CJD and intraocular surgery

Osborne, Rowland

doi:10.1038/sj.eye.6701401

Cited by 1 publication

(1 citation statement)

References 9 publications

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“…No single standard yet exists for “acceptable” protein levels on reprocessed instruments. The BS EN ISO‐15883‐1: 2006 for validation of washer disinfectors defines an acceptable level as below the detection limit of one of three protein assays which are stated as 2 mg/m 2 for the Ninhydrin assay, 30 ‐ 50 μg for the bicinchoninic acid assay, and 0.003 μmol of OPA sensitive amino groups for the OPA assay [12]. Work undertaken by Lipscomb and colleagues (2006) also determined the threshold of sensitivity for similar reagents to be equivalent to 9.25 μg/10 mm 2 for Ninhydrin and 6.7 μg/10 mm 2 for the Biuret test [13].…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of residual protein contamination of podiatry instruments reprocessed through local and central decontamination units

Smith

Goldie²,

Long

et al. 2011

Journal of Foot and Ankle Research

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BackgroundThe cleaning stage of the instrument decontamination process has come under increased scrutiny due to the increasing complexity of surgical instruments and the adverse affects of residual protein contamination on surgical instruments. Instruments used in the podiatry field have a complex surface topography and are exposed to a wide range of biological contamination. Currently, podiatry instruments are reprocessed locally within surgeries while national strategies are favouring a move toward reprocessing in central facilities. The aim of this study was to determine the efficacy of local and central reprocessing on podiatry instruments by measuring residual protein contamination of instruments reprocessed by both methods.MethodsThe residual protein of 189 instruments reprocessed centrally and 189 instruments reprocessed locally was determined using a fluorescent assay based on the reaction of proteins with o-phthaldialdehyde/sodium 2-mercaptoethanesulfonate.ResultsResidual protein was detected on 72% (n = 136) of instruments reprocessed centrally and 90% (n = 170) of instruments reprocessed locally. Significantly less protein (p < 0.001) was recovered from instruments reprocessed centrally (median 20.62 μg, range 0 - 5705 μg) than local reprocessing (median 111.9 μg, range 0 - 6344 μg).ConclusionsOverall, the results show the superiority of central reprocessing for complex podiatry instruments when protein contamination is considered, though no significant difference was found in residual protein between local decontamination unit and central decontamination unit processes for Blacks files. Further research is needed to undertake qualitative identification of protein contamination to identify any cross contamination risks and a standard for acceptable residual protein contamination applicable to different instruments and specialities should be considered as a matter of urgency.

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Section: Discussionmentioning

confidence: 99%