Quantitative analysis of residual protein contamination of podiatry instruments reprocessed through local and central decontamination units

Smith, Gardner W.; Goldie, Frank; Long, Steven; Lappin, David F.; Ramage, Gordon; Smith, Andrew

doi:10.1186/1757-1146-4-2

Cited by 11 publications

(10 citation statements)

References 17 publications

(24 reference statements)

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“…Acceptable protein levels on processed instruments have to be below the detection limit of 2 mg/m 2 for the ninhydrin assay, 30–50 μ g for the BCA assay, or 0.003 μ mol for the OPA assay [8]. However, to detect lower levels of contaminants, techniques with greater quantitative sensitivity are necessary.…”

Section: Discussionmentioning

confidence: 99%

Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Wilhelm

Perle

Simmoteit³

et al. 2014

BioMed Research International

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Surgical instruments are often strongly contaminated with patients' blood and tissues, possibly containing pathogens. The reuse of contaminated instruments without adequate cleaning and sterilization can cause postoperative inflammation and the transmission of infectious diseases from one patient to another. Thus, based on the stringent sterility requirements, the development of highly efficient, validated cleaning processes is necessary. Here, we use for the first time synthetic single-stranded DNA (ssDNA_ODN), which does not appear in nature, as a test soiling to evaluate the cleaning efficiency of routine washing processes. Stainless steel test objects were coated with a certain amount of ssDNA_ODN. After cleaning, the amount of residual ssDNA_ODN on the test objects was determined using quantitative real-time PCR. The established method is highly specific and sensitive, with a detection limit of 20 fg, and enables the determination of the cleaning efficiency of medical cleaning processes under different conditions to obtain optimal settings for the effective cleaning and sterilization of instruments. The use of this highly sensitive method for the validation of cleaning processes can prevent, to a significant extent, the insufficient cleaning of surgical instruments and thus the transmission of pathogens to patients.

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Section: Discussionmentioning

confidence: 99%

Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Wilhelm

Perle

Simmoteit³

et al. 2014

BioMed Research International

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“…Instrument reprocessing involves steps prior to sterilization, such as cleaning and disinfection. Improper reprocessing can lead to contamination at any stage of the process (Smith, 2011;Funk, 2014 (Amaha, 1954;Piroth, 2008). Moreover, not all cleaning agents inactivate prions (McDonnell, 2013).…”

Section: Instrument Reprocessing In Veterinary and Human Medicinementioning

confidence: 99%

Effects of repeated use and resterilization on structural and functional integrity of microwave ablation antennas

Finck¹,

Linden²,

Singh³

et al. 2017

ajvr

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OBJECTIVE To determine effects of repeated use and resterilization on structural and functional integrity of microwave ablation (MWA) antennas. SAMPLE 17 cooled-shaft MWA antennas (3 groups of 5 antennas/group and 2 control antennas). PROCEDURES 1, 2, and 3 ablations in the livers of bovine cadavers were performed at the maximum recommended settings. Antennas were cleaned and sterilized in hydrogen peroxide plasma, and the process was repeated (reprocessing cycle; n = 6). Control antennas were only sterilized (6 times). Aerobic and anaerobic bacterial cultures were performed, and antennas were microscopically assessed for damage. RESULTS 6 cycles were completed. Thirteen of 15 MWA antennas remained functional for up to 4 cycles, 10 were functional after 5 cycles, and only 7 were functional after 6 cycles. Progressive tearing of the silicone coating of the antennas was observed, with a negative effect of the number of cycles for silicone tearing. Size of the ablation zone decreased mildly over time after cycles 5 and 6; however, this was not considered clinically relevant. No significant changes in the shape of ablation zones were detected. All cultures yielded negative results, except for an isolated case, which was considered a contaminant. CONCLUSIONS AND CLINICAL RELEVANCE Structural and functional integrity of the microwave antennas remained acceptable during repeated use and reprocessing for up to 4 cycles. However, there was a decrease in functional integrity at cycles 5 and 6. We suggest that these microwave antennas be subjected to > 3 reprocessing cycles. Antennas should be carefully examined before reuse.

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“…This process is guided by professional evidenced‐based guidelines and by the instrument manufacturer's instructions for use 44 . Inadequately reprocessed sterile (critical) instruments have been associated with surgical infection 45‐47 . A strong relationship between SP professionals and IPs can facilitate safe patient care.…”

Section: Ambulatory Surgery Centersmentioning

confidence: 99%

Infection Prevention: The Surgical Care Continuum

Barnes¹

2015

AORN Journal

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Lack of careful attention to the increasing complexity of surgical procedures, instrument cleaning and processing, and the transition of surgical and other invasive procedures from the OR to areas outside the traditional hospital OR can contribute to surgical site infection (SSI) risk. Regardless of the location of an intervention, when basic infection prevention measures are applied reliably, even low infection rates can be reduced. To address infection prevention challenges, infection preventionists (IPs) must be well informed regarding infection risk and prevention during surgical and other invasive procedures and the effect a facility type may have on patients' infection risk. The IP must have a solid understanding of surgical asepsis, instrument disinfection, and sterilization to educate and support clinical teams in identifying opportunities for improvement relative to infection prevention.

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Quantitative analysis of residual protein contamination of podiatry instruments reprocessed through local and central decontamination units

Cited by 11 publications

References 17 publications

Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Use of Synthetic Single-Stranded Oligonucleotides as Artificial Test Soiling for Validation of Surgical Instrument Cleaning Processes

Effects of repeated use and resterilization on structural and functional integrity of microwave ablation antennas

Infection Prevention: The Surgical Care Continuum

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