Circulating microRNAs after a 24‐h ultramarathon run in relation to muscle damage markers in elite athletes

Abstract

Ultra‐endurance sports are growing in popularity but can be associated with adverse health effects, such as exercise‐induced muscle damage (EIMD), which can lead to exertional rhabdomyolysis. Circulating microRNAs (miRNAs) may be useful to approach the degree of EIMD. We aimed to (1) investigate the relevance of circulating miRNAs as biomarkers of muscle damage and (2) examine the acute response of skeletal/cardiac muscle and kidney biomarkers to a 24‐h run in elite athletes. Eleven elite athletes participated in the 24‐h run World Championships. Counter‐movement jump (CMJ), creatine kinase (CK), myoglobin (Mb), creatinine (Cr), high‐sensitive cardiac troponin T (hs‐cTnT), and muscle‐specific miRNA (myomiR) levels were measured before, immediately after, and 24 and 48h after the race. CMJ height was reduced immediately after the race (−84.0 ± 25.2%, p < 0.001) and remained low at 24 h (−43.6 ± 20.4%, p = 0.002). We observed high CK activity (53 239 ± 63 608 U/L, p < 0.001) immediately after the race, and it remained elevated 24h after (p < 0.01). Circulating myomiR levels (miR‐1‐3p, miR‐133a‐3p, miR‐133b, miR‐208a‐3p, miR‐208b‐3p, and miR‐499a‐5p) were elevated immediately after the 24‐h run (fold changes: 18–124,723, p<0.001) and significantly (p < 0.05) correlated or tended to significantly (p < 0.07) correlate with the reduction in CMJ height at 24 h. We found no significant correlation between CMJ height loss at 24 h and CK (p = 0.23) or Mb (p = 0.41) values. All elite ultramarathon runners included in our study were diagnosed with exertional rhabdomyolysis after the 24‐h ultramarathon race. MyomiR levels may be useful to approach the degree of muscle damage.

Acute Supplementation with Cannabidiol Does Not Attenuate Inflammation or Improve Measures of Performance following Strenuous Exercise

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Statin-Associated Myopathy: Emphasis on Mechanisms and Targeted Therapy

“…However, a systematic review of the literature reports conflicting results on the ability of statins to impair performance during exercise [158]. The increase in circulating levels of specific microRNAs (myomiRs) has recently been indicated as a potential biomarker of muscle quality [159]. It has been described that an increase in the levels of some myomiRs is related to intense exertion (miR-1, miR-133a, miR-206), and one in particular (miR-499-5p) seems to increase only in athletes on therapy with statins [160].…”

Dihydroartemisinin ameliorates palmitate-induced apoptosis in cardiomyocytes via regulation on miR-133b/Sirt1 axis

“…In addition, it was revealed that miR-133b played important roles in various diseases. MiR-133b may play a crucial role in suppression genes associated with humans and mice cleft lip (Yoshioka et al, 2021), and act as biomarker in cardiovascular disease (Chang et al, 2021), muscle atrophy (Malacarne et al, 2021), muscle damage (Chalchat et al, 2021). Moreover, lots of reports demonstrated that miR-133b harbored the ability in cardiomyocyte protection in dealing with hypoxia injury (Pan et al, 2018;Zhou et al, 2019) and morphine preconditioning (He et al, 2016).…”