Citric acid production in liquid waste from a leaf-protein production plant: Effects of sugar and potassium ferrocyanide

Chanda, Sukalpa; Chakrabarti, Snigdha; Matai, S.

doi:10.1016/0269-7483(90)90145-i

Cited by 8 publications

(5 citation statements)

References 6 publications

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“…The maximum amount of the citric acid and yield (46.71 ±0.01g/L, 31.14±0.01) were exhibited at 0.15% K4Fe(CN)6. It may be due to the fact that potassium ferrocyanide promotes the activity of some citric acid condensing enzyme by inhibiting the poisonous effect of some ions such as iron, zinc, copper, magnesium, calcium, potassium, and sodium [38]. The obtained results were in line with a previous study [39].…”

Section: Effect Of Potassium Ferrocyanide Addition To Molasses Medium...supporting

confidence: 90%

Improvement of Citric Acid Production by Gamma Radiated Aspergillus niger Using Cane Molasses

Khattab

Emam

Swelim

et al. 2022

Arab Journal of Nuclear Sciences and Applications

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Citric acid is in greater demand globally as a result of both population growth and industrialization. Aspergillus niger is a workhorse for the production of citric acid. Cane molasses have been chosen as a novel and economically viable substrate as a result of the rising demand for citric acid and the need for substitute materials as substrates in recent years. Therefore, the present study was conducted to improve citric acid production from A. niger using by-product of sugar (sugarcane molasses) through gamma radiation. Aspergillus niger was isolated from soil and confirmed by molecular identification based on beta tubulin gene sequencing which compared with available beta tubulin gene sequencing in the NCBI database. The NCBI database showed the highest percentage of similarity being 100 with A. niger. The fermentation process was performed in shaking flasks using sugarcane molasses. Data revealed that significantly highest production of citric acid (31.30 ±0.17 g/L) was obtained in a medium containing (15%) substrate level. In addition, the maximum productivity of citric acid was achieved at pH 6. Spore suspensions of A. niger were subjected to different doses of gamma radiation. Results clarified that the maximum amount (42.82 ±0.62 g/L) of citric acid was observed at 1.0 KGy. The amount of acid produced was increased by 1.11 fold higher than the control by the addition of 3% (v/v) ethanol after 24 hour of fermentation time. Moreover, the addition of 0.15% K4Fe (CN)6 enhanced the citric acid manufacturing. Furthermore, citric acid productivity from immobilized gamma irradiated A. niger spores was increased by 1.2 times more than that of the free ones. Citric acid was separated from the fermentation broth by precipitation method using CaCl2. In addition, FT-IR Analysis of the extracted citric acid from A. niger confirmed that there is no change in the main characteristic absorption bands of the standard citric acid.

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Section: Effect Of Potassium Ferrocyanide Addition To Molasses Medium...supporting

confidence: 90%

Improvement of Citric Acid Production by Gamma Radiated Aspergillus niger Using Cane Molasses

Khattab

Emam

Swelim

et al. 2022

Arab Journal of Nuclear Sciences and Applications

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“…Also, C/N ratio of Cabbage, Alfalfa and Spinach were equal to about 20:1,while the other 3 crops contain nearly the same ratio, 10:1 (Table 1). Similar results was obtained for Sugar beet with the author Chanda et al (1990b), while with respect to Turnip, the present result was nearly double than that obtained by Chanda et al (1990b). Several workers reported that the C/N ratio (20:1 or 10:1) was suitable for growing different microorganisms (yeast and fungi) to yield useful metabolites Rashad et al(1990); Chanda et al (1990b) and Jwanny et al 1989 and.…”

Section: Amino Acid Composition Of Leaf Protein Concentratessupporting

confidence: 90%

“…Similar results was obtained for Sugar beet with the author Chanda et al (1990b), while with respect to Turnip, the present result was nearly double than that obtained by Chanda et al (1990b). Several workers reported that the C/N ratio (20:1 or 10:1) was suitable for growing different microorganisms (yeast and fungi) to yield useful metabolites Rashad et al(1990); Chanda et al (1990b) and Jwanny et al 1989 and. Chanda et al 1990a andSim andHanger,1995 fortified beet whey, turnip whey and saurkraut brine (a by-product of cabbage processing industry) used with sucrose or molasses to give 10-15 % total sugar in the cultivation medium to produce protein,citric acid or other useful metabolites.…”

Section: Amino Acid Composition Of Leaf Protein Concentratessupporting

confidence: 90%

Chemical Constituents and Nutritive Value of 6 Local Vegetable Leaves by-Products

Rashad

Moharib²,

Abdou³

2000

Journal of Soil Sciences and Agricultural Engineering

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Leaf protein concentrates (LPC) of cabbage (Brassica oleracea),sugar beet (Beta vulgaris), alfalfa (Medicago sativa), turnip(Brassica rapa),spinach(Spinacia oleracea) and artichoke(Cynara scolymus) were extracted and determined. Amino acid composition of the obtained LPC , s was balanced and exceeded values recommended by FAO to serve as a high-quality food. Essential amino acid levels in the 6 leaf proteins obtained were compared favourably with human (FAO ref.Patterns) and chick requirements, the results indicating that all LPC , s contained well above the required amounts of preschool child and for chicks,except the amount of methionine,lysine and isoleucine.The deproteinized juice (whey) which pose serious disposal problems were tested as possible substrates for utilization of different microorganisms. All the tested whey contained reasonable amounts of sugar and nitrogen which could be used as such as a media. Six yeast strains and two edible fungi can grow well on the 6 tested wheys except Candida maltosa and Lentinus edodes failed to grow on Cynara scolymus. and Brassica oleracea whey could be considered as the most potential substrate for growing the 8 organisms used in this study,The LPC produced in the present study have suitable amounts of required essential amino acids good nutritional values, when incorporated into animal foods and human feeds. Also the wheys were used as substrates for growing microorganisms and production of protein and other useful metabolites having nutritional and biological values.

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“…The levels of Fe, Mn, Zn, Cu, and Mg in the medium 11 M-H were 1.68, 0.22, 1.17, 0.52, and 17.75 mg l -1 , respectively. Except for Mg, these values are much lower than those reported by Chanda et al 15 and Sanchez-Marroquin et al 28 for canesugar molasses.…”

Section: Selection Of the Ranges Of The Variablescontrasting

confidence: 58%

“…12 The yields of citric acid are drastically reduced especially in the presence of metal ions and minerals 13 and hence, these are binded and inactivated or precipitated by pretreatment of the substrate with potassium hexacyanoferrate II. [14][15] Another important factor is the inclusion of 1-3% methanol or other low molecular weight alcohols which stimulates citric acid production by affecting growth and sporulation through the action on the cell permeability, spatial organization of the membrane, or changes in lipid composition of the cell wall. 16 A relationship between alcohol and tolerance to Mn, Fe, and Zn has also been suggested.…”

Section: Introductionmentioning

confidence: 99%

Interactions between pretreatment and nutrient concentrations of mussel processing effluents for citric acid production

Pintado

González

Murado

1997

Enzyme and Microbial Technology

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Pretreatment of mussel processing effluents for citric acid production by Aspergillus niger (CBS 262-65) in submerged culture was studied by means of an orthogonal full factorial experimental design with four independent variables: initial levels of hexacyanoferrate II, methanol, phosphate, and carbon source. The empirical model thus obtained showed the ineffectiveness of the hexacyanoferrate treatment (contrarily that occurs with molasses) and at the same time, a series of complex interactions affecting the acid production not described in previous studies concerned by the separate effects of the variables here considered. Basically, the main effect of the hexacyanoferrate is toxic while the presence of methanol seems to involve morphological changes that increase the superficial area of the mycelial pellets, thereby promoting an unspecific metabolic activation. It therefore enhances the toxic effect of hexacyanoferrate, but also favors citric acid accumulation. The net response depends on the concentrations of the carbon source and phosphorus.

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Citric acid production in liquid waste from a leaf-protein production plant: Effects of sugar and potassium ferrocyanide

Cited by 8 publications

References 6 publications

Improvement of Citric Acid Production by Gamma Radiated Aspergillus niger Using Cane Molasses

Improvement of Citric Acid Production by Gamma Radiated Aspergillus niger Using Cane Molasses

Chemical Constituents and Nutritive Value of 6 Local Vegetable Leaves by-Products

Interactions between pretreatment and nutrient concentrations of mussel processing effluents for citric acid production

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