular endothelial cells can be abrogated by both linoleic acid (LA) and α-linolenic acid (ALA), while the saturated fatty acid, palmitic acid was ineffective. A dose–response study with ALA showed that the activity of the mitochondrial succinate dehydrogenase enzyme was suppressed at all concentrations of glucose tested to a significant degree. High glucose enhanced fluorescence polarization and microviscocity reverted to normal by treatment with Zn2+ and ALA. ALA was more potent that Zn2+. Increased level of high glucose caused slightly increased ROS generation that correlated with corresponding decrease in SOD activity. ALA suppressed ROS generation to a significant degree in a dose dependent fashion and raised SOD activity significantly. ALA suppressed high-glucose-induced VEGF secretion by RF/6A cells.ConclusionsThese results suggest that EFAs such as ALA and LA may have beneficial action in the prevention of high glucose-induced cellular damage.

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“…One study suggested, increase in the serum level of IL-18, as a predictor of progression of diabetic nephropathy and cardiovascular diseases [Nakamura et al, 2005]. Moreover, IL-18 has been also suggested as a useful biomarker of the clinical manifestations of metabolic syndrome and CAD risk factors [Trøseid et al, 2010;Yamaoka-Tojo et al, 2011].…”

mentioning

confidence: 99%

Assessment of IL‐18 Serum Level and Its Promoter Polymorphisms in the Saudi Coronary Artery Disease (CAD) Patients

Jabir

Firoz

Kamal

et al. 2017

J of Cellular Biochemistry

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The purpose of the current study was to find out the possible changes polymorphic site at the promoter region of IL-18 gene in Saudi CAD patients. We have also measured serum IL-18 level to find out, the likely association between its level and polymorphic site. The present study included total 197 subjects (98 confirmed CAD patients both men and women and 99 healthy control individuals). Serum concentration of IL-18 was measured by enzyme linked immuno-sorbent assay. For SNPs analysis, sanger method of DNA sequencing was followed. We observed variable numbers of SNPs at -137 C/G, -607 A/C, and -656 T/G promoter sites in our studied samples. However, the observed changes in the number of SNP hotspots were found to be non-significant compared with control. IL-18 level was found to be significantly (P < 0.001) elevated in CAD patients compared with control individuals. The highest rise of around 36% (P < 0.001) in IL-18 level was recorded in unstable angina (UA) patients. Moreover, the group belonging to UA and non-ST segment elevation myocardial infarction (NSTEMI) showed only 6% rise. On the basis of our result, inflammation seems to have a role in the pathogenesis of CAD but not leading to the significant changes at the genetic level. J. Cell. Biochem. 118: 1849-1854, 2017. © 2017 Wiley Periodicals, Inc.

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Circulating interleukin-18: A specific biomarker for atherosclerosis-prone patients with metabolic syndrome

Cited by 34 publications

References 51 publications

Differences in Plasma Cytokine Levels between Elite Kayakers and Nonathletes

Differences in Plasma Cytokine Levels between Elite Kayakers and Nonathletes

Effect of essential fatty acids on glucose-induced cytotoxicity to retinal vascular endothelial cells

Assessment of IL‐18 Serum Level and Its Promoter Polymorphisms in the Saudi Coronary Artery Disease (CAD) Patients

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