Class 1 Integrons and Antibiotic Resistance of Clinical Acinetobacter calcoaceticus–baumannii Complex in Poznań, Poland

Background: Acinetobacter baumannii is a significant nosocomial pathogen around the world, especially in the intensive care unit that most A. baumannii infections are caused by the outbreak strains. Objectives: This study has been performed in Acinetobacter baumannii isolates, aimed to detect integron classes I, II, III and molecular typing of A. baumannii genes. Methods: In this Cross-sectional study, Acinetobacter baumannii isolated from 150 patients in Isfahan hospitals then antibiotic resistance pattern was determined by disk diffusion method (Kirby Bauer). The presence of genes coding in antibiotic resistance and integrons class I, II, III were analyzed by using of M-PCR method. The data were analyzed by Chi-square, Fischer's test and SPSS statistical software version 16. Results: Antibiotic resistance pattern for Acinetobacter baumannii show that the high resistance was for ciprofloxacin with frequency of 98.3%, ceftazidime with 89.4%, and tetracycline with frequency of 87.3%. The most sensitive antibiotics were chloramphenicol, and nitrofurantoin with frequency of 3.5% and 3.2% resistance. The detection of dfrA1 (63.7%), sul1 (68.6%), aac (3)-IV (54.4%), tet (B) (22.4%), tet (A) (78.3%), aadA1 (15.4%), CITM (17. %), vim (12.2%), Qnr (17.1%), blaSHV (19.8%), sim (7.8%), Oxa-24-like (13.2%), Oxa-51-like (11.9%), Oxa-58-like (39.4%), Oxa-23-like (12.6%), imp (9.2%), cmlA (19%) and cat1 (8.6%) were respectively reported too. Also in this study Frequency of integrons class 1, 2, 3 were (100%), (28%), (6.6%) respectively. Conclusions: High prevalence of integrons among Acinetobater baumannii isolated from Isfahan hospitals indicate the importance role of integron classes in multidrug resistance. Considering the increasing pattern of MDR infections is one of the important issues of treatment which can be effective strategy for curing.

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“…Similar to this study in Turkey (2014) only 33% of the isolates of the bacteria carry Integrons class I, and class II [32].…”

Section: Discussionsupporting

confidence: 87%

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Nourbakhsh

Rajai

Momtaz

2017

Zahedan J Res Med Sci

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“…Over the past decades, regardless of new therapeutic options, A. baumannii strains have shown a remarkable ability to rapid development of multi-drug resistance (MDR). This rapid increase of MDR is not only due to the intrinsic resistant genes carried by these strains, but also to their outstanding capacity to acquire resistant elements from other bacteria (2).…”

Section: Introductionmentioning

confidence: 99%

Dissemination of Classes 1, 2, and 3 Integrons in Acinetobacter baumannii Strains Recovered from Intensive Care Units Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

Goudarzi

Hadi

2017

Jundishapur J Microbiol

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Background: Acinetobacter baumannii as the most important nosocomial pathogen can cause various types of clinical infections. Multidrug resistance in A. baumannii strains has significantly limited the choice of available therapeutic options for treatment of patients and is a serious threat to hospitalized patients, especially those in intensive care units. Objectives: The present study was performed to investigate the frequency of classes 1, 2, and 3 integrons in A. baumannii strains isolated from selected hospital ICUs. Methods: A total of 105 A. baumannii isolates were collected between October 2015 and April 2016 from 4 medical centers located in different regions of Tehran, Iran. The resistance rate to different classes of antimicrobial agents was assessed using Epsilometer test. Conventional PCR was performed for the detection of blaOXA-51-like gene and integrase gene. Restriction Fragment Length Polymorphism technique using RsaI and HinfI restriction enzymes was used to detect integron classes. Results: All the isolates were observed to be susceptible to colistin and polymixin B and inhibited at similar minimum inhibitory

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“…Bowman was characterized as demonstrating nonsusceptibility to at least 1 agent in all but 2 or fewer antimicrobial classes. The genes encoding aminoglycoside modifying enzymes may be located on transposons, plasmids or class 1 integrons in MDR A. baumannii strains in Europe (12)(13)(14). These genes include nucleotidyltransferases (aadB), ANT (3") -Ia (aadA1), phosphotransferases (aphA6), and acetyltransferases (aacC1), which were detected by PCR and sequencing.…”

Section: Introductionmentioning

confidence: 99%

Distribution of Aminoglycoside Resistance Genes Among Acinetobacter Baumannii Strains Isolated From Burn Patients in Tehran, Iran

Khoshnood

Eslami

Hashemi

et al. 2017

Arch Pediatr Infect Dis

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Background: Acinetobacter baumannii is an important cause of nosocomial infections, particularly in burn patients. Hospital Infections caused by these bacteria are difficult to treat. Objectives: The present study aimed at determining the frequency of genes encoding aminoglycoside-modifying enzymes in A. baumannii strains isolated from burn patients in Shahid Motahari hospital. Methods: This study was performed on 100 A. baumannii strains collected from Shahid Motahari hospital in Tehran during 2013 and 2014. The bacteria were cultured and identified. Antibiotic susceptibility testing was performed by disk diffusion method according to the CLSI guidelines. PCR assay was done to find the genes encoding aminoglycoside-modifying enzymes. Results: In this study, highest resistance to antibiotic was reported for ceftriaxone, ciprofloxacin, and ceftizoxime (100%), whereas the highest susceptibility was observed for colistin (100%), followed by gentamicin, amikacin, and tobramycin with (93%), (90%), and (87%), respectively. In the total of 100 strains studied, aphA6, aadB, aacC1 and aadA1 genes were found in 657 221 and 37 of A. baumannii isolates, respectively; 8 isolates had aadB and aphA6 genes and 3 had aadB, aadA1, aacC1, and aphA6 genes. Conclusions: This study showed the high frequency aminoglycoside-resistance genes among A. baumanni strains. Thus, the implementation of appropriate programs to prevent the spread of the bacteria seems necessary in the Shahid Motahari hospital.

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Class 1 Integrons and Antibiotic Resistance of Clinical Acinetobacter calcoaceticus–baumannii Complex in Poznań, Poland

Cited by 22 publications

References 37 publications

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Antibiotic Resistance and Carriage Integron Classes in Clinical Isolates of Acinetobacter Baumannii from Isfahan Hospitals, Iran

Dissemination of Classes 1, 2, and 3 Integrons in Acinetobacter baumannii Strains Recovered from Intensive Care Units Using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism

Distribution of Aminoglycoside Resistance Genes Among Acinetobacter Baumannii Strains Isolated From Burn Patients in Tehran, Iran

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