Circulating microRNAs as biomarkers for Sepsis secondary to pneumonia diagnosed via Sepsis 3.0

Abstract

Background Sepsis biomarkers have limited specificity and sensitivity. Few studies have investigated microRNA (miRNA) biomarkers for sepsis secondary to pneumonia. This study aims to investigate the diagnostic and prognostic values of miRNAs in sepsis secondary to pneumonia. Methods Sepsis 3.0 was used to diagnose sepsis. Screening was performed through the Agilent miRNA chip technology by using the following criteria: p < 0.05, fold ≥2 or < 0.5, or copy number > 50 change. This study recruited 52 patients with pneumonia, including 31 males (59.6%) and 21 females (40.4%), 44 patients with sepsis secondary to pneumonia were diagnosed via Sepsis 3.0 (34 [77.3%] males and 10 [22.7%] females), and 21 healthy controls were used for miRNA verification. The miRNA levels were detected through fluorescence real-time quantitative polymerase chain reaction (qRT-PCR). Results: Fluorescence qRT-PCR detection showed that the miR-7110-5p and miR-223-3p expression levels in both patient groups were upregulated compared with those in the healthy controls. The expression levels differed between patients with pneumonia and those with sepsis secondary to pneumonia. The sensitivity and specificity of miR-7110-5p to differentiate sepsis from healthy controls were 84.2 and 90.5%, whereas those of miR-223-3p were 82.9 and 100%, respectively. Multivariate analysis of variance suggested that the presence of sepsis affected the miR-223-3p level ( p = 0.041), whereas the presence of sepsis ( p = 0.000) and the underlying disease ( p = 0.025) influenced the miR-7110-5p level. Conclusions MiR-223-3p could be utilized to predict sepsis secondary to pneumonia.

miRNA-23b as a biomarker of culture-positive neonatal sepsis

“…The sharp indicate significant differences highlighted between all groups using the Kruskal-Wallis test: # p < 0.05 that cause similarities and differences, which influence the patient's situation itself and make sepsis so very complicated (Stearns-Kurosawa et al 2011). This is why the association of miRNAs with sepsis diagnosis remains controversial (Zhang et al 2019b).…”

Accuracy of circulating microRNAs in diagnosis of sepsis: a systematic review and meta-analysis

“…Wu et al [ 22 ] showed miRNA-223 expression was positively correlated with APACHE II score as well. Of note, Wang et al [ 6 ] suggested that miR-223 in serum was downregulated among sepsis patients, while other five studies demonstrated miR-223 in the plasm was upregulated [ 14 – 16 , 22 , 26 ]. In fact, the conflicting results of miR-223 were also reported in hepatitis and hepatocellular carcinoma [ 39 ].…”

miRNA-23b as a biomarker of culture-positive neonatal sepsis

“…One of the main obstacles to establish a well-de ned link between miRNAs and sepsis lies in the fact that sepsis can be caused by very different factors that cause similarities and differences, which in uence the patient's situation itself and make sepsis so very complicated [38]. This is why the association of miRNAs with sepsis diagnosis remains controversial [39]. miRNAs from different biological uids can be used for the early prediction and evaluation of neonatal sepsis, where various miRNAs are down-regulated, and contribute to the initiation of the immune response to infection [40].…”