Immunoproteasome in the blood plasma of children with acute appendicitis, and its correlation with proteasome and UCHL1 measured by SPR imaging biosensors

Abstract

SummaryThe aim of this study was to determinate the immunoproteasome concentration in the blood plasma of children with appendicitis, and its correlation with circulating proteasome and ubiquitin carboxyl-terminal hydrolase L1 (UCHL1). Twenty-seven children with acute appendicitis, managed at the Paediatric Surgery Department, were included randomly into the study (age 2 years 9 months up to 14 years, mean age 9Á5 6 1 years). There were 10 girls and 17 boys; 18 healthy, age-matched subjects, admitted for planned surgeries served as controls. Mean concentrations of immunoproteasome, 20S proteasome and UCHL1 in the blood plasma of children with appendicitis before surgery 24 h and 72 h after the appendectomy were higher than in the control group. The immunoproteasome, 20S proteasome and UCHL1 concentrations in the blood plasma of patients with acute appendicitis were highest before surgery. The immunoproteasome, 20S proteasome and UCHL1 concentration measured 24 and 72 h after the operation decreased slowly over time and still did not reach the normal range (P < 0Á05). There was no statistical difference between immunoproteasome, 20S proteasome and UCHL1 concentrations in children operated on laparoscopically and children after classic appendectomy. The immunoproteasome concentration may reflect the metabolic response to acute state inflammation, and the process of gradual ebbing of the inflammation may thus be helpful in the assessment of the efficacy of treatment. The method of operation -classic open appendectomy or laparoscopic appendectomy -does not influence the general trend in immunoproteasome concentration in children with appendicitis.

Systematic review and meta-analysis of the effects of the perioperative enhanced recovery after surgery concept on the surgical treatment of acute appendicitis in children

“…Acute appendicitis in children is a common acute abdomen, and because of the characteristics of pediatric anatomy and physiology, the disease development is very rapid, and can easily lead to the formation of appendiceal gangrene, perforation, and abscess (25). The disease is generally treated clinically by laparoscopic appendectomy.…”

Recent Progress in Surface Plasmon Resonance Biosensors (2016 to Mid-2018)

“…Papers pertaining to stage (v) [28,29,30,31,32,33,34,35,36,37] represent completely developed analytical procedures that have been applied in various clinical investigations. An example is a paper reporting the determination of three markers, laminin-5, collagen IV, and matrix metalloproteinase-2, in the blood plasma of pediatric burn patients [32].…”

A Comparison of Various Chips Used for the Manufacture of Biosensors Applied in Non-Fluidic Array SPRi, Based on the Example of Determination of Cathepsin D

“…Most of these are described, among other biosensors, in two review papers [ 5 , 6 ]. The majority of these biosensors have been used in clinical investigations, including studies concerning bladder cancer [ 7 , 8 , 9 ], endometriosis [ 6 , 7 , 8 , 9 , 10 ], burns healing [ 11 ], acute appendicitis [ 12 ], and cryptorchidism [ 13 ]. In contrast to the fluidic SPR technique, these biosensors are suitable for the determination of a required marker without signal enhancement or preliminary preconcentration, which significantly facilitates the determination of a biomarker in a body fluid such as human serum.…”