2005
DOI: 10.1016/j.mcp.2004.11.002
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Citrus huanglongbing in São Paulo State, Brazil: PCR detection of the ‘Candidatus’ Liberibacter species associated with the disease

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Cited by 222 publications
(67 citation statements)
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“…There are only a few publications that analyzed the bacterial communities in citrus samples by PCR methods. Most of the work was done on fruit pathogen identification on strains such as Candidatus liberibacter by PCR [23][24][25] or real-time PCR [26], Methylobacterium mesophilicum [27], Xanthomonas axonopodis pv. citri [28] and Xylella fastidiosa [29] but no references relate to the DGGE method.…”
Section: Discussionmentioning
confidence: 99%
“…There are only a few publications that analyzed the bacterial communities in citrus samples by PCR methods. Most of the work was done on fruit pathogen identification on strains such as Candidatus liberibacter by PCR [23][24][25] or real-time PCR [26], Methylobacterium mesophilicum [27], Xanthomonas axonopodis pv. citri [28] and Xylella fastidiosa [29] but no references relate to the DGGE method.…”
Section: Discussionmentioning
confidence: 99%
“…The first is based on 16S rDNA sequence, using many sequences of primers and probes (Fujikawa and Iwanami 2012). Primer pair OI1 and OI2c is able to amplify the rDNA Las and Laf species (Teixeira et al 2005a). When the 2 Ca.…”
Section: Molecular Techniques For Hlb Detectionmentioning
confidence: 99%
“…Sequence analysis shows that the 16S rDNA amplified from Las has one XbaI restriction site and yields, upon XbaI treatment, 2 fragments of sizes 520 and 640 bp. The 16S rDNA amplified from Laf has an additional restriction site and yields 3 fragments of 520, 506, and 130 bp (Jagoueix et al 1996;Teixeira et al 2005a). The second PCR is centered on the nusG-rplK operon region (primer pair A2 and J5, and primer pair MHO353 and MHO354) (Fujikawa and Iwanami 2012).…”
Section: Molecular Techniques For Hlb Detectionmentioning
confidence: 99%
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“…Cirus huanglongbing (HLB) is the destructive a-proteobacteria disease of citrus in Asia, Africa, Arabic Peninsular, Mauritius Island, Madagascar (Garnier and Bove,1996) and recently in America and Brazil (Teixeira et al, 2005). Being an important disease, it affects production of citrus in several parts of Malaysia including Terengganu, which is the major producer area of citrus in Malaysia.…”
Section: Introductionmentioning
confidence: 99%