Cisplatin Induced Histological and Ultrastructural Alterations In Liver Tissue of Rat

Abstract

Background:Cisplatin is well-known anticancer drug often been used for treatment of various human malignancies. The hepatotoxic or nephrotoxic effect of drug is a result of altered histological and antioxidant status of the organ. In order to document the extent of cisplatin (CDDP) effect on liver we have studied long term treatment of cisplatin (CDDP) to rat. In our previous studies we have demonstrated changes in reduced glutathione (GSH) and glutathione related enzymes of liver followed by increased lipid peroxidation process. Materials: Light microscopy (LM) was carried out in liver tissue by haematoxylin and eosin staining. Electron microscopy was performed by staining with uranil acetate and lead citrate. Results: Recent reports depicts that CDDP treatment caused significant alteration at histopathological level showing increased vacuolation in hepatocytes. A noticeable change observed after drug treatment is large perilobular connective and expanded portal spaces. Morphological alterations after transmission electron microscopy (TEM) showed heterochromatic border in nucleus followed by prescence of large agglutinations of lysosomes, numerous rough endoplasmic reticulum (RER) and mitochondria has been observed as one of the significant effect of drug. Conclusion: Thus cytoarchitectural studies signify that long term of CDDP intervention at 0.4 mg/kg/day/animal caused least damage to liver.

The ameliorative influence of melatonin against hepatotoxicity-induced by taxol in adult rats

“…These activated Kupffer cells had deteriorated cytoplasm and malformed nuclei having electrondense chromatin materials, as well as extended filopodia. The present findings are alongside with previous investigations carried out by Kamble & Bhiwgade (2011) and Kandil et al (2021).…”

Histopathological and some biochemical effects of platinum drug on the liver and kidney of pregnant mice Mus musculus and their embryos

“…The prevailing data revealed several lesions in the maternal liver of both groups II and III including ; vacuolation, apoptosis, an increase of the eosinophil's of some hepatocytes in the portal area, congestion of the central vein, and dilatation of the sinusoids. The current findings were comparable to the findings of those (22)(23)(24)(25)(26)(27), how indicates that injecting rodents (rats) with the platinum-base anticancer at certain doses can induce programmed cell death, and some structural changes in the liver. The current findings were contrary to the findings of (25).…”

Light and Electron Microscopic Study on the Possible Protective Effect of Nigella Sativa Oil on Cisplatin Hepatotoxicity in Albino Rats

“…Some area of the cytoplasm showed rarefaction, lipid droplets and vacuoles. Other investigators found some of these changes [30][31][32] .…”