Citral-induced analgesia is associated with increased spinal serotonin, reduced spinal nociceptive signaling, and reduced systemic oxidative stress in arthritis

Mota, Clarissa M.D.; Rodrigues-Santos, Caroline; Carolino, Ruither Oliveira Gomes; Anselmo-Franci, Janete Aparecida; Branco, Luiz Guilherme de Siqueira

doi:10.1016/j.jep.2019.112486

Cited by 19 publications

(13 citation statements)

References 66 publications

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“…As directed by the manufacturer, the concentrations of IL‐1β, IL‐6, and TNF‐α in rat blood were evaluated using enzyme‐linked immunosorbent assay (ELISA) kits. From the respective standard curves, the concentrations of IL‐1β, IL‐6, and TNF‐α were calculated …”

Section: Methodsmentioning

confidence: 99%

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Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Cui

Sreeharsha

et al. 2020

IUBMB Life

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Background Rheumatoid arthritis (RA) is associated with joint damage. Effectiveness of embelin has been established in a wide variety of inflammatory disorders, but its utility as a therapeutic agent is limited by its poor absorption, rapid metabolism, and fast systemic elimination. To apprehend these limitations, we propose to use highly bioavailable embelin‐loaded chitosan nanoparticles (CS‐embelin NPs) for the treatment of RA. Methods The rats were made arthritic using a subcutaneous injection with 0.1 ml complete Freund's adjuvant (CFA) into the footpad of the left hind paw. CS‐embelin NPs (25 and 50 mg/kg) was administered from day 15 to day 28 after adjuvant injection. After the experimental period, the animals were sacrificed and various biochemical markers were assessed. Results Arthritic score and paw swelling were significantly reduced after treatment with CS‐embelin NPs. Arthritis‐induced rats showed a significant increase in malondialdehyde (MDA) and nitric oxide (NO) with a concomitant reduction of antioxidants in the paw tissue. CS‐embelin NPs (25 and 50 mg/kg) reduced MDA and NO levels and restored antioxidant levels to normalcy by mitigating oxidative stress. The arthritic rats exhibited elevated tumor necrosis factor‐α (TNF‐α), interleukin‐6 (IL‐6) and interleukin‐1beta (IL‐1β) serum concentrations, upregulated TNF‐ α and IL–6 protein levels and upregulated nuclear factor‐kB (NF‐kB) mRNA expression in paw tissues. Treatment with CS‐embelin NPs (25 and 50 mg/kg) significantly reduced serum levels and down‐regulated inflammatory markers to normalcy, dose‐dependently. Conclusion The results suggest that CS‐embelin NPs displayed a protective effect against adjuvant‐induced arthritis in rats mediated through antioxidant and anti‐inflammatory effects.

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Section: Methodsmentioning

confidence: 99%

“…From the respective standard curves, the concentrations of IL-1β, IL-6, and TNF-α were calculated. 31…”

Section: Measurement Of Proinflammatory Cytokines Levelsmentioning

confidence: 99%

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Cui

Sreeharsha

et al. 2020

IUBMB Life

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“…For example, M. spicata L. EO and its main constituents, such as menthol, carvone, and limonene, demonstrated analgesic effects in animal models, while spearmint oil was able to reduce pain in osteoarthritis patients [ 92 ]. Recently, Mota and colleagues reported the analgesic effect of citral [ 93 ], a monoterpene aldehyde identified in the EOs of several plants, such as lemongrass ( Cymbopogon citratus (DC.) Stapf.)…”

Section: Essential Oils and Their Chemical Components In The Treatmentioning

confidence: 99%

“…[ 94 ] and ginger ( Zingiber officinale Roscoe) [ 95 , 96 ]. It was demonstrated that a single administration of citral (at concentrations of 100 and 300 mg/kg) reversed FCA-induced mechanical allodynia in arthritic rats [ 93 ].…”

Section: Essential Oils and Their Chemical Components In The Treatmentioning

confidence: 99%

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Marrelli

Amodeo

Perri

et al. 2020

Plants

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Essential oils (EOs) are known to possess a number of beneficial properties. Their antimicrobial, anti-inflammatory, antioxidant, antidiabetic, and cancer-preventing activities have been extensively reported. Due to their wide use as food preservers and additives, as well as their use in agriculture, perfumes, and make-up products, these complex mixtures of volatile compounds have gained importance from a commercial point of view, not only in the pharmaceutical industry, but also in agronomic, food, cosmetic, and perfume industries. An analysis of the recent scientific literature allowed us to highlight the presence of an increasing number of studies on the potential antiarthritic properties of EOs and their main constituents, which seems to suggest a new interesting potential therapeutic application. The aim of this review is to examine the current knowledge on the beneficial effects of essential oils in the treatment of arthritic diseases, providing an overview of the reports on the in vivo and in vitro effects of EOs. Furthermore, this review critically examines the recent findings on the potential roles of the main components of EOs in the exerted beneficial effects. Obtained negative results are also reported.

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“…Likewise, the activation of the TLR4, TLR2/Dectin-1, and CB 2 receptor/ATP-Sensitive K + channel pathways by citral were involved in its anti-inflammatory effects (Gonçalves et al 2020). There is evidence D r a f t about the participation of spinal serotonin in the antinociception of citral in an arthritic rat model (Mota et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Citral inhibits the nociception in the rat formalin test: effect of metformin and blockers of opioid receptor and the NO-cGMP-K⁺ channel pathway

Ortiz

Cariño-Cortés

Pérez

et al. 2022

Can. J. Physiol. Pharmacol.

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The objective of the present study was to scrutinize the effect of nitric oxide (NO), cGMP, potassium channel blockers and metformin on the citral-produced peripheral antinociception. The rat paw 1% formalin test was used to assess nociception and antinociception. Rats were treated with local peripheral administration of citral (10-100 µg/paw). The antinociception of citral (100 µg/paw) was evaluated with and without the local pretreatment of naloxone, NG-L-nitro-arginine methyl ester (L-NAME, a NO synthesis inhibitor), 1H-(1,2,4)-oxadiazolo(4,2-a)quinoxalin-1-one (ODQ, a soluble guanylyl cyclase inhibitor), metformin, opioid receptors antagonists, and K+ channel blockers. Injection of citral in the rat paw significantly decreased the nociceptive effect of formalin administration during the two phases of the test. Local pretreatment of the paws with L-NAME and ODQ did not reduced the citral-induced antinociception. Glipizide or glibenclamide (Kir6.1-2; ATP-sensitive K+ channel blockers), tetraethylammonium or 4-aminopyridine (KV; voltage-gated K+ channel blockers) or charybdotoxin (KCa1.1; big conductance calcium-activated K+ channel blocker) or apamin (KCa2.1-3; small conductance Ca2+-activated K+ channel antagonist), or metformin, but not the opioid antagonists, reduced the antinociception of citral. Citral produced peripheral antinociception during both phases of the formalin test. These effects were due to the activation of K+ channels and a biguanide-dependent mechanism.

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Citral-induced analgesia is associated with increased spinal serotonin, reduced spinal nociceptive signaling, and reduced systemic oxidative stress in arthritis

Cited by 19 publications

References 66 publications

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Citral inhibits the nociception in the rat formalin test: effect of metformin and blockers of opioid receptor and the NO-cGMP-K⁺ channel pathway

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Citral-induced analgesia is associated with increased spinal serotonin, reduced spinal nociceptive signaling, and reduced systemic oxidative stress in arthritis

Cited by 19 publications

References 66 publications

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Antiarthritic effect of chitosan nanoparticle loaded with embelin against adjuvant‐induced arthritis in Wistar rats

Essential Oils and Bioactive Components against Arthritis: A Novel Perspective on Their Therapeutic Potential

Citral inhibits the nociception in the rat formalin test: effect of metformin and blockers of opioid receptor and the NO-cGMP-K+ channel pathway

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Product

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Citral inhibits the nociception in the rat formalin test: effect of metformin and blockers of opioid receptor and the NO-cGMP-K⁺ channel pathway