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in doses up to 20 mg dissolved in Locke-Ringer solution and injected into the coronary inflow cannula produced only slight. transient depression of contractions and some reduction of rate.Concentrations of panganiic acid up to 6 X 10r4 M did not alter spontaneous contractions of the isolated rabbit ileum. nor did this concentration of drug modify contractions produced by acetylcholine. Our data demonstrate that pangamic acid is not without pharmacologic effect. 11-e have found the LD-50 in mice after intraperitoneal administration to be much lower than the value previously reported for this compound. It has been reported that thiamine hydrochloride possesses neuromuscular blocking activity and that this effect is antagonized by neostigmine methylsulfate(4.5. 6 ) . I t has also been demonstrated that intravenous administration of thiamine hydrochloride produced a transient but marked hypotension with concomitant bradycardia ir! anesthetized dogs. In these respects, pangamic acid produces pharmacological effects not unlike those of thiamine hydrochloride. Circulatory collapse has been reported clinically from intravenous and intramuscular injections of thiamine hydrochloride ( 10). 11-e are not aware of any clinical reports of respiratory depression or severe hypotension resul t ing from administration of pangamic acid. However, it is conceivable that pan-gamic acid could produce undesirable clinical effects under circumstances of overdosage. Sumnz~ry. Pangamic acid (Vit. B-15)possesses definite pharmacologic properties. These include neuromuscular blocking activity in the rabbit and chicken as well as production of hypotension in anesthetized dogs. Neostigmine methylsulfate is an effective antagonist to the neuromuscular blockade produced in the rabbit. These effects appear to be qualitatively similar to those reported for thiamine hydrochloride.The use of radioactive diisopropylfluoro-this laboratory( 1-3). Leukocytes have been phosphate (DFPA2) as a label for leukocytes labeled in vitro and returned to the circulahas been described in previous reports from tion of the donor(2), or they have been la-*This investigation was supported in part by a beled in vivo by administering the DFP32 inresearch grant from Nat. Inst. of Arthritis and travenously ( 1,s ) -During these studies it Metab. Dis., Nat. Inst. Health, U.S.P.H.S. and in was demonstrated that pdymorphonuclear part by U. S. Atomic Energy Commission. (PMN) neutrophils but not lymphocytes are by guest on July 27, 2015 ebm.sagepub.com Downloaded from LEUKOCYTE UPTAKE OF TRITIATED DFP 423 labeled by DFPJ2. It was not possible to determine whether eosinophils, basophils and monocytes are labeled.When leukocytes were labeled in vivo following intravenous administration of DFP32, the blood leukocyte radioactivity curve obtained was complex( 1,3). The shape of the curve suggested that granulocytes in the bone marrow as well as in the circulation were labeled, but that the circulating granulocytes, due to exposure to a higher initial concentration of D F P 2 , were ...

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The number of circulating erythrocytes containing reticulum and no siderotic granules (R cells), both reticulum and siderotic granules (R-S cells), and siderotic granules and no reticulum (S cells) was determined in 80 normal adults, 7 normal newborns and 486 patients with hematologic disorders. Few R-S cells (maximum 0.3 per cent) or S cells (maximum 0.2 per cent) were observed in the blood of normal adults. The number of siderocytes as observed in the direct Prussian blue method (direct-total siderocytes) did not exceed 0.7 per cent. R-S cells greater than 1.0 per cent and direct-total siderocytes greater than 2.0 per cent were observed in each of 7 normal newborns. Among the 486 patients, R-S cells more numerous than 1.0 per cent, S cells greater than 1.0 per cent, or direct-total siderocytes greater than 2.0 per cent were observed only in four conditions. Increases in R-S cells were seen in patients with severe hemolytic anemia and in patients with sideroblastic anemia associated with mild hemolysis. Increases in S cells were seen in splenectomized patients in the absence of hemolysis. Increases both in R-S cells and S cells were seen in patients with splenic atrophy or replacement and in splenectomized patients in the presence of hemolysis.

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