Circulating nucleic acids as liquid biopsies for disease prediction, screening and diagnosis

Sun, Yuqing; Tang, Yongling; Yu, Long; Wang, Yuhao; Peng, Shuang; Zhou, Xiang

doi:10.1007/s11426-023-1727-9

Cited by 4 publications

(2 citation statements)

References 124 publications

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“…POCT not only hold immense significance in early disease detection and prevention, but also offer enhanced convenience for home and community hospitals. [36,37] Herein, we employed a compact and user-friendly fluorescence detection equipment (Figure 5E; Figure S10) to evaluate the performance of QM-N2 in diagnosing mALB-related diseases. Remarkably, the results demonstrated the high sensitivity of QM-N2 and an approximately linear correlation between ΔF and mALB (Figure 5F,G; Figure S11), akin to the relationship depicted in Figure 5B,C, confirming the exceptional selectivity of QM-N2 in diagnosing mALB related diseases (Figure 5H) with high accuracy (Table S1).…”

Section: Point-of-care Diagnosis Of Malb-related Diseases With Portab...mentioning

confidence: 99%

Microalbuminuria sensitive near‐infrared AIE probe for point‐of‐care evaluating kidney diseases

Zhu,

Chen,

Liao

et al. 2024

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Urinary microalbumin (mALB) serves as an exceptionally sensitive indicator for the early detection of kidney damage, playing a pivotal role in identifying chronic renal failure and kidney lesions in individuals. Nevertheless, the current fluorescent methodologies for point‐of‐care (POC) diagnosis of mALB in real urine still exhibit suboptimal performance. Herein, the development and synthesis of QM‐N2, an albumin‐activated near‐infrared (NIR) aggregation‐induced emission (AIE) fluorescent probe, are presented. The strategic incorporation and positioning of quaternary ammonium salts within the quinoline‐malononitrile (QM) scaffold significantly influence solubility and luminescence characteristics. Specifically, the quaternary ammonium salt‐free variant, QM‐OH, and the quaternary ammonium salt integrated at the donor function group (DFG) site, QM‐N1, display limited solubility in aqueous solutions while demonstrating a distinct fluorescence signal. Conversely, the incorporation of quaternary ammonium salt at the conformational functional group (CFG) site in QM‐N2 imparts superior dispersibility in water and reduces the initial fluorescence. Furthermore, the integration of a well‐defined D‐π‐A structure within QM‐N2 enables itself with near‐infrared emission, which is crucial for mitigating interference from autofluorescence present in urine samples. Upon interaction with albumin, QM‐N2 forms a tight bond with the IIA site of the subdomain of human serum albumin (HSA), inducing alterations in protein configuration and constraining the intrinsic motion of fluorescent molecules. This interaction induces fluorescence, facilitating the sensitive detection of trace albumin. Ultimately, QM‐N2 is applied for POC testing of mALB using portable equipment, particularly in the diagnosis of mALB‐related diseases, notably chronic renal failure. This positioning underscores its potential as an ideal candidate for self‐health measurement at home or in community hospitals.

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Section: Point-of-care Diagnosis Of Malb-related Diseases With Portab...mentioning

confidence: 99%

Microalbuminuria sensitive near‐infrared AIE probe for point‐of‐care evaluating kidney diseases

Zhu,

Chen,

Liao

et al. 2024

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“…They are critical regulators of gene expression for numerous diseases. Researchers have shown that miRNAs can be released or leaked from cancer cells in the form of microvesicles, exosomes, or protein complexes and circulate in various body fluids in extremely stable forms . Moreover, there is a significant correlation between circulating miRNA levels and cancer progression.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Method Enables Liquid Biopsy for the Precise Detection of Circulating MicroRNAs

Chen,

Zhang,

Xiao

et al. 2024

Anal. Chem.

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Due to invasive and serial examinations of bioactive molecules, liquid biopsy (LB) has emerged as a rapid and reliable solution for early disease detection and monitoring. Developing portable devices with high specificity and sensitivity for LB is highly valuable. To realize a generalized approach to increase the sensitivity of LB, we developed an ultrasensitive diagnostic biochip based on the amplification of miRNA by recombinase polymerase amplification and the significant enhancement of fluorescence signals by photonic crystal (PC) materials. The PCs-RPA biochip has a detection limit as low as 0.24 aM, a wide linear range of 8 orders of magnitude, and excellent specificity. Such advantages realize the accurate detection of circulating miRNAs with very low content in clinical serum samples for the precise diagnosis of nonsmall cell lung cancer.

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Advanced luminescent metal–organic framework (MOF) sensors engineered for urine analysis applications

Mohan,

Virender,

Kumar Gupta

et al. 2024

Coordination Chemistry Reviews

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Circulating nucleic acids as liquid biopsies for disease prediction, screening and diagnosis

Cited by 4 publications

References 124 publications

Microalbuminuria sensitive near‐infrared AIE probe for point‐of‐care evaluating kidney diseases

Microalbuminuria sensitive near‐infrared AIE probe for point‐of‐care evaluating kidney diseases

Ultrasensitive Method Enables Liquid Biopsy for the Precise Detection of Circulating MicroRNAs

Advanced luminescent metal–organic framework (MOF) sensors engineered for urine analysis applications

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