‘Citricoccus massiliensis’ sp. nov., a new bacterial species isolated from human skin by culturomics

Ndiaye, Codou; Basséne, Hubert; Cadoret, F.; Raoult, Didier; Lagier, Jean-Christophe; Sokhna, Cheikh

doi:10.1016/j.nmni.2018.02.004

Cited by 3 publications

(1 citation statement)

References 6 publications

(6 reference statements)

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“…was of an isolate from the Cuatro Cienegas Basin (CCB) in Coahuila, Mexico (2). The members of this genus have been isolated and reported from various environments, such as marine macroalgae (3), mold-colonized walls (4), saline wastewater bioreactors (5), wastewater treatment plants (6), medieval wall paintings (1), desert soil (7), and human skin (8). There are no previous reports indicating the presence of Citricoccus spp.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Citricoccus sp. Strain SGAir0253, Isolated from Indoor Air in Singapore

Chandrasekaran

Drautz–Moses

Uchida

et al. 2019

Microbiol Resour Announc

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Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Citricoccus sp. Strain SGAir0253, Isolated from Indoor Air in Singapore

Chandrasekaran

Drautz–Moses

Uchida

et al. 2019

Microbiol Resour Announc

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Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory

Cerutti²,

et al. 2020

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SUMMARY This review provides a state-of-the-art description of the performance of Sanger cycle sequencing of the 16S rRNA gene for routine identification of bacteria in the clinical microbiology laboratory. A detailed description of the technology and current methodology is outlined with a major focus on proper data analyses and interpretation of sequences. The remainder of the article is focused on a comprehensive evaluation of the application of this method for identification of bacterial pathogens based on analyses of 16S multialignment sequences. In particular, the existing limitations of similarity within 16S for genus- and species-level differentiation of clinically relevant pathogens and the lack of sequence data currently available in public databases is highlighted. A multiyear experience is described of a large regional clinical microbiology service with direct 16S broad-range PCR followed by cycle sequencing for direct detection of pathogens in appropriate clinical samples. The ability of proteomics (matrix-assisted desorption ionization-time of flight) versus 16S sequencing for bacterial identification and genotyping is compared. Finally, the potential for whole-genome analysis by next-generation sequencing (NGS) to replace 16S sequencing for routine diagnostic use is presented for several applications, including the barriers that must be overcome to fully implement newer genomic methods in clinical microbiology. A future challenge for large clinical, reference, and research laboratories, as well as for industry, will be the translation of vast amounts of accrued NGS microbial data into convenient algorithm testing schemes for various applications (i.e., microbial identification, genotyping, and metagenomics and microbiome analyses) so that clinically relevant information can be reported to physicians in a format that is understood and actionable. These challenges will not be faced by clinical microbiologists alone but by every scientist involved in a domain where natural diversity of genes and gene sequences plays a critical role in disease, health, pathogenicity, epidemiology, and other aspects of life-forms. Overcoming these challenges will require global multidisciplinary efforts across fields that do not normally interact with the clinical arena to make vast amounts of sequencing data clinically interpretable and actionable at the bedside.

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Correction: Asymptomatic carriage of Streptococcus pneumoniae detected by qPCR on the palm of hands of populations in rural Senegal

2024

PLoS Negl Trop Dis

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‘Citricoccus massiliensis’ sp. nov., a new bacterial species isolated from human skin by culturomics

Abstract: We report the main characteristics of ‘Citricoccus massiliensis’strain Marseille-P4330, a new species within the genus Citricoccus. This strain was isolated from the skin of a healthy human man living in Dielmo, Senegal, Western Africa.

Cited by 3 publications

References 6 publications

Complete Genome Sequence of Citricoccus sp. Strain SGAir0253, Isolated from Indoor Air in Singapore

Complete Genome Sequence of Citricoccus sp. Strain SGAir0253, Isolated from Indoor Air in Singapore

Performance and Application of 16S rRNA Gene Cycle Sequencing for Routine Identification of Bacteria in the Clinical Microbiology Laboratory

Correction: Asymptomatic carriage of Streptococcus pneumoniae detected by qPCR on the palm of hands of populations in rural Senegal

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