Citologia De Lavado Broncoalveolar De Cães: Comparação Entre Lâminas a Fresco E Conservadas Em Formol

Abstract

RESUMO:O lavado broncoalveolar em cães é um método diagnóstico recomendado em casos de enfermidades do trato respiratório inferior, quando exames de rotina não permitem um diagnóstico conclusivo. O exame baseia-se na análise citológica e confecção de lâminas com o material a fresco, ou seja, logo após a coleta, o que pode inviabilizar a técnica em casos de difícil acesso aos laboratórios especializados. Para isso, faz-se necessário um meio de conservação das amostras, aumentando o tempo de vida útil do material a ser analisado. Assim, realizou-se o lavado broncoalveolar em quatorze cães adultos saudáveis. As amostras foram separadas em duas alíquotas: a primeira confeccionada a fresco por sedimentação em câmara de Suta, e a segunda processada uma semana depois, com a amostra conservada em formol. As lâminas foram coradas pelo corante rápido Panótico. Avaliou-se o volume infundido e o volume recuperado, o aspecto macroscópico, a contagem de células nucleadas, a análise diferencial de células e a análise descritiva das lâminas quanto à celularidade, presença de muco, hemácias, leucócitos e células, íntegras ou degeneradas. A análise estatística foi realizada com teste t, para amostras pareadas com p<0,05. Foram encontrados aumento significativo na quantidade de linfócitos e diminuição do número de macrófagos nas amostras conservadas em formol. As demais observações foram similares em ambos os grupos. Portanto, a conservação do material do lavado broncoalveolar de cão em formol, durante uma semana, preservou as células, tornando viável a técnica do lavado broncoalveolar. Palavras-chave: citologia; cão; formol; lavado broncoalveolar CYTOLOGY OF BRONCHOALVEOLAR LAVAGE OF DOGS: COMPARISON AMONG LAMINA TO FRESH AND CONSERVED IN FORMOL ABSTRACT:The bronchoalveolar lavage in dogs is a diagnostic method recommended in cases of illnesses of the caudal respiratory tract, when routine exams do not allow a diagnosis. The exam relies on the cytological analysis and fresh smear immediately after sampling, which may difficult the adequate processing at the specialized laboratories. Accordingly, a sample conservation media which increases the material time span to be analyzed would be desirable. In the present study, bronchoalveolar lavage was performed in fourteen healthy adult dogs. Samples were separate in two aliquots: one freshly made by in chamber of sedimentation, and another processed one week later, with the sample preserved in formalin. Slides were stained by quick Romanovsky-type stain (Panótico Rápido ® , Laborclin ® , Pinhais, Brazil). The infused volume, the recovered volume, the macroscopic aspect, the counting of nucleated cells, the differential cell analysis and the smear descriptive analysis such as cellularity, mucus presence, erythrocytes, leukocytes and degenerate cells were evaluated. The statistical analysis was performed with T test, for paired samples with p<0.05. Significant increase was found in the amount of lymphocytes and decrease of the macrophage numbers in formalin preserved samples. All other ob...

Fungal pneumonia in dogs and cats with pulmonary clinical signs in southern Brazil

“…Then, a sterile siliconized catheter for enteral feeding (adapted by removing the metallic end) adjusted appropriately for the size of the animal was inserted into the tracheal tube, until resistance encountered, next was infused 0.9% saline solution (up to 5ml/kg for dogs and up to 3ml/kg for cats), followed by 5ml of air to prevent the solution remaining in the catheter. In animals with a body weight over 10 kg, 20ml of physiological solution was standardized (Ribas et al 2010). The infused solution was immediately recovered using a sterile syringe.…”

Cytological analysis of bronchoalveolar lavage fluid of dogs with Visceral Leishmaniasis and infused with bone marrow mononuclear cells

“…In our study this increase can be strongly associated with higher inflow of similar cells to lymphocytes to the lungs due to the infusion of BMMC and these BMMC can be either hematopoietic origin and mesenchymal. In contrast, the macrophage differential counts in dogs naturally infected pre and post infusion of BMMC (Table 03) observed to be similar to values found in healthy dogs (BARÇANTE, 2008), even if the resident macrophages are the predominant cell population found in BALF (HAWKINS, 1990;ANDREASEN, 2003;RIBAS, 2010;FINKE, 2013) It is known that the BMMC can also increase the number of macrophages, from the origin of circulating monocytes can differentiate into macrophages (HUSSELL and BELL, 2014), which would explain the numerical increase of over 40% in the differential count in two animals (2/4) of TPG.…”

“…In general, the ratio of the obtained volume was low, considering the volume of injected saline solution, the average yield was 15% of the injected volume, this volume close to that obtained by Mello (2002) Woods et al (2013) and Woods et al (2014), but lower than the amount aspirated by Ribas (2010).…”

“…The liquid obtained was presented as a colorless, slightly cloudy, with particulate matter and presence of surfactant, appropriate macroscopic characteristics for the collected material (LESSA, 2005;RIBAS, 2010). The presence of surfactant in the fluid is verified by the presence of foam in the aspirate collection tube, showing that the obtained fluid was withdrawn from LRT (HAWKINS; DeNICOLA; KUEHN, 1990;FINKE 2013).…”

“…Obtaining fluid allows a cytological and microbiological exploration (BASSO, 2008;RIBAS, 2010). The main cells found in abundance in sputum and bronchoalveolar lavage fluid (BALF) in acute processes are neutrophils.…”