Citrus viroid II variants associated with ?Gummy Bark? disease

Önelge, N.; Çınar, Ahmet; Szychowski, J. A.; Vidalakis, Georgios; Semancik, J. S.

doi:10.1007/s10658-004-0815-2

Cited by 20 publications

(15 citation statements)

References 7 publications

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“…Partially replacing sole R or B or R/B mixture by green light did not cause differences in leaf area, SLA, shoot: root ratio and biomass of MM and cry1a mutant. This contradicts previous ndings on green light responses, but in those studies PPFD also increased when adding G (Kim, 2005; Samuolienė et al, 2012; Novičkovas at al., 2012). Zhang et al (2011) reported that 40% green light induced a shade avoidance response in Arabidopsis seedlings, whereas 10% did not.…”

Section: Phys Play Role In Blue Light Effects On Elongationcontrasting

confidence: 73%

“…Higher G intensity also increased contents of photosynthetic pigments in Arabidopsis seedlings, and biomass and photosynthetic parameters in leaves of lettuce (Golovatskaya and Karnachuk, 2008;E mova et al, 2013;Johkan et al, 2012;Muneer et al, 2014). Lettuce plants grown in a mixture of R, B and G (RBG) had larger speci c leaf area (SLA) but lower stomatal conductance (gs) compared with RB alone, where the total light intensity of RBG was higher than that of RB (Kim, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Green light reduces elongation when partially replacing sole blue light independently from cryptochrome 1a

Zhang

Bisbis

Heuvelink

et al. 2020

Preprint

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Although green light is often neglected it can have several effects on plant growth and development. Green light is probably sensed by cryptochromes (crys), one of the blue light photoreceptor families. The aim of this study is to investigate the possible interaction between green and blue light and the involvement of crys in the green light response of plant photomorphogenesis. We hypothesize that green light effects on morphology only occur when crys are activated by the presence of blue light. Wild-type Moneymaker (MM), cry1a mutant (cry1a) and two CRY2 overexpressing transgenic lines (CRY2-OX3 and CRY2-OX8) of tomato (Solanum lycopersicum) were grown in a climate chamber without or with green light (30 µmol m− 2 s− 1) on backgrounds of sole red, sole blue and red/blue mixture, with all treatments having the same photosynthetic photon flux density of 150 µmol m− 2 s− 1. Green light showed no significant effect on biomass accumulation, nor on leaf photosynthesis and leaf characteristics such as leaf area, specific leaf area, and chlorophyll content. However, in all genotypes, green light significantly decreased stem length on a sole blue background, whereas green light did not affect stem length on sole red and red/blue mixture background. MM, cry1a and CRY2-OX3/8 plants all exhibited similar responses of stem elongation to green light, indicating that cry1a, and probably cry2, is not involved in this green light effect. We conclude that partially replacing blue light by green light reduces elongation and that this is independent of cry1a.

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Section: Phys Play Role In Blue Light Effects On Elongationcontrasting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Green light reduces elongation when partially replacing sole blue light independently from cryptochrome 1a

Zhang

Bisbis

Heuvelink

et al. 2020

Preprint

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“…Sequencing confirmed that Ca‐909 lacks the ‘cachexia cluster’ (Reanwarakorn and Semancik 1999). Furthermore, population profiles and genetic lineages also indicate that the Ca‐909 variant may not be a cachexia disease agent (Önelge et al. 2004; Vidalakis et al.…”

Section: Introductionmentioning

confidence: 99%

Molecular, Biological and Phylogenetic Analysis of Chinese Isolates ofHop stunt viroidAssociated with Citrus Cachexia Disease

Wang

Zhou

et al. 2010

Journal of Phytopathology

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Citrus cachexia is an economically important disease of citrus hosts caused by specific variants of Hop stunt viroid (HSVd) that are usually referred to as Citrus cachexia viroid (CCaVd). Eight cachexia-associated HSVd isolates were collected from six citrus growing areas of China, where citrus cachexia had not been reported previously. Forty-seven independent cDNA clones were used for genetic diversity and phylogenetic analysis. There were no sequence variant-cultivar correlation and no distinct regional specificity among or within the cachexia-associated HSVd populations analysed. Three clusters consisting of three major HSVd variants were identified by phylogenetic analysis, suggesting that most Chinese isolates contain a mixture of cachexia and non-cachexia variants. Biological properties of eight CCaVd isolates were determined by inoculating ParsonÕs Special mandarin (Citrus reticulata). Our results suggest that the interactions between CCaVd and non-CCaVd variants might play an important role in suppressing cachexia symptom expression.

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“…Multiplex RT-qPCR has been reported to be a robust and economical detection tool for a broad range of different plant pathogens ( Genetic variability within a pathogen species is often a drawback in the design of primers and MGB probes for qPCR. Despite their small genome, genetic variability within viroid species has been reported for different hosts and geographical areas (Amari et al, 2001;Fadda et al, 2003;Flores et al, 2005;Ito et al, 2002;Lin et al, 2015;Mohamed et al, 2009;Önelge et al, 2004;Owens, 2008;Semancik et al, 1994;Semancik and Duran-Vila 1999;Szychowski et al, 2005;Wang et al, 2013). Therefore, all available CEVd, CBCVd, and HSVd genome sequences in GenBank were used to design the assays, and a wide range of samples from different hosts and geographical areas were used to validate and evaluate the assays.…”

Section: Discussionmentioning

confidence: 99%

One-step multiplex RT-qPCR detects three citrus viroids from different genera in a wide range of hosts

Osman

Dang

Bodaghi

et al. 2017

Journal of Virological Methods

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A one-step multiplex reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR) based on species-specific minor groove binding (MGB) probes, was developed for the simultaneous detection, identification, and quantification of three citrus viroids belonging to different genera. Citrus exocortis viroid (Pospiviroid), Hop stunt viroid (Hostuviroid), and Citrus bark cracking viroid (Cocadviroid) cause a variety of maladies in agriculturally significant crops. Therefore, reliable assays for their detection are essential tools for various government and industry organizations implementing disease management programs. Singleplex qPCR primers and MGB probes were designed individually for the detection of the three targeted viroids, and subsequently combined in a one-step multiplex RT-qPCR reaction. A wide host range of woody plants, including citrus, grapevines, apricots, plums and herbaceous plants such as tomato, cucumber, eggplant and chrysanthemum different world regions were used to validate the assay. Single, double and triple viroid infections were identified in the tested samples. The developed multiplex RT-qPCR assay was compared with a previously reported SYBR Green I RT-qPCR for the universal detection of citrus viroids. Both assays accurately identified all citrus viroid infected samples. The multiplex assay complemented the SYBR Green I universal detection assay by differentiating among citrus viroid species in the positive samples. The developed multiplex RT-qPCR assay has the potential to simultaneously detect each targeted viroid and could be used in high throughput screenings for citrus viroids in field surveys, germplasm banks, nurseries and other viroid disease management programs.

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Citrus viroid II variants associated with ?Gummy Bark? disease

Cited by 20 publications

References 7 publications

Green light reduces elongation when partially replacing sole blue light independently from cryptochrome 1a

Green light reduces elongation when partially replacing sole blue light independently from cryptochrome 1a

Molecular, Biological and Phylogenetic Analysis of Chinese Isolates ofHop stunt viroidAssociated with Citrus Cachexia Disease

One-step multiplex RT-qPCR detects three citrus viroids from different genera in a wide range of hosts

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