Citric acid production from partly deproteinized whey under non-sterile culture conditions using immobilized cells of lactose—positive and cold-adapted Yarrowia lipolytica B9

Arslan, Nazlı Pınar; Aydoğan, Mehmet; Taşkın, Mesut

doi:10.1016/j.jbiotec.2016.05.033

Cited by 39 publications

(27 citation statements)

References 39 publications

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“…Conversely, there was a continuous increase in FB even at the highest (NH4)2SO4 of 4 g/L. This finding is in good agreement with the fact that excessive nitrogen concentration increases fungal growth but decreases the amount of CA produced (Hang et al 1977, Vandenberghe et al 1999, Soccol et al 2006, Auta et al 2014, Arslan et al 2016.…”

Section: Discussionsupporting

confidence: 74%

USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4

Arslan

Aydoğan

2020

Trakya University Journal of Natural Sciences

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Mulberry pomace (MP) is a waste material obtained after the production of pekmez, a traditional Turkish food. This study was performed to test the usability of MP as substrate for citric acid (CA) production by Aspergillus niger MT-4 for the first time. In the study, some culture conditions were also optimized to increase CA production in MP-based medium. Moisture, total carbohydrate, water-soluble carbohydrate, protein, lipid and ash contents of MP were determined as 31.1, 47.1, 1.3, 13.4, 1.8 and 1.4%, respectively. Experiments were carried out in 250 mL flasks containing 100 mL of production medium. Optimal MP concentration for both fungal biomass (FB) and CA production was determined as 120 g/L. All concentrations of KH2PO4 added to MP-based medium were found to decrease CA production but increase FB production. Optimal concentrations of MgSO4 and (NH4)2SO4 for CA production were found as 1 and 2 g/L, respectively. The other optimal parameters were determined as an initial pH of 7.0 and an incubation period of 5 days. Under the optimized culture conditions, the amount of CA produced was determined as 24.6 g/L. On day 5, Yp/s, Yp/x and Yx/s were calculated as 0.2 g CA/g MP, 1.43 g CA/g FB and 0.14 g FB/g MP, respectively. Özet: Dut posası (DP), geleneksel bir Türk yiyeceği olan pekmez'in üretiminden sonra ortaya çıkan bir atık maddedir. Bu çalışma, Aspergillus niger MT-4 ile sitrik asit (SA) üretimi için DP'nin substrat olarak kullanılabilirliğini test etmek için gerçekleştirilmiştir. Çalışmada ayrıca, SA üretimini artırmak için bazı kültür koşulları da optimize edilmiştir. MP'nin nem, toplam karbonhidrat, suda-çözünür karbonhidrat, protein, lipid ve kül içerikleri sırasıyla %31,1; 47,1; 1,3; 13,4; 1,8 ve 1,4 olarak belirlenmiştir. Deneyler, üretim besiyerinin 100 ml'sini içeren 250 ml'lik erlenler içerisinde gerçekleştirilmiştir. Hem fungal biyokütle (FB) hem de SA üretimi için optimum DP konsantrasyonu, 120 gr/lt olarak belirlenmiştir. DP bazlı ortama eklenen tüm KH2PO4 konsantrasyonlarının SA üretimini azalttığı fakat FB üretimini artırdığı belirlenmiştir. SA üretimi için MgSO4 ve (NH4)2SO4'ün optimal konsantrasyonları sırasıyla 1 ve 2 gr/lt olarak bulunmuştur. Diğer optimal parametreler, başlangıç pH'sı 7,0 ve inkübasyon süresi 5 gün olarak belirlenmiştir. Optimize edilmiş kültür koşulları altında, üretilen SA miktarı 24,6 gr/lt olarak belirlenmiştir. Beşinci günde, Yp/s; Yp/x ve Yx/s sırasıyla 0,2 gr SA/gr DP; 1,43 gr SA/gr FB ve 0,14 gr FB/gr DP olarak hesaplanmıştır. DP'nin SA dahil mikrobiyal metabolitlerin üretimi için fermentasyon substratı olarak kullanılabilirliği ilk kez bu çalışmada test edilmiştir.

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Section: Discussionsupporting

confidence: 74%

USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4

Arslan

Aydoğan

2020

Trakya University Journal of Natural Sciences

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“…lipolytica . Namely, high C/N molar ratio is a key parameter for CA production by yeast (Arslan, Aydogan, & Taskin, ; Levinson, Kurtzman, & Kuo, ; Ochoa‐Estopier & Guillouet, ; Papanikolaou, Muniglia, Chevalot, Aggelis, & Marc, ). Depending on C/N molar ratio, biomass formation (i.e., cell growth), CA production, or lipid accumulation could be favoured, demonstrating thus the importance of this parameter for CA synthesis (Beopoulos et al, ; Madzak, ).…”

Section: Introductionmentioning

confidence: 99%

Screening various Yarrowia lipolytica strains for citric acid production

Çarşanba

Papanikolaou

Fickers

et al. 2019

Yeast

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Citric acid (CA) productivity by Yarrowia lipolytica dependents on strain type, carbon source, carbon to nitrogen (C/N) molar ratio as well as physicochemical conditions (pH, temperature, oxygen transfer rate, etc.). In the current study, 10 different Y. lipolytica strains were first challenged in shake-flask culture for CA production in a glucose-based media under nitrogen-limited conditions. For the most promising one, strain K57, CA productivity was monitored during culture in batch bioreactor for three initial C/N molar ratio (167, 367, and 567 Cmol/Nmol). The highest CA yield (0.77 g/g glucose), titre (72.3 g/L CA), and productivity (0.04 g/g.h) were found for C/N ratio of 367. However, the highest growth rate was obtained for an initial C/N ratio of 167. From these results, Y. lipolytica strain K57 could be considered to produce CA at higher titre on glucose-based medium in batch bioreactor than others Y. lipolytica strain reported in the literature.

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“…With alginate immobilized cold adapted and lactose positive Y. lipolytica strain B9, CA was produced from partly deproteinized cheese whey after temperature treatment for 15 min at 90 • C. In optimal operational conditions (20 • C, pH 5.5, lactose content 20 g/L), CA was produced with titer of 33 g/L after 120 h of culture. In those conditions, the CA/iCA ratio was equal to 6.8 [47]. Non-aseptic OMW enriched with glycerol (50 g/L) was also used as substrates for CA production by the Y. lipolytica strain LGAM s(7) in the bioreactor (5 L working volume) at 25 • C and pH controlled at 2.8.…”

Section: Citric Acidmentioning

confidence: 99%

“…In contrast, CA production was maximal at neutral pH [21]. Carbon sources considered for iCA synthesis were n-alkane [58], rapeseed oil [59], ethanol [19], sunflower oil [60], frying oil waste [61], straw hydrolysate [61], milk whey [47] or raw glycerol [18,42].…”

Section: Iso-citric Acidmentioning

confidence: 99%

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

2020

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Sugar alcohols and organic acids that derive from the metabolism of certain microorganisms have a panoply of applications in agro-food, chemical and pharmaceutical industries. The main challenge in their production is to reach a productivity threshold that allow the process to be profitable. This relies on the construction of efficient cell factories by metabolic engineering and on the development of low-cost production processes by using industrial wastes or cheap and widely available raw materials as feedstock. The non-conventional yeast Yarrowia lipolytica has emerged recently as a potential producer of such metabolites owing its low nutritive requirements, its ability to grow at high cell densities in a bioreactor and ease of genome edition. This review will focus on current knowledge on the synthesis of the most important sugar alcohols and organic acids in Y. lipolytica.

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Citric acid production from partly deproteinized whey under non-sterile culture conditions using immobilized cells of lactose—positive and cold-adapted Yarrowia lipolytica B9

Cited by 39 publications

References 39 publications

USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4

USE OF MULBERRY POMACE AS SUBSTRATE FOR CITRIC ACID PRODUCTION BY Aspergillus niger MT-4

Screening various Yarrowia lipolytica strains for citric acid production

Sugar Alcohols and Organic Acids Synthesis in Yarrowia lipolytica: Where Are We?

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