Cladosporium Species-Related Hypersensitivity Pneumonitis in Household Environments

Chiba, Shigeki; Okada, Shinji; Suzuki, Yasuko; Watanuki, Zenta; Mitsuishi, Yoichiro; Igusa, Ryotaro; Sekii, Takehiko; Uchiyama, Bine

doi:10.2169/internalmedicine.48.1811

Cited by 34 publications

(15 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cladosporium spp. is the common and widespread fungi on land and in air [36, 37]. Although there are no publications regarding its ability of producing toxin, they do produce odours likely relating to some volatile organic compounds.…”

Section: Resultsmentioning

confidence: 99%

An investigation of fungal contamination on the surface of medicinal herbs in China

et al. 2017

View full text Add to dashboard Cite

BackgroundThe dried parts of medicinal herbs are susceptible to the infection of fungi during pre- or post-harvest procedure. This study aimed to investigate the presence of fungi and their metabolites mycotoxins on the surface of medicinal herbs collected from China.MethodsForty-five retail samples of 15 different medicinal herbs were collected from 3 different regions in China. Then the potential fungi were immediately washed off from the surface of each sample with 0.1% Tween-20 followed by incubation of the rinse on petri-dish with potato dextrose agar containing chloramphenicol at 28 °C. The obtained fungi were isolated as single colonies and then characterized by morphology and molecular identification using internal transcribed spacer (ITS) sequencing with extracted DNA. Meanwhile, the mycotoxin-producing potential of the isolates was studied by liquid chromatography-tandem mass spectrometry (LC-MS/MS).ResultsA total of 126 fungi were identified from the surface of samples by morphology and ITS sequencing, with Aspergillus and Penicillium genera as the predominant contaminants. The mycotoxin-producing potential analysis showed that 6 of 8 A. versicolor isolates could produce sterigmatocystin. All 3 A. aculeatus isolates produced ochratoxin A, but only 1 of 3 A. flavus strains produced aflatoxins B1 and B2 without G1 and G2. Although the sample contamination ratios were high (≥95.6%), there was no significant difference (χ 2 = 1.05, P = 1.0) among the samples from 3 regions, which demonstrates the prevalent fungal contamination in the herbal medicines.ConclusionThe prevalent contamination phenomenon of fungi and high potential risk of sterigmatocystin and ochratoxin A were observed in 45 medicinal herbs collected from China.Electronic supplementary materialThe online version of this article (doi:10.1186/s13020-016-0124-7) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultsmentioning

confidence: 99%

An investigation of fungal contamination on the surface of medicinal herbs in China

et al. 2017

View full text Add to dashboard Cite

show abstract

“…A significant relationship was found between the reduction in lung function and airborne mold concentrations (Dahlqvist et al 1992). Respiratory symptoms, febrile episodes, allergic diseases such as hypersensitivity pneumonitis, and asthma due to bioaerosol exposure have been documented among workers employed in waste industry (Chiba et al 2009;Poulsen et al 1995). It was also found that not only viable, but also non-viable bioaerosol particles are capable of causing adverse health effects (Adhikari et al 2009;Gorny et al 2002;Robbins et al 2000).…”

Section: Introductionmentioning

confidence: 97%

Performance of an Electrostatic Precipitator with Superhydrophobic Surface when Collecting Airborne Bacteria

Han

Mainelis

2010

Aerosol Science and Technology

View full text Add to dashboard Cite

Modern bioaerosol sampling and analysis techniques that enable rapid detection of low bioagent concentrations in various environments are needed to help us understand the causal relationship between adverse health effects and bioaerosol exposures and also to enable the timely biohazard detection in case of intentional release.We have developed a novel bioaerosol sampler, an electrostatic precipitator with superhydrophobic surface (EPSS), where a combination of electrostatic collection mechanism with superhydrophobic collection surface allows for efficient particle collection, removal, and concentration in water droplets as small as 5 µL. The sampler's performance at different sampling flow rates and sampling times was tested with two common bacteria: Pseudomonas fluorescens and Bacillus subtilis. The collection efficiency was determined using the traditional method of microscopic counting as well as the whole-cell quantitative real-time polymerase chain reaction assay (QPCR). The tests indicated that the new sampler achieves collection efficiency as high as 72%. A combination of the satisfactory collection efficiency and the small collecting water droplet volumes allowed achieving sample concentration rates that exceed 1 × 10 6 . In addition, the collection efficiency for both bacteria obtained by the two different methods was not statistically different, indicating the sampler's compatibility with the PCR-based sample analysis techniques. In addition, the whole-cell QPCR does not require DNA extraction prior to the PCR reaction which offers faster sample processing.Very high concentration rates achieved with the new sampler as well as its compatibility with the QPCR methodology point toward its suitability for detecting low concentrations of airborne bacterial agents in various environments.

show abstract

“…There are many ILDs with different etiologies and histological manifestations, and histological manifestation does not correspond to etiology according to a one-to-one relation (Scott et al 1990;Baumgartner 2000;Miyake 2005). In addition, HP cases indistinguishable from IIPs have recently been recognized (Chiba et al 2009). Thus, a diagnosis system for the etiological factors is needed which involves lower invasiveness.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we reported a patient with chronic HP caused by Cladosporium species that developed in a household environment, but not in specific environments (Chiba et al 2009). Although several coincidences helped us to reach a diagnosis of HP in this case after several years of follow up, a large number of patients with HP can be misdiagnosed as IIPs.…”

mentioning

confidence: 98%

Increased Prevalence of High Anti-<i>Cladosporium</i> Antibody Titers in Interstitial Lung Diseases

Watanuki

Okada

Chiba

et al. 2012

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

Interstitial lung diseases (ILDs) represent a large group of different diseases, with a large part comprising idiopathic interstitial pneumonias. Differentiating hypersensitivity pneumonitis (HP), especially its chronic form and other ILDs, is difficult because of similarities in radiological manifestation and clinical course, and the difficulty of identifying causative antigens. We recently experienced a patient with Cladosporiuminduced chronic HP that developed in a household environment, but the cause had been misdiagnosed as idiopathic interstitial pneumonia for several years. This case highlighted the need for measures differentiating HP from idiopathic interstitial pneumonia. In this study, we examined fungal exposure in ILDs using an antibody titer in serum to identify possible fungus-related HP. We measured the antibody titer to Cladosporium spp. in 34 patients with various ILDs, 17 patients with bronchial asthma, and 21 control subjects using an immunofluorescence assay. ILDs included HP (5 patients), idiopathic interstitial pneumonias (21 patients), and ILDs with collagen vascular diseases (8 patients). Results showed a significantly higher tendency for high anti-Cladosporium antibody titers in ILD groups (12 patients out of 34 patients), compared to patients with bronchial asthma (0/17) or control subjects (0/21). This increase in antibody titers was observed not only in patients with HP, but also in those with idiopathic interstitial pneumonias and those exhibiting collagen vascular diseases with ILDs. This report highlights the pathogenic role of fungal antigens in various ILDs. In conclusion, fungi commonly observed in our living environment such as Cladosporium could be involved in the development of ILDs.

show abstract

Cladosporium Species-Related Hypersensitivity Pneumonitis in Household Environments

Abstract: Home-related chronic hypersensitivity pneumonitis (HP)

Cited by 34 publications

References 7 publications

An investigation of fungal contamination on the surface of medicinal herbs in China

An investigation of fungal contamination on the surface of medicinal herbs in China

Performance of an Electrostatic Precipitator with Superhydrophobic Surface when Collecting Airborne Bacteria

Increased Prevalence of High Anti-<i>Cladosporium</i> Antibody Titers in Interstitial Lung Diseases

Contact Info

Product

Resources

About