Class II biocompatible E-Shell 300 3D printing material causes severe developmental toxicity in <i>Danio rerio</i> embryos and reduced cell proliferation <i>in vitro</i> – implications for 3D printed microfluidics

Nejedlá, Zuzana; Poustka, David; Herma, Regina; Liegertová, Michaela; Štofik, Marcel; Šmejkal, Jiří; Šícha, Václav; Kaule, Pavel; Malý, Jan

doi:10.1039/d1ra00305d

Cited by 14 publications

(16 citation statements)

References 41 publications

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“…Despite the progress, some biocompatible 3D printing machines were proven to cause developmental toxicity in embryo culture, which was evidenced by Danio rerio embryo culture. 85 These findings demonstrate that there is still a long way to go before the extensive use of 3D printed device in developmental biology (Table 2). Organ-preserving surgery is a reasonable approach in managing a wide variety of penile disorders, such as penile cancer, trauma, and congenital anomalies.…”

Section: Matrix Mechanical Properties For Oocyte Maturationmentioning

confidence: 83%

“…A device with two stamps was designed and prepared for the increased injection and screening of embryos, 84 potentially acting as a feasible platform for clinical applications. Despite the progress, some biocompatible 3D printing machines were proven to cause developmental toxicity in embryo culture, which was evidenced by Danio rerio embryo culture 85 . These findings demonstrate that there is still a long way to go before the extensive use of 3D printed device in developmental biology (Table 2).…”

Section: Applications Of Biomedical Engineering Technologies In Infer...mentioning

confidence: 99%

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Developing biomedical engineering technologies for reproductive medicine

Zhu

Kong

et al. 2022

Smart Medicine

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Infertility is a rising global health issue with a far‐reaching impact on the socioeconomic livelihoods. As there are highly complex causes of male and female infertility, it is highly desired to promote and maintain reproductive health by the integration of advanced technologies. Biomedical engineering, a mature technology applied in the fields of biology and health care, has emerged as a powerful tool in the diagnosis and treatment of infertility. Nowadays, various promising biomedical engineering approaches are under investigation to address human infertility. Biomedical engineering approaches can not only improve our fundamental understanding of sperm and follicle development in bioengineered devices combined with microfabrication, biomaterials, and relevant cells, but also be applied to repair uterine, ovary, and cervicovaginal tissues and restore tissue function. Here, we introduce the infertility in male and female and provide a comprehensive summary of the various promising biomedical engineering technologies and their applications in reproductive medicine. Also, the challenges and prospects of biomedical engineering technologies for clinical transformation are discussed. We believe that this review will promote communications between engineers, biologists, and clinicians and potentially contribute to the clinical transformation of these innovative research works in the immediate future.

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Section: Matrix Mechanical Properties For Oocyte Maturationmentioning

confidence: 83%

Section: Applications Of Biomedical Engineering Technologies In Infer...mentioning

confidence: 99%

Developing biomedical engineering technologies for reproductive medicine

Zhu

Kong

et al. 2022

Smart Medicine

View full text Add to dashboard Cite

show abstract

“…25,26 Indeed, it gathers transparency, flexibility and peculiar chemical properties with good printability. On the other hand, it is well known that cells are influenced by the environment in which they live: 27,28 mechanical, chemical and physical material properties can all have a deep impact on cell behavior. 29 Consequently, each new material must be evaluated by cytotoxicity tests, which can manifest, for example, reduced cell viability, inflammatory response or DNA damage.…”

Section: Introductionmentioning

confidence: 99%

3D printable acrylate polydimethylsiloxane resins for cell culture and drug testing

et al. 2023

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“…Previous investigations on 3D printable photopolymers for biological applications focused on cell culture compatibility, − but little has been done to evaluate their inhibitory effects on in vitro enzymatic reactions. In vitro enzymatic reactions, such as PCR, are essential for lab-on-a-chip applications, where the transition from traditional microfabrication techniques to 3D printing has been longed for.…”

Section: Introductionmentioning

confidence: 99%

Compatibility of Popular Three-Dimensional Printed Microfluidics Materials with In Vitro Enzymatic Reactions

Lin¹,

Evenson

Bostic³

et al. 2022

ACS Appl. Bio Mater.

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3D printed microfluidics offer several advantages over conventional planar microfabrication techniques including fabrication of 3D microstructures, rapid prototyping, and inertness. While 3D printed materials have been studied for their biocompatibility in cell and tissue culture applications, their compatibility for in vitro biochemistry and molecular biology has not been systematically investigated. Here, we evaluate the compatibility of several common enzymatic reactions in the context of 3D-printed microfluidics: (1) polymerase chain reaction (PCR), (2) T7 in vitro transcription, (3) mammalian in vitro translation, and (4) reverse transcription. Surprisingly, all the materials tested significantly inhibit one or more of these in vitro enzymatic reactions. Inclusion of BSA mitigates only some of these inhibitory effects. Overall, inhibition appears to be due to a combination of the surface properties of the resins as well as soluble components (leachate) originating in the matrix.

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Class II biocompatible E-Shell 300 3D printing material causes severe developmental toxicity in Danio rerio embryos and reduced cell proliferation in vitro – implications for 3D printed microfluidics

Abstract: E-Shell 300 3D-printed material demonstrated a considerable negative impact on cell proliferation and severe developmental toxicity due to release of surfactant residues. Post-treatment with ethanol improved the biocompatibility of the material.

Cited by 14 publications

References 41 publications

Developing biomedical engineering technologies for reproductive medicine

Developing biomedical engineering technologies for reproductive medicine

3D printable acrylate polydimethylsiloxane resins for cell culture and drug testing

Compatibility of Popular Three-Dimensional Printed Microfluidics Materials with In Vitro Enzymatic Reactions

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