Citric acid production with alginate bead entrappedAspergillus niger ATCC 9142

Abstract

Aspergillus niger ATCC 9142 mycelium was entrapped in calcium alginate beads and employed in an air-lift completely stirred reactor for continuous production of citric acid. Maximum yield obtained from 10% (w/v) sucrose was 12 g dm(-3) with about 40% fermentation efficiency. Maximum rate of production 70 mg g(-1) h(-1) was about five times that obtained in classical batch fermentation.

Influence of alcohols on citric acid production by Aspergillus niger A-9 entrapped in polyacrylamide gels

“…glass carriers (Heinrich & Rehm, 1982), entrapment in calcium alginate (Cruz, Cruz, Belini, Belote, & Vieira, 1998;Vaija, Linko, & Linko, 1982), polyurethane foams (Lee, Lee, & Chang, 1989), agar (Borglum & Marshall, 1984), agarose (Khare, Jha, & Gandhi, 1994), cellulose carriers (Fujii et al, 1994;Sankpal et al, 2001), or attachment on cotton fibres, metal screens, polyester felts (Liu, Li, Ridgway, Gu, & Moo-Young, 1998;Papagianni, Joshi, & Moo-Young, 2002).…”

Physiological aspects of free and immobilized Aspergillus niger cultures producing citric acid under various glucose concentrations

“…Several studies report the use of Aspergillus niger immobilized on various kinds of solid supports, e.g. glass carriers [16], polyurethane foams [17], entrapment in calcium alginate beads [1,18,19], polyacrylamide gels [20,21], agar [22], agarose [23], cellulose carriers [2,3], or attachment on cotton fibres, metal screens, polyester felts [24,25]. Several attempts have also been reported on the production of citric acid by A. niger grown immobilized in surface cultures, as well as biomass recycle processes, where A. niger biomass was repeatedly used, by means of medium replacement.…”

Studies on citric acid production with immobilized Yarrowia lipolytica in repeated batch and continuous air-lift bioreactors

“…The same year Briffaud and Engasser (1978) reported on citric acid production by Saccharomycopsis lipolytica yeast grown on beechwood shavings in a trickle-filter reactor after an ammonia-activated growth phase. Vaija et al (1982) used a continuous air-lift-type fermentor for citric acid production with calcium-alginate-immobilized A. niger. Small quantities of ammonium nitrate appear to be necessary for the maximum yield and productivity with immobilized A. niger (Linko and Linko 1984).…”