CircZNF609 promotes breast cancer cell growth, migration, and invasion by elevating p70S6K1 via sponging miR-145-5p

Wang, Shengting; Xue, Xiaomei; Wang, Rui; Li, Xiaoming; Li, Qian; Wang, Yufang; Xie, Pei-Jun; Kang, Yu-hua; Meng, Rui; Feng, Xinghua

doi:10.2147/cmar.s174778

Cited by 69 publications

(51 citation statements)

References 33 publications

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“…This hypothesis was confirmed in the following, as the cytoprotective impacts of cZNF609 silence towards HaCaT cells were alleviated when miR-145 level was suppressed by the specific inhibitor. Previously, cZNF609 has been found to be the molecular sponges of a small fraction of miRNAs, such as miR-615 [33], miR-138-5p [17], miR-150-5p [34] and miR-145 [35]. Our findings agree with the previous observations.…”

Section: Discussionsupporting

confidence: 93%

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Gao

2020

Artificial Cells, Nanomedicine, and Biotechnology

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Background: CircZNF609 (cZNF609) is previously revealed as an essential mediator in oxidative stress. This paper determined the role of cZNF609 in skin oxidative damage to evaluate its importance in pressure ulcer. Methods: HaCaT cells treated by H 2 O 2 were considered as a cell model of pressure ulcer. The role of cZNF609 in the model was checked by conducting CCK-8 assay, FITC-PI double-staining, ROS detection and Western blot. The downstream gene and signalling of cZNF609 were studied by utilizing qRT-PCR and Western blot. Results: HaCaT cells were remarkably damaged by H 2 O 2 , as evidenced by the viability loss, apoptosis and ROS generation. It was coupled with the elevated expression of p53, p16, Bax and the activated forms of caspase-3 and PARP. Meanwhile, cZNF609 was high-expressed in response to H 2 O 2. The oxidative stress driven by H 2 O 2 was alleviated by transfection with cZNF609 specific siRNA. Further, the antiantioxidant impacts of cZNF609 silence were impeded by miR-145 silence. The inhibition of JNK and p38MAPK pathways induced by cZNF609 silence was impeded by miR-145 silence. Conclusion: The protective function of cZNF609 silence in H 2 O 2-injured HaCaT cells was revealed in vitro. Silence of cZNF609 exhibited its impact possibly through regulating miR-145, and JNK and p38MAPK pathways.

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Section: Discussionsupporting

confidence: 93%

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Gao

2020

Artificial Cells, Nanomedicine, and Biotechnology

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“…For instance, there was a study that pointed out that circZNF609 enhanced the progress of nasopharyngeal tumour [10]. Besides, other study pointed out that circZNF609 enhanced breast tumour [19].…”

Section: Discussionmentioning

confidence: 99%

Silencing circular RNA circZNF609 restrains growth, migration and invasion by up-regulating microRNA-186-5p in prostate cancer

Jin

Zhao

Zhang

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Background: Prostate cancer (PC) fearfully impacts men's health. We explored the efficacy and mechanism of circular RNA circZNF609 (circZNF609) on colony formation, viability, apoptosis, migration and invasion and in PC cells. Methods: Colony formation, CCK-8, flow cytometry, migration and invasion assay were respectively used to detect the functions of circZNF609 and microRNA (miR)-186-5p on cell colony ability, viability, apoptosis, migration and invasion. circZNF609 and miR-186-5p expression were changed by cell transfection and tested by RT-qPCR. Moreover, Cleaved-Caspase-3, Cleaved-Caspase-9, matrix metalloproteinase-9 (MMP-9), Vimentin and relate-proteins of cell pathways were examined through Western blot. Results: circZNF609 was highly expressed at PC tissues. circZNF609 declined cell colony ability, viability, migration and invasion and caused apoptosis. Furthermore, circZNF609 negatively regulated miR-186-5p, miR-186-5p inhibitor could reverse impacts of circZNF609. Finally, circZNF609 restrained the YAP1 and AMPK pathways by up-regulating miR-186-5p. Conclusion: Silencing circZNF609 restrained growth, migration and invasion of PC cells by upregulating miR-186-5p via YAP1 and AMPK pathways. HIGHLIGHTS 1. circZNF609 is highly expressed in PC tissues; 2. circZNF609 restrains cell growth, migration and invasion; 3. circZNF609 exerts its function by up-regulating miR-186-5p; 4. circZNF609 exerts its function by YAP1 and AMPK signaling pathways.

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“…It is upregulated in nasopharyngeal carcinoma, renal and breast cancer, and other cancers. Circ-ZNF609 knockdown dramatically inhibits cancer cell proliferation, invasion, and metastasis [188][189][190]. Bozzoni et al reported that circ-ZNF609 was strongly expressed in muscle cells, highly conserved evolutionarily, and contained a 753-nt ORF.…”

Section: Other Functional Peptidesmentioning

confidence: 99%

Emerging role of tumor-related functional peptides encoded by lncRNA and circRNA

et al. 2020

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Non-coding RNAs do not encode proteins and regulate various oncological processes. They are also important potential cancer diagnostic and prognostic biomarkers. Bioinformatics and translation omics have begun to elucidate the roles and modes of action of the functional peptides encoded by ncRNA. Here, recent advances in long non-coding RNA (lncRNA) and circular RNA (circRNA)-encoded small peptides are compiled and synthesized. We introduce both the computational and analytical methods used to forecast prospective ncRNAs encoding oncologically functional oligopeptides. We also present numerous specific lncRNA and circRNA-encoded proteins and their cancer-promoting or cancer-inhibiting molecular mechanisms. This information may expedite the discovery, development, and optimization of novel and efficacious cancer diagnostic, therapeutic, and prognostic protein-based tools derived from non-coding RNAs. The role of ncRNA-encoding functional peptides has promising application perspectives and potential challenges in cancer research. The aim of this review is to provide a theoretical basis and relevant references, which may promote the discovery of more functional peptides encoded by ncRNAs, and further develop novel anticancer therapeutic targets, as well as diagnostic and prognostic cancer markers.

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CircZNF609 promotes breast cancer cell growth, migration, and invasion by elevating p70S6K1 via sponging miR-145-5p

Cited by 69 publications

References 33 publications

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Anti-antioxidant impacts of circZNF609 silence in HaCaT cells through regulating miR-145

Silencing circular RNA circZNF609 restrains growth, migration and invasion by up-regulating microRNA-186-5p in prostate cancer

Emerging role of tumor-related functional peptides encoded by lncRNA and circRNA

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