Citrate Stabilized Silver Nanoparticles

Bhattarai, Nisha; Khanal, Subarna; Pudasaini, Pushpa Raj; Pahl, Shanna; Romero-Urbina, Dulce

doi:10.4018/ijnmc.2011070102

Cited by 11 publications

(9 citation statements)

References 39 publications

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“…Battharai et al was able to show the presence of Ag 0 by performing a TEM investigation. Additionally, the existence of Ag + adsorbed on Ag 0 was discovered, and it was established that as Ag ions coalesce, as they formed AgNPs carrying an extra positive charge, which was that of Ag + [ 42 ]. Similarly, this occurs in AgNPs that contain mixtures of Ag 0 and Ag + ; as Ag + coalesces into larger Ag particles, these new particles become positively charged.…”

Section: Introductionmentioning

confidence: 99%

“…The strong ionic presence in the solution after filtration was responsible for the peak shift. Furthermore, the presence of three species in an AgNP solution is important, as we propose a new mechanism for how each of these three species interacts with the bacterial cell [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

“…The establishment of microbial resistance to silver via bacterial plasmids has been studied by geneticists at a molecular level [ 46 ]. The continuous leaching of silver ions by AgNPs [ 42 ] creates a favorable antimicrobial environment.…”

Section: Introductionmentioning

confidence: 99%

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Ultrastructural changes in methicillin-resistantStaphylococcus aureusinduced by positively charged silver nanoparticles

Romero-Urbina¹,

Lara²,

Velázquez‐Salazar³

et al. 2015

Beilstein J. Nanotechnol.

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SummarySilver nanoparticles offer a possible means of fighting antibacterial resistance. Most of their antibacterial properties are attributed to their silver ions. In the present work, we study the actions of positively charged silver nanoparticles against both methicillin-sensitive Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. We use aberration-corrected transmission electron microscopy to examine the bactericidal effects of silver nanoparticles and the ultrastructural changes in bacteria that are induced by silver nanoparticles. The study revealed that our 1 nm average size silver nanoparticles induced thinning and permeabilization of the cell wall, destabilization of the peptidoglycan layer, and subsequent leakage of intracellular content, causing bacterial cell lysis. We hypothesize that positively charged silver nanoparticles bind to the negatively charged polyanionic backbones of teichoic acids and the related cell wall glycopolymers of bacteria as a first target, consequently stressing the structure and permeability of the cell wall. This hypothesis provides a major mechanism to explain the antibacterial effects of silver nanoparticles on Staphylococcus aureus. Future research should focus on defining the related molecular mechanisms and their importance to the antimicrobial activity of silver nanoparticles.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ultrastructural changes in methicillin-resistantStaphylococcus aureusinduced by positively charged silver nanoparticles

Romero-Urbina¹,

Lara²,

Velázquez‐Salazar³

et al. 2015

Beilstein J. Nanotechnol.

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“…Other studies have shown that AgNP surfaces adsorb Ag+ ions in silver colloidal solutions. Our final solution contained three species of silver: Ag0 (AgNPs), Ag+ (silver ions), and Ag0/Ag+ (Ag+ adsorbed on Ag0) [ 2 , 95 ]. The presence of AgNPs was documented by transmission electron microscopy (TEM) imaging [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans

Pimentel-Acosta

Ramírez-Salcedo

Morales-Serna

et al. 2020

IJMS

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The mechanisms of action of silver nanoparticles (AgNPs) in monogenean parasites of the genus Cichlidogyrus were investigated through a microarray hybridization approach using genomic information from the nematode Caenorhabditis elegans. The effects of two concentrations of AgNPs were explored, low (6 µg/L Ag) and high (36 µg/L Ag). Microarray analysis revealed that both concentrations of AgNPs activated similar biological processes, although by different mechanisms. Expression profiles included genes involved in detoxification, neurotoxicity, modulation of cell signaling, reproduction, embryonic development, and tegument organization as the main biological processes dysregulated by AgNPs. Two important processes (DNA damage and cell death) were mostly activated in parasites exposed to the lower concentration of AgNPs. To our knowledge, this is the first study providing information on the sub-cellular and molecular effects of exposure to AgNPs in metazoan parasites of fish.

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“…In addition, the functionalization of metal nanoparticles with biomolecules is a limitation due to the absence of capping [30]. Similar reports on silver nanoparticle synthesis while using sodium borohydride have shown that it causes instability in nanoparticles and leads to aggregation via coalescence phenomena [31]. Gold nanoparticles that are synthesized only with NaBH 4 require controlled synthetic conditions like stirring in the dark for one hour and further storage at 4 • C for stable nanoparticles [32].…”

Section: Uv-vis-nir Measurementsmentioning

confidence: 99%

A Novel Approach towards Synthesis and Characterization of Non-Cytotoxic Gold Nanoparticles Using Taurine as Capping Agent

et al. 2019

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Metal gold nanoparticles are of great interest due to their unique physico-chemical properties and their potential to be used as nano-probes in biosensors, drug delivery, and therapeutic applications. Currently, many capping agents are used for metal gold nanoparticles, such as cetyltrimethylammonium bromide (CTAB) and tri-sodium citrate that have been reported to be toxic and hinders biological applications. To address this issue, we report, for the first time, the use of taurine as a stable non-cytotoxic capping agent for synthesizing gold nanoparticles by using an in situ wet-chemical method. This facile method resulted in monodisperse gold nanospheres with a high yield and stability. Monodisperse gold nanospheres with average diameters of 6.9 nm and 46 nm were synthesized at a high yield with controlled morphology. Temperature played a critical role in determining the size of the taurine-capped gold nanoparticles. The subtle changes in the reaction parameters had a tremendous effect on the final size of nanoparticles and their stability. The synthesized nanoparticles were characterized by using optical spectroscopy, a ZetaSizer, a NanoSight, Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD), X-ray Photon Spectroscopy (XPS) and Electron Microscopy to understand their physico-chemical properties. Taurine was explored as a capping and stabilizing agent for gold nanospheres, which were evaluated for their toxicity responses towards human liver carcinoma cells (HepG2) via MTT assay.

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Citrate Stabilized Silver Nanoparticles

Cited by 11 publications

References 39 publications

Ultrastructural changes in methicillin-resistantStaphylococcus aureusinduced by positively charged silver nanoparticles

Ultrastructural changes in methicillin-resistantStaphylococcus aureusinduced by positively charged silver nanoparticles

Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons from Caenorhabditis elegans

A Novel Approach towards Synthesis and Characterization of Non-Cytotoxic Gold Nanoparticles Using Taurine as Capping Agent

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