Classical coprological methods for diagnosing animal parasitosis

Arisov, M. V.; Panova, O. A.; Khrustalev, Alexander V.; Kurnosova, O. P.; Sysoeva, N.; Гламаздин, И Г

doi:10.31016/978-5-6048555-0-8.2022

2022

DOI: 10.31016/978-5-6048555-0-8.2022

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Classical coprological methods for diagnosing animal parasitosis

M. V. Arisov¹,

O. A. Panova²,

Alexander V. Khrustalev³

et al.

Abstract: учебно-методическом пособии изложены методы прижизненной диагностики паразитозов животных, а именно классические методы копрологической диагностики с учетом современных рекомендаций и предложений. Приведены основные возбудители разных видов животных, которые диагностируются при исследовании фекалий, их морфологические и дифференциально-диагностические параметры. Данное учебно-методическое пособие предназначено для студентов, обучающихся по направлению подготовки 36.05.01 – Ветеринария: специалистов, бакалавров… Show more

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2023

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Publishing Activities of the All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plants

Uspensky

Arisov

Архипов

et al. 2023

Proceedings of SPSTL SB RAS

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Since 2014, agricultural and medical scientific institutions, previously been a part of the specialized agricultural and medical academies of sciences are under the scientific and methodological guidance of the Russian Academy of Sciences (RAS). Among them is the All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plants – a branch of the Federal State Budget Scientific Institution “Federal Scientific Centre VIEV” (VNIIP – FSC VIEV), named after K. I. Skryabin and Ya. R. Kovalenko.Special attention in its scientific research is devoted to the study of formation and functioning patterns of parasitic systems; to the development of theoretical foundations to prevent parasitic diseases in livestock animals and plants, and to the improvement and implementation of integrated systems for protecting humans, animals and plants from socially dangerous and economically significant parasitic diseases by creating new methods for early diagnosis and prevention based on the widespread use of biotechnology, genetics and immunology; and to invention of fundamentally new means of protecting animals and plants that provide a high economic effect.The implementation of the Institute’s main tasks predetermines active scientific and publishing activities that can be designated as the final stage of any research work; therefore, scientific and research institutions publish monographs, collections of scientific papers, reference books, study guides, etc. The purpose of the article is to present main directions of VNIIP’s research and publishing activities highlighting features that characterize certain types of publications.The characteristic is given for two printed publications on advanced scientific research in parasitology, namely, the collection of materials from the “Theory and Practice of Parasitic Disease Control” Conference and the Russian Journal of Parasitology. A typological analysis of publications is given, and its results are presented. The declared features of the publications confirm their quality and meet key requirements of the current book publishing industry in this direction.The material is of interest to promote achievements and findings of the most important studies conducted by the team of scientists of the Institute and will be useful both to publishers and to authors of publications.

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Publishing Activities of the All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plants

Uspensky

Arisov

Архипов

et al. 2023

Proceedings of SPSTL SB RAS

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Efficacy of Bimectin® Anthelminthic against Helminthiasis in Horses in the Tyumen Region Conditions

Kalugina

Столбова²

2023

Vetpat

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Introduction. Helminthic infestation in horses is one of the most important problems the veterinary specialists face today, because helminth end-products affect significantly the overall condition of animals. The weight loss, depression, impaired work capacity, deterioration of stamina, exterior characteristics and natural resistance are observed and all together create the favourable conditions for arising the diseases of various etiology and cause considerable damage to horse breeding industry. The aim of the research is to determine the therapeutic efficacy of the anthelminthic drug «Bimectin®» against the intestinal helminthiasis in horses in the Tyumen region conditions.Materials and Methods. The studies were carried out with regard to the clinical examinations of animals and in compliance with the generally accepted parasitological methods. The infestation intensity (II), extensive efficacy (EE) and intensive efficacy (IE) were assessed, the haematological and biochemical blood tests were made on the 7th and 14th day before and after applying the drug. The results were statistically processed using the Statistica 6.1 software.Results. When assessing the therapeutic efficacy, we have determined that at a single application in the dose of 1.13 g. per 100 kg. of animal weight, the anthelminthic drug «Bimectin®» turned out to be 100 % therapeutically efficient and well tolerated by horses infested with Oxyuris equi and suborder Strongylata helminths, 91.7 % efficient against Parascaris equorum and 83.3 % efficient against Strongyloides westeri helminths.Discussion and Conclusions. Thus, in the result of assessing the therapeutic efficacy of the anthelmintic drug «Bimectin» against the horse helminthiasis, its economic benefit for using at the horse breeding farms of the region was confirmed, which should be taken into account when planning the combat measures against the nematode infestation in horses.

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Methods of coprological diagnostics of animal parasitoses

Panova,

Kurnosova,

Khrustalev

et al. 2023

Ross. parazitol. ž..

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The purpose of the research is to review the classical methods of coprological diagnostics, taking into account modern recommendations and proposals. Materials and methods. Literature sources on the issues of coprological methods of diagnosing parasitosis have been studied. Results and discussion. Diagnosis during life is an important element of practical veterinary activity, the success of which largely depends on the correct choice and competent use of suitable laboratory methods. For the diagnosis of parasitic diseases, the study of feces is most often used. Coprological methods are highly informative due to the fact that they can detect not only parasites of the digestive tract, liver, pancreas, but also a number of helminths of the respiratory system and even some tissue parasites that excrete eggs or larvae with feces. Unlike existing indirect immunological and molecular genetic methods, classical research methods are based on the detection and identification of the pathogens themselves at different stages of development. At the same time, the researcher has the opportunity to study morphological features and conduct morphometric analysis. The widespread use of classical coprological methods is also associated with their availability, they are quite simple to perform and relatively inexpensive. A limitation of coprological methods is the inability to diagnose pathogens that are at a non-propagative stage. The effectiveness of diagnostics is influenced by the method of collecting samples, the terms and conditions of transportation, and the choice of an adequate method for examining samples. The article presents the rules for sampling and options for the use of fixatives, a classification of coprological methods is given, and the procedure for performing research is described. Special attention is paid to the choice of flotation solutions, the method of cultivation of strongylid larvae and the method of sporulation of coccidia oocysts. Attention is drawn to the importance of micrometric measurements of detected objects. To ensure the quality of the conducted coprological studies, it is necessary to control the stages of the studies, the technical condition of the equipment and train the personnel. The material presented in the article is intended for both students and practitioners.

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Classical coprological methods for diagnosing animal parasitosis

Cited by 3 publications

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Publishing Activities of the All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plants

Publishing Activities of the All-Russian Scientific Research Institute for Fundamental and Applied Parasitology of Animals and Plants

Efficacy of Bimectin® Anthelminthic against Helminthiasis in Horses in the Tyumen Region Conditions

Methods of coprological diagnostics of animal parasitoses

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