Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy

Wei, H G; Guan, Meiping; Qin, Yan; Xie, Changchun; Fu, Xia-jun; Gao, Fang; Xue, Yao-ming

doi:10.1507/endocrj.ej14-0246

Cited by 58 publications

(57 citation statements)

References 26 publications

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“…GO is characterized by inflammation of the extraocular muscles and orbital connective tissues, which is caused by the infiltration of lymphocytes and macrophages [2, 3]. The infiltrated lymphocytes and macrophages generate cytokines and lead to the tissue inflammation [4].…”

Section: Introductionmentioning

confidence: 99%

“…MiR-146a, a member of the class of miRNAs, has been found to participate in the pathogenesis of autoimmune diseases such as rheumatoid arthritis (RA), Osteoarthritis (OA), systemic lupus erythematosus (SLE), multiple sclerosis (MS) and GO [3, 9-13]. For instance, MiR-146a was reported to induce TNF-α production by targeting TNF-α/TNF receptor-associated factor 6 (TRAF6)/IL-1 receptor-associated kinase 1 (IRAK1) signaling in RA [14].…”

Section: Introductionmentioning

confidence: 99%

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Mechanism of MicroRNA-146a/Notch2 Signaling Regulating IL-6 in Graves Ophthalmopathy

Wang

Chen

Long

2017

Cell Physiol Biochem

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Background/Aims: We intended to investigate the significance of microRNA-146a, Notch2 and IL-6 on Graves ophthalmopathy (GO) and the relationships among them. Methods: About 27 GO patients were incorporated in this study, including 13 patients with inactive GO and14 patients with active GO. Another 15 patients who had previously received strabismus orthopedics or ophthalmectomy due to trauma were selected as the control population. QRT-PCR assay was used to detect microRNA-146a and Notch2 expression levels in plasma. MTT assay and flow cytometry were respectively used to assess the viability and mitosis of the fibroblasts isolated from orbital connective tissue. Double antibody sandwich enzyme-linked immunosorbent assay (ELISA) was employed to detect serum IL-6 levels. The dual luciferase reporter gene assay was used to verify the targeting relationship between microRNA-146a and Notch2. Results: Compared with the control group, the relative expression of miR-146a was significantly increased whereas the relative expression of Notch2 was significantly decreased (all P < 0.05) in GO patients compare with the control. Notch2 can be directly targeted by microRNA-146a. The over-expression of miR-146a markedly facilitated Orbital Fibroblasts (OFs) viability and mitosis whereas markedly suppressed cell apoptosis (all P < 0.05). Exogenous microRNA-146a mimics could down-regulat the expression of Notch2 and up-regulate IL-6 (P < 0.05). The inhibition of microRNA-146 resulted in the elevated expression of Notch2 and decreased expression of IL-6 (P < 0.05). Conclusion: MicroRNA-146a may increase the IL-6 levels and exacerbate GO by directly targeting Notch2.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mechanism of MicroRNA-146a/Notch2 Signaling Regulating IL-6 in Graves Ophthalmopathy

Wang

Chen

Long

2017

Cell Physiol Biochem

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“…The increase in COX-2 expression by IL-1b was decreased by miR-146a mimics, although the effect was not statistically significantly. Recently, Wei et al 32 reported significantly lower circulating serum levels of miR-146a in GO patients compared with controls, and suggested that weakened miR-146a activity caused an increase in inflammation. The authors further showed that the serum levels of miR-146a were significantly correlated with clinical activity score, which indicates the disease inflammatory activity of GO.…”

Section: Discussionmentioning

confidence: 99%

Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy

Jang

Chae

Lee

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…Graves' ophthalmopathy (GO), also known as thyroid-associated ophthalmopathy, is an organ-specific autoimmune disease (21,22).…”

Section: Graves' Ophthalmopathymentioning

confidence: 99%

99Tc-MDP treatment for the therapy of rheumatoid arthritis, choroidal neovascularisation and Graves' ophthalmopathy

Yang

et al. 2016

Biomedical Reports

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Abstract. Technetium 99 conjugated with methylene diphosphonate, which is an anti-inflammatory drug, can inhibit macrophage infiltration and downregulate a number of proinflammatory cytokines, such as tumor necrosis factor-α and interleukin-1β. Recently, numerous studies have indicated that it could improve rheumatoid arthritis (RA) activity by upregulating the frequency of peripheral γδ T cells and cluster of differentiation CD4 + CD25 + Foxp3 + Tregs, affecting the serum cytokine environment, inhibiting osteoclast formation and reducing the concentrations of rheumatoid factor-immunoglobulin M (IgM)/IgG/IgA. As well, it may have a therapeutic role for choroidal neovascularisation (CNV) and Graves' ophthalmopathy (GO). Therefore, it will be a valuable choice in the treatment of RA, CNV and GO.

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Circulating levels of miR-146a and IL-17 are significantly correlated with the clinical activity of Graves’ ophthalmopathy

Cited by 58 publications

References 26 publications

Mechanism of MicroRNA-146a/Notch2 Signaling Regulating IL-6 in Graves Ophthalmopathy

Mechanism of MicroRNA-146a/Notch2 Signaling Regulating IL-6 in Graves Ophthalmopathy

Role of miR-146a in the Regulation of Inflammation in an In Vitro Model of Graves' Orbitopathy

99Tc-MDP treatment for the therapy of rheumatoid arthritis, choroidal neovascularisation and Graves' ophthalmopathy

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