Circulating proteasome activity following mild head injury in children

Tylicka, Marzena; Matuszczak, Ewa; Dębek, Wojciech; Hermanowicz, Adam; Ostrowska, H

doi:10.1007/s00381-014-2409-4

Cited by 18 publications

(9 citation statements)

References 37 publications

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“…In our study we did not find a correlation of immunoproteasome, 20S proteasome and UCHL1 levels with gender or age, which is consistent with previous observations [9][10][11].…”

Section: Discussionsupporting

confidence: 82%

“…The PA700 regulator binds to the outer a-rings of the 20S core and, also according to Ostrowska et al [4], the 'resulting 26S proteasome is responsible for energy-dependent degradation of polyubiquitinated proteins, including many short-lived regulatory proteins that control cell cycle progression, apoptosis, signal transduction, and gene expression' [5]. In humans, extracellular proteasomes have been found circulating in the plasma of patients suffering from a wide range of inflammatory autoimmune diseases and with neoplasms [6][7][8][9][10][11]. It is already known that the level of proteasomes correlate with the severity of the disease in different pathologies [12].…”

Section: Introductionmentioning

confidence: 99%

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Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Matuszczak

Sankiewicz

Dębek

et al. 2017

Clinical and Experimental Immunology

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SummaryThe aim of this study was to determinate the immunoproteasome concentration in the blood plasma of children with appendicitis, and its correlation with circulating proteasome and ubiquitin carboxyl-terminal hydrolase L1 (UCHL1). Twenty-seven children with acute appendicitis, managed at the Paediatric Surgery Department, were included randomly into the study (age 2 years 9 months up to 14 years, mean age 9Á5 6 1 years). There were 10 girls and 17 boys; 18 healthy, age-matched subjects, admitted for planned surgeries served as controls. Mean concentrations of immunoproteasome, 20S proteasome and UCHL1 in the blood plasma of children with appendicitis before surgery 24 h and 72 h after the appendectomy were higher than in the control group. The immunoproteasome, 20S proteasome and UCHL1 concentrations in the blood plasma of patients with acute appendicitis were highest before surgery. The immunoproteasome, 20S proteasome and UCHL1 concentration measured 24 and 72 h after the operation decreased slowly over time and still did not reach the normal range (P < 0Á05). There was no statistical difference between immunoproteasome, 20S proteasome and UCHL1 concentrations in children operated on laparoscopically and children after classic appendectomy. The immunoproteasome concentration may reflect the metabolic response to acute state inflammation, and the process of gradual ebbing of the inflammation may thus be helpful in the assessment of the efficacy of treatment. The method of operation -classic open appendectomy or laparoscopic appendectomy -does not influence the general trend in immunoproteasome concentration in children with appendicitis.

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“…In our study we did not find a correlation of immunoproteasome, 20S proteasome and UCHL1 levels with gender or age, which is consistent with previous observations [9][10][11].…”

Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Matuszczak

Sankiewicz

Dębek

et al. 2017

Clinical and Experimental Immunology

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“…Several lines of evidence strongly suggested that the acute-phase response is not diagnostic for any particular disease, but occurs as a response to several pathological conditions and diseases [ 33 ]. Our earlier findings showed that the proteasome may be a biomarker of tissue damage—more severe in the group of burn patients in comparison to patients with mild head injury and blunt abdominal trauma [ 34 , 35 ]. Also, the last study showed that correlation between proteasome activity and length of surgery in contrast to the C-reactive protein indicated that CRP is only an indicator of the pathological state, while the function of the proteasome is more complex due to its participation in the oxidative stress-induced repair of tissue damage [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Proteasome Activity and C-Reactive Protein Concentration in the Course of Inflammatory Reaction in Relation to the Type of Abdominal Operation and the Surgical Technique Used

Tylicka

Matuszczak

Karpińska

et al. 2018

Mediators of Inflammation

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Surgical tissue damage and the accompanying inflammatory response lead to proteasome activation, initiation of damaged protein degradation, and induction of acute-phase inflammatory response. The aim of this study was to investigate the rate of change in proteasome chymotrypsin-like (ChT-L) activity and C-reactive protein concentration depending on the degree of tissue damage and their correlation with prealbumin concentrations in children before and after abdominal surgery. This experimental study included children who underwent abdominal surgery between 2015 and 2017. Plasma prealbumin concentrations and C-reactive protein levels (CRP) were determined by standard biochemical laboratory procedures. Proteasome activity was assessed using a Suc-Leu-Leu-Val-Tyr-AMC peptide substrate. Elevation of plasma proteasome activity was noted in children after laparoscopic and open abdominal surgeries. However, 20S proteasome activity in children undergoing conventional open surgery was significantly higher (P < 0.05) than in patients subjected to laparoscopy. At the same time, an increase in the CRP level was observed. However, there was no correlation between C-reactive protein concentrations and the type of abdominal surgery while there was a correlation observed in the case of proteasomes. Proteasome activity correlates with the degree of surgical tissue damage and prealbumin concentrations. More invasive surgery leads to a stronger activation of the proteasome involved in removing proteins that were damaged due to the surgical procedure. Proteasomes are more specific markers because there is a correlation between proteasome activity and the type of abdominal surgery in contrast to C-reactive protein concentrations which are not different in response to surgery performed in regard to ovarian cysts or cholelithiasis.

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“…In a similar study conducted by Oldziej et al 59 , both the concentration and the activity of c-proteasomes in plasma samples were shown to be significantly higher in patients with multiple myeloma (4.38 μg/mL and 1.32 U/mg [ N = 64]) than in healthy controls (2.01 μg/mL and 1.02 U/mg [ N = 30]). The Matuszczak group analyzed the plasma levels of c-proteasomes in pediatric patients with mild head injury 60 , acute appendectomy 61 , and moderate to major burns 62 . In general, the group found that c-proteasome activity and concentration increased initially following an acute onset and reduced gradually after treatment.…”

Section: Circulating Proteasomesmentioning

confidence: 99%

Concept and application of circulating proteasomes

et al. 2021

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Proteostasis is primarily a function of protein synthesis and degradation. Although the components and processes involved in intracellular proteostasis have been studied extensively, it is apparent that extracellular proteostasis is equitably crucial for the viability of organisms. The 26S proteasome, a unique ATP-dependent proteolytic complex in eukaryotic cells, contributes to the majority of intracellular proteolysis. Accumulating evidence suggests the presence of intact 20S proteasomes in the circulatory system (c-proteasomes), and similar to other plasma proteins, the levels of these c-proteasomes may vary, potentially reflecting specific pathophysiological conditions. Under normal conditions, the concentration of c-proteasomes has been reported to be in the range of ~0.2–2 μg/mL, which is ~2–4-fold lower than that of functional plasma proteins but markedly higher than that of signaling proteins. The characterization of c-proteasomes, such as their origin, structure, role, and clearance, has been delayed mainly due to technical limitations. In this review, we summarize the current perspectives pertaining to c-proteasomes, focusing on the methodology, including our experimental understanding. We believe that once the pathological relevance of c-proteasomes is revealed, these unique components may be utilized in the diagnosis and prognosis of diverse human diseases.

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Circulating proteasome activity following mild head injury in children

Cited by 18 publications

References 37 publications

Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Proteasome Activity and C-Reactive Protein Concentration in the Course of Inflammatory Reaction in Relation to the Type of Abdominal Operation and the Surgical Technique Used

Concept and application of circulating proteasomes

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