Circulating MicroRNAs in Graves' Disease in Relation to Clinical Activity

Hayashi, Izumi; Yamada, Hiroya; Munetsuna, Eiji; Hashimoto, Shuji; Itoh, Mitsuyasu

doi:10.1089/thy.2016.0062

Cited by 57 publications

(47 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been reported that the levels of several cytokines such as IL-4, Il-5, IL-6, IL-8, and IL-12 are altered in patients with active GD [25][26][27][28]. Additionally, we previously revealed that the serum profiles of cytokines and chemokines vary depending on disease activity in patients with GD [2,13,29] ered to depend on the cytokine balance during treatment. The changes in IgG4 levels during GD treatment could be attributed to changes in the cytokine balance.…”

Section: Discussionmentioning

confidence: 69%

See 1 more Smart Citation

Changes in Serum Immunoglobulin G4 Levels in Patients with Newly Diagnosed Graves’ Disease

Hayashi

Yamada

Itoh

et al. 2018

Exp Clin Endocrinol Diabetes

Self Cite

View full text Add to dashboard Cite

Objective Immunoglobulin G4 (IgG4)-related disease (IgG4-RD) is an immune-mediated condition that can affect almost any organ. We investigated the association between IgG4-RD and the main characteristics of Graves’ disease (GD) at the time of diagnosis. Additionally, we evaluated whether serum IgG4 levels change during treatment. Design and patients Twenty-eight patients with newly diagnosed GD were enrolled into this longitudinal follow-up study. Serum IgG4 levels and thyroid function were measured in all the participants at the time of diagnosis. Further, the serum IgG4 levels of nine of 28 patients with untreated GD were measured after the achievement of euthyroid state (through the use of methimazole). Results Two (7.1%) of 28 patients with untreated GD had elevated serum IgG4 levels of >135 mg/dL. There was no significant difference in the average IgG4 levels before and after the achievement of euthyroid state (66.2±74.0 mg/dL vs. 50.5±47.3 mg/dL). In two patients, the elevated serum IgG4 levels returned to normal after treatment. However, one patient had an elevated serum IgG4 level of 136.6 mg/dL after treatment. Conclusions This study showed that serum IgG4 levels varied with treatment in patients with GD, independent of thyroid function, suggesting that IgG4 might be indirectly related to GD.

show abstract

Section: Discussionmentioning

confidence: 69%

“…GD is an autoimmune disease characterized by the presence of circulating autoantibodies against the thyroid-stimulating hormone (TSH) receptor. Various factors are involved in GD pathology [1][2][3].…”

Section: Introductionmentioning

confidence: 99%

Changes in Serum Immunoglobulin G4 Levels in Patients with Newly Diagnosed Graves’ Disease

Hayashi

Yamada

Itoh

et al. 2018

Exp Clin Endocrinol Diabetes

Self Cite

View full text Add to dashboard Cite

show abstract

“…Total RNA (2 mg) was reverse-transcribed into cDNA by incubating with deoxynucleoside triphosphate mix (Takara Bio, Kusatsu, Japan), Recombinant RNase Inhibitor (Takara Bio), random primers [hexadeoxyribonucleotide mixture; pd(N)6; Takara Bio], 5X First Strand Buffer (Thermo Fisher Scientific), 100 mM DTT (Thermo Fisher Scientific), and 200 U Moloney Murine Leukemia Virus Reverse Transcriptase (Thermo Fisher Scientific) at 25°C for 10 min, 37°C for 60 min, and then 70°C for 15 min. Finally, the cDNA was used for real-time quantitative PCR (qPCR) reaction (29). qPCR was performed for the quantitative assessment of mRNA expression using an ABI Prism 7900HT Sequence Detection System (Thermo Fisher Scientific) and Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan).…”

Section: Quantitative Assessment Of Mrna Expressionmentioning

confidence: 99%

Maternal fructose–induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi

et al. 2019

Self Cite

View full text Add to dashboard Cite

Fructose consumption is rising globally, but maternal high fructose intake might adversely affect offspring. Our previous report demonstrated that excess maternal fructose intake impairs hippocampal function in offspring, indicating that the hippocampi of offspring are highly sensitive to maternal fructose. Here, we examined the effect of maternal high fructose on mitochondrial physiology and uncoupling protein (UCP) expression. Rat dams received a 20% fructose solution during gestation and lactation. Immediately after weaning, offspring hippocampi were isolated. Maternal high fructose consumption attenuated the mitochondrial O2 consumption rate and stimulated lipid hydroperoxide production in the hippocampi of offspring. Reduced Ucp5 and mitochondrial transcription factor A (Tfam) mRNA levels were also observed after maternal exposure to fructose. We assessed the promoter regions of both genes and found that this treatment enhanced DNA methylation levels. In addition, luciferase assays showed that this DNA methylation could reduce the transcription of both genes. Chromatin immunoprecipitation analysis demonstrated that specificity protein 1 binding to the Ucp5 promoter regions was reduced by DNA methylation. In addition, Ucp5 knockdown induced the up‐regulation of reactive oxygen species levels in a rat brain glioma cell line, whereas reduced O2 consumption was observed with Tfam knockdown. Maternal high fructose intake thus induces reduced O2 oxygen consumption and increases oxidative stress in offspring, at least partly through epigenetic mechanisms involving Ucp5 and Tfam.—Yamada, H., Munetsuna, E., Yamazaki, M., Mizuno, G., Sadamoto, N., Ando, Y., Fujii, R., Shiogama, K., Ishikawa, H., Suzuki, K., Shimono, Y., Ohashi, K., Hashimoto, S. Maternal fructose‐induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi. FASEB J. 33, 11431–11442 (2019). http://www.fasebj.org

show abstract

“…Hiratsuka et al used miRNA array to identify circulating miRNAs in relation to disease activity of GD by recruiting seven intractable GD patients, seven GD patients in remission, and seven healthy controls, and found that miR-23b-5p and miR-92a-3p were significantly increased in GD patients achieving remission, but let-7g-3p and miR-339-5p were significantly lower in GD patients achieving remission than intractable GD patients, demonstrating that some miRNAs could be as biomarkers of intractable GD and treatment response (134). A recent study by Li et al found that patients with higher miR-346 level at diagnosis were at a higher risk of relapse during follow-up (125).…”

Section: Epigenetics In Aitdmentioning

confidence: 99%

The Emerging Role of Epigenetics in Autoimmune Thyroid Diseases

et al. 2017

View full text Add to dashboard Cite

Autoimmune thyroid diseases (AITD) are a group of both B cell- and T cell-mediated organ-specific autoimmune diseases. Graves’ disease and Hashimoto thyroiditis are the two main clinical presentations of AITD. Both genetic and environmental factors have important roles in the development of AITD. Epigenetics have been considered to exert key roles in integrating those genetic and environmental factors, and epigenetic modifications caused by environmental factors may drive genetically susceptibility individuals to develop AITD. Recent studies on the epigenetics of AITD have provided some novel insights into the pathogenesis of AITD. The aim of this review is to provide an overview of recent advances in the epigenetic mechanisms of AITD, such as DNA methylation, histone modifications, and non-coding RNAs. This review highlights the key roles of epigenetics in the pathogenesis of AITD and potential clinical utility. However, the epigenetic roles in AITD are still not fully elucidated, and more researches are needed to provide further deeper insights into the roles of epigenetics in AITD and to uncover new therapeutic targets. Although there are many studies assessing the epigenetic modifications in AITD patients, the clinical utility of epigenetics in AITD remains poorly defined. More studies are needed to identify the underlying epigenetic modifications that can contribute to accurate diagnosis of AITD, adequate choice of treatment approach, and precise prediction of treatment outcomes.

show abstract

Circulating MicroRNAs in Graves' Disease in Relation to Clinical Activity

Abstract: This study demonstrates that different levels of circulating miRNAs are associated with intractable GD. Moreover, serum exosomes of patients with intractable GD may activate immune cells, which may play an important role in GD pathogenesis.

Cited by 57 publications

References 60 publications

Changes in Serum Immunoglobulin G4 Levels in Patients with Newly Diagnosed Graves’ Disease

Changes in Serum Immunoglobulin G4 Levels in Patients with Newly Diagnosed Graves’ Disease

Maternal fructose–induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi

The Emerging Role of Epigenetics in Autoimmune Thyroid Diseases

Contact Info

Product

Resources

About