Circulating Reticulocytes in Normal Adults as Determined by the New Methylene Blue Method

Deiss, Andrew; Kurth, Doris

doi:10.1093/ajcp/53.4.481

Cited by 37 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Comparison of the normal range data for the reticulocyte concentration using the TT method in both adults and newborns demonstrates values which are consistent with those reported with manual reticulocyte counting tech niques [13,14], Collectively, our adult nor mal range studies carried out with samples from 811 blood bank donors using the TT, EB, or TO methods have yielded comparable data and a well-defined normal range. How ever, it is important to note that the fluores cent technique generally yields reticulocyte levels which are slightly greater than those observed with the manual technique.…”

Section: Discussionsupporting

confidence: 85%

Enumeration of Reticulocytes Using Fluorescence-Activated Flow Cytometry

Corash¹,

Rheinschmidt²,

Lieu³

et al. 1988

Path Immunopathol Res

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 85%

Enumeration of Reticulocytes Using Fluorescence-Activated Flow Cytometry

Corash¹,

Rheinschmidt²,

Lieu³

et al. 1988

Path Immunopathol Res

View full text Add to dashboard Cite

“…The enzyme activity measurements and hematocrit determinations were made within 2 h of blood sampling. Microscopic determination of reticu locyte counts was done on each occasion of blood letting using a methylene blue stain (10,12). ALA-D activity measurements were done on seven occasions after the injection (7th, 8th, 9th, 10th, 12th, 13th and Results Figure 1 shows the ALA-D activity in the red blood cells of the experimental animals as well as the controls.…”

Section: Methodsmentioning

confidence: 99%

Effect of Phenylhydrazine on Delta-Aminolevulinic Acid Dehydratase in Red Blood Cells

Abdulla¹,

Svensson²

1978

Enzyme

View full text Add to dashboard Cite

δ-aminolevulinic acid dehydratase activity was measured in the blood of rats poisoned by successive injections of phenylhydrazine (1 ml, 35 mmol). After 5 days of successive injections, more than 90% of the circulating blood cells consisted of reticulocytes and the enzyme activity also increased successively and reached a maximum corresponding to the number of reticulocytes. Thus ô-aminolevulinic acid dehydratase activity measured in the peripheral blood is mainly due to the percentage of circulating reticulocytes.

show abstract

“…Inoculation with PHZ is reported to cause hemolytic anemia, whereby PHZ-treated animals can be used as a model for anemia (Jain and Hochstein, 1980;LatundeDada et al, 2006). Reticulocytes are immature RBCs that increase when erythropoiesis is promoted during anemia (Deiss and Kurth, 1970;Jain and Hochstein, 1980). We show that the time until a significant decrease of PCV happened was different between ILS-infected and PHZ-treated rats.…”

Section: Discussionmentioning

confidence: 77%

“…The packed cell volume (PCV) of all rats was measured after centrifugation. The percentage of reticurocytes was measured by new methylene blue method (Deiss and Kurth, 1970). In this study, rats that had a PCV that was significantly lower than the PCV of uninfected rats were considered to have anemia.…”

Section: Animalsmentioning

confidence: 99%

Effect of Trypanosoma brucei brucei on Erythropoiesis in Infected Rats

Nishimura¹,

Nakaya²,

Nakagawa³

et al. 2011

Journal of Parasitology

View full text Add to dashboard Cite

Anemia generated from African trypanosome infection is considered an important symptom in humans and in domestic animals. In order to recover from anemia, the process of erythropoiesis is essential. Erythropoiesis is affected by erythropoietin (EPO), an erythropoietic hormone, supplying iron and inflammatory and proinflammatory cytokines. However, the role of these factors in erythropoiesis during African trypanosome infection remains unclear. In the present study, we analyze how erythropoiesis is altered in anemic Trypanosoma brucei brucei (interleukin-tat 1.4 strain [ILS])-infected rats. We report that the packed cell volume (PCV) of blood from ILS-infected rats was significantly lower 4 days after infection, whereas the number of reticulocytes, as an index of erythropoiesis, did not increase. The level of EPO mRNA in ILS-infected rats did not increase from the third day to the sixth day after infection, the same time that the PCV decreased. Kidney cells of uninfected rats cultured with ILS trypanosome strain for 8 hr in vitro decreased EPO mRNA levels. Treatment of both ILS and cobalt chloride mimicked hypoxia, which restrained the EPO-production-promoting effect of the cobalt. Messenger RNA levels of β-globin and transferrin receptor, as markers of erythropoiesis in the bone marrow, also decreased in ILS-infected rats. Levels of hepcidin mRNA, which controls the supply of iron to the marrow in liver, were increased in ILS-infected rats; however, the concentration of serum iron did not change. Furthermore, mRNA levels of interleukin-12, interferon-γ, tumor necrosis factor-α, and macrophage migration inhibitory factor in the spleen, factors that have the potential to restrain erythropoiesis in bone marrow, were elevated in the ILS-infected rats. These results suggest that ILS infection in rats affect erythropoiesis, which responds by decreasing EPO production and restraining its function in the bone marrow.

show abstract

Circulating Reticulocytes in Normal Adults as Determined by the New Methylene Blue Method

Cited by 37 publications

References 0 publications

Enumeration of Reticulocytes Using Fluorescence-Activated Flow Cytometry

Enumeration of Reticulocytes Using Fluorescence-Activated Flow Cytometry

Effect of Phenylhydrazine on Delta-Aminolevulinic Acid Dehydratase in Red Blood Cells

Effect of Trypanosoma brucei brucei on Erythropoiesis in Infected Rats

Contact Info

Product

Resources

About