Circulating Pre-microRNA-488 in Peripheral Blood Is a Potential Biomarker for Predicting Recurrence in Breast Cancer

Masuda, Takaaki; Shinden, Yoshiaki; Noda, Manjiro; Ueo, Hiroki; Hu, Qingjiang; Yoshikawa, Yutaka; Tsuruda, Yusuke; Kuroda, Yasuhisa; Ito, Shuhei; Eguchi, Hidetoshi; Ohno, Shinji; Mimori, Koshi

doi:10.21873/anticanres.12755

Cited by 28 publications

(24 citation statements)

References 18 publications

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“…. RT was performed using M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA, USA), as previously described (23,24), and the quantitative polymerase chain reaction (qPCR) was performed using a LightCycler 480 SYBR Green Ⅰ Master Mix (Roche, Basel, Switzerland) as previously described (23,24). The following primers were used: KIF15: 5'-AATTTGCGGCCATCTTGAGC-3' (sense) and 5'-CTGACCATTTGTCACGCTGC-3' (antisense); GAPDH: 5'-AGCCACATCGCTCAGACAC-3' (sense) and 5'-GCC CAATACG ACCAAATCC-3' (antisense).…”

Section: Reverse Transcription-quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma

Kitagawa

Masuda

Takahashi

et al. 2020

Cancer Genomics Proteomics

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Background/Aim: Kinesin family member 15 (KIF15) participates in the transport of macromolecules in essential cellular processes. In this study we evaluated the clinical relevance of KIF15 expression in hepatocellular carcinoma (HCC). Materials and Methods: Association between KIF15 expression and clinical outcomes in HCC patients was analyzed using three independent cohorts. Localization of KIF15 expression was assessed by immunohistochemical analysis. Co-culture experiments were performed using healthy donor peripheral blood mononuclear cells (PBMC) and HCC cell lines. Results: Immunohistochemical analysis showed that KIF15 was mainly expressed in inflammatory monocytes around cancer cells. Multivariate analysis indicated high KIF15 expression was an independent poor prognostic factor for survival. HCC cells with high expression of minichromosome maintenance protein 2 (MCM2) were located close to KIF15expressing inflammatory monocytes. The proliferation ability of HCC cells was increased by co-culture with PBMC. Conclusion: High KIF15 expression in inflammatory monocytes in tumor tissues may serve as a prognostic marker for poor outcome in HCC.

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Section: Reverse Transcription-quantitative Pcr (Rt-qpcr)mentioning

confidence: 99%

KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma

Kitagawa

Masuda

Takahashi

et al. 2020

Cancer Genomics Proteomics

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“…Total RNA was isolated from the frozen tissue specimens through use of ISOGEN (Nippon Gene, Tokyo, Japan), according to the manufacturer's protocol. Complementary DNA (cDNA) was synthesized from 8 μg of total RNA using M-MLV reverse transcriptase (Invitrogen, Carlsbad, CA, USA) as previously described (15). qPCR was performed using a LightCycler 480 and a SYBR Green I Master kit (Roche Applied Science, Basel, Switzerland) following the manufacturer's protocol as previously described (16).…”

Section: Rna Extraction and Reverse Transcription-quantitative Polymementioning

confidence: 99%

Cytolytic Activity (CYT) Score Is a Prognostic Biomarker Reflecting Host Immune Status in Hepatocellular Carcinoma (HCC)

et al. 2018

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“…To determine the clinical significance of preoperative gene expression in the PB of patients with primary breast cancer undergoing curative surgery, we used clinical data from breast cancer patients described previously (Kyushu cohort) [35]. Briefly, a total of 594 patients with breast cancer underwent primary tumor resection at the Department of Breast Oncology, National Kyushu Cancer Center (Fukuoka, Japan), from 2000 to 2008.…”

Section: Clinical Samplesmentioning

confidence: 99%

“…We used Polymorphprep TM (Alere Technologies AS, Oslo, Norway) to isolate three blood cell fractions (PMNs, MCs, and RBCs) according to the manufacturer's instructions, as described previously [35]. Each fraction was mixed with ISOGEN II (Nippon Gene) and stored at −80 • C until RNA extraction.…”

Section: Separation Of Three Blood Cell Fractionsmentioning

confidence: 99%

N-Cadherin mRNA Levels in Peripheral Blood Could Be a Potential Indicator of New Metastases in Breast Cancer: A Pilot Study

Masuda

Ueo²,

Kai

et al. 2020

IJMS

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Background: There is growing evidence that patients with metastatic breast cancer whose disease progresses from a new metastasis (NM) have a worse prognosis than that of patients whose disease progresses from a pre-existing metastasis. The aim of this pilot study is to identify a blood biomarker predicting NM in breast cancer. Methods: The expression of epithelial (cytokeratin 18/19) or mesenchymal (plastin-3, vimentin, and N-cadherin) markers in the peripheral blood (PB) of recurrent breast cancer patients undergoing chemotherapy with eribulin or S-1 was measured over the course of treatment by RT-qPCR. The clinical significance of preoperative N-cadherin expression in the PB or tumor tissues of breast cancer patients undergoing curative surgery was assessed by RT-qPCR or using public datasets. Finally, N-cadherin expression in specific PB cell types was assessed by RT-qPCR. Results: The expression levels of the mesenchymal markers N-cadherin and vimentin were high in the NM cases, whereas that of the epithelial marker cytokeratin 18 was high in the pre-existing metastasis cases. High preoperative N-cadherin expression in PB or tumor tissues was significantly associated with poor recurrence-free survival. N-cadherin was expressed mainly in polymorphonuclear leukocytes in PB. Conclusion: N-cadherin mRNA levels in blood may serve as a novel prognostic biomarker predicting NM, including recurrence, in breast cancer patients.

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Circulating Pre-microRNA-488 in Peripheral Blood Is a Potential Biomarker for Predicting Recurrence in Breast Cancer

Abstract: Circulating pre-miR-488 expression could be a novel prognostic biomarker for predicting recurrence in breast cancer patients.

Cited by 28 publications

References 18 publications

KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma

KIF15 Expression in Tumor-associated Monocytes Is a Prognostic Biomarker in Hepatocellular Carcinoma

Cytolytic Activity (CYT) Score Is a Prognostic Biomarker Reflecting Host Immune Status in Hepatocellular Carcinoma (HCC)

N-Cadherin mRNA Levels in Peripheral Blood Could Be a Potential Indicator of New Metastases in Breast Cancer: A Pilot Study

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