Cis-Acting Elements Essential for Light Regulation of the Nuclear Gene Encoding the A Subunit of Chloroplast Glyceraldehyde 3-Phosphate Dehydrogenase in Arabidopsis thaliana

Park, Soo-Chul; Kwon, Hawk-Bin; Shih, Ming-Che

doi:10.1104/pp.112.4.1563

Cited by 33 publications

(19 citation statements)

References 25 publications

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“…Furthermore, they contain the AE-box (AGAAACAA), which mediates the light regulation of glyceraldehyde 3-P dehydrogenase (Park et al, 1996). These cis-acting elements are absent from the promoter region of OsPSY3, which explains the lack of response to light.…”

Section: The Different Induction Characteristics Of Rice Psys Correlamentioning

confidence: 99%

A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes

et al. 2008

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We here report on the characterization of a novel third phytoene synthase gene (PSY) in rice (Oryza sativa), OsPSY3, and on the differences among all three PSY genes with respect to the tissue-specific expression and regulation upon various environmental stimuli. The two already known PSYs are under phytochrome control and involved in carotenoid biosynthesis in photosynthetically active tissues and exhibit different expression patterns during chloroplast development. In contrast, OsPSY3 transcript levels are not affected by light and show almost no tissue-specific differences. Rather, OsPSY3 transcripts are up-regulated during increased abscisic acid (ABA) formation upon salt treatment and drought, especially in roots. The simultaneous induction of genes encoding 9-cis-epoxycarotenoid dioxygenases (NCEDs), involved in the initial steps of ABA biosynthesis, indicate that decreased xanthophyll levels are compensated by the induction of the third PSY gene. Furthermore, OsPSY3 and the OsNCEDs investigated were also induced by the application of ABA, indicating positive feedback regulation. The regulatory differences are mirrored by cis-acting elements in the corresponding promoter regions, with light-responsive elements for OsPSY1 and OsPSY2 and an ABA-response element as well as a coupling element for OsPSY3. The investigation of the gene structures and 5# untranslated regions revealed that OsPSY1 represents a descendant of an ancient PSY gene present in the common ancestor of monocots and dicots. Since the genomic structures of OsPSY2 and OsPSY3 are comparable, we conclude that they originated from the most recent common ancestor, OsPSY1.Carotenoids are lipophilic isoprenoids produced by all photosynthetic organisms as well as by some nonphotosynthetic bacteria and fungi. In animals, carotenoids come from the food chain and function as colorants and precursors for essential metabolites, such as retinal, retinol, and retinoic acid. In plants, carotenoids play their classical roles in light-harvesting complexes and photosynthetic reaction centers, where they absorb light and dissipate excess energy (for review, see Adams, 1992, 2000;Niyogi, 1999).The plant carotenoid biosynthetic pathway is localized in the plastid and has been molecularly elucidated in recent years (for review, see DellaPenna and Pogson, 2006). It diverges from C 3 carbon metabolism by the action of the enzyme deoxyxylulose phosphate synthase, followed by a series of enzymes of the socalled nonmevalonate pathway (for review, see Hunter, 2007), yielding isopentenyl diphosphate (IPP; C 5 ), the building block of all isoprenoids. IPP and its isomer dimethylallyl diphosphate (DMAPP) are then converted into carotenes through chain-elongating condensation reactions catalyzed by geranylgeranyl diphosphate synthase (GGPPS) and phytoene synthase (PSY; Fig. 1). The triene chromophore of phytoene (C 40 ), the first carotene formed, is then extended to form the colored undecaene in lycopene catalyzed by phytoene desaturase (PDS) and z-carotene desaturase (ZD...

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Section: The Different Induction Characteristics Of Rice Psys Correlamentioning

confidence: 99%

A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes

et al. 2008

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“…We found a total number of 19 putative LREs (Supplemental Fig. S3), including seven GATA boxes (Lam and Chua, 1989), six GT1 boxes (Villain et al, 1996), three I boxes (Giuliano et al, 1988), one AE box (Park et al, 1996), one ATCT motif (Conley et al, 1994), and one GGATAA element (Tepperman et al, 2001). GATA, GT1, and I boxes are cis-elements commonly existing in light-regulated promoters and shown to be essential for light-controlled transcriptional activity (Tobin and Kehoe, 1994;Terzaghi and Cashmore, 1995;Millar and Kay, 1996).…”

Section: Hyh Interacts With the Promoter Of Pex11bmentioning

confidence: 99%

“…The promoters of light-inducible genes in plants harbor various types of cis-elements, collectively named light-response elements (LREs), which are bound by transcription factors and mediate lightdependent gene activation (Ueda et al, 1989;Park et al, 1996;Lescot et al, 2002). To assess whether putative LREs are present in the promoter of PEX11b, we performed detailed in silico searches of the PlantCARE and the PLACE plant cis-element databases, using a 219-bp promoter region immediately upstream of the transcription start site (TSS) of this gene as query.…”

Section: Hyh Interacts With the Promoter Of Pex11bmentioning

confidence: 99%

Light Induces Peroxisome Proliferation in Arabidopsis Seedlings through the Photoreceptor Phytochrome A, the Transcription Factor HY5 HOMOLOG, and the Peroxisomal Protein PEROXIN11b

Desai

2008

Plant Physiology

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Peroxisomes are single membrane-delimited subcellular organelles that carry out numerous vital metabolic reactions in nearly all eukaryotes. Peroxisomes alter their morphology, abundance, and enzymatic constituents in response to environmental cues, yet little is known about the underlying mechanisms. In this work, we investigated the regulatory role of light in peroxisome proliferation in Arabidopsis (Arabidopsis thaliana). We provide evidence that light induces proliferation of peroxisomes in Arabidopsis seedlings and that the peroxisomal protein PEX11b plays an important role in mediating this process. The far-red light receptor phytochrome A (phyA) and the bZIP transcription factor HY5 HOMOLOG (HYH) are both required for the up-regulation of PEX11b in the light. We further demonstrate that the phyA and hyh mutants exhibit reduced peroxisome abundance, a phenotype that can be rescued by overexpressing PEX11b in these plants. The HYH protein is able to bind to the promoter of PEX11b, suggesting that the PEX11b gene is a direct target of HYH. We conclude that HYH and PEX11b constitute a novel branch of the phyA-mediated light signaling cascade, which promotes peroxisome proliferation during seedling photomorphogenesis.

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“…Further, the cis -regulatory motif HSE involved in heat shock response [46](fig. 1), a wounding-related WUN motif [46]and light-responsive elements AE box [47], GAG motif and LAMP element [38](data not shown) were predicted.…”

Section: Resultsmentioning

confidence: 99%

A Model System to Study the Environment-Dependent Expression of the <i>Bet v 1a</i> Gene Encoding the Major Birch Pollen Allergen

Tashpulatov¹,

Clement²,

Akimcheva³

et al. 2004

Int Arch Allergy Immunol

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Background: The major birch pollen allergen Bet v 1 (or Bet v 1a) is one of the main causes of seasonal type I allergies. Various environmental factors such as light, temperature and air pollution may influence the activity of the Bet v 1a gene. The creation of a model system to evaluate the role of environmental factors affecting the Bet v 1a gene expression would be highly desirable. We suggest the use of transgenic tobacco plants carrying a Bet v 1a promoter-reporter gene fusion as such a system. Methods: The promoter of the Bet v 1a gene was isolated with the use of the Universal Genome Walker kit (BD Biosciences Clontech, USA). Web Software was used to search for putative cis-regulatory elements within the promoter. Transgenic tobacco plants harboring the promoter-β-glucuronidase (GUS) reporter gene fusion were obtained via Agrobacterium tumefaciens-mediated transformation. Promoter activity was examined with histochemical and quantitative assays. Results: Structural analysis predicted elements responsible for pollen-specific, light-, stress- and hormone-mediated induction within the Bet v 1a promoter. The evaluation of GUS activity in transgenic tobacco plants showed that the Bet v 1a promoter is pollen-specific. Moreover, the Bet v 1a promoter is considered to be the strongest isolated pollen-specific promoter reported to date. It was shown that temperature and abscisic acid positively regulate the activity of the Bet v 1a promoter during pollen development, providing evidence for environment-dependent regulation of the Bet v 1a gene. Conclusions: A model system to study the effect of environmental factors on the expression of the Bet v 1a gene encoding the major birch allergen in pollen was generated. Additionally, we suggest that this system could be used to search for factors that inhibit the activity of the gene in pollen in order to reduce the potential allergenicity of birch trees.

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Cis-Acting Elements Essential for Light Regulation of the Nuclear Gene Encoding the A Subunit of Chloroplast Glyceraldehyde 3-Phosphate Dehydrogenase in Arabidopsis thaliana

Cited by 33 publications

References 25 publications

A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes

A Third Phytoene Synthase Is Devoted to Abiotic Stress-Induced Abscisic Acid Formation in Rice and Defines Functional Diversification of Phytoene Synthase Genes

Light Induces Peroxisome Proliferation in Arabidopsis Seedlings through the Photoreceptor Phytochrome A, the Transcription Factor HY5 HOMOLOG, and the Peroxisomal Protein PEROXIN11b

A Model System to Study the Environment-Dependent Expression of the <i>Bet v 1a</i> Gene Encoding the Major Birch Pollen Allergen

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