Classical swine fever virus: independent induction of protective immunity by two structural glycoproteins

Abstract

To study which proteins of classical swine fever virus (CSFV) are able to confer protective immunity in swine, N-terminal autoprotease, viral core protein, and the three structural glycoproteins were expressed via vaccinia virus recombinants (VVR). CSFV proteins synthesized in cells infected with VVR showed migration characteristics on sodium dodecyl sulfate gels identical to those of their respective CSFV counterparts. Apparently authentic dimerization of the recombinant glycoproteins was observed. The glycoproteins E0 and E2 were detected on the surfaces of VVR-infected cells. In protection experiments, swine were immunized with the different VVR, and the generation of humoral immune response was monitored. Only animals vaccinated with VVR expressing E0 and/or E2 resisted a lethal challenge infection with CSFV. Glycoprotein E0 represents a second determinant for the induction of protective immunity against classical swine fever.

Role of disulfide bond formation in the folding and assembly of the envelope glycoproteins of a pestivirus

“…An important event in the assembly of pestiviruses is thought to be the formation of native E1-E2 heterodimers, the major components of two pestiviruses: the classical swine fever virus (CSFV) and BVDV [5,8,14]. These complexes begin their association through intermolecular disulfide bonds in the ER, after the monomers have acquired intramolecular disulfide bonds and have been released from calnexin [11].…”

Phylogenetic Analysis of Classical Swine Fever Virus Isolates from Peru

“…Several functions have been associated with E2 including virus attachment and entry of target cells and virulence (Risatti et al., 2007). E2 is also the most immunogenic of the CSFV glycoproteins, inducing neutralizing antibodies and protection against lethal CSFV challenge (Wensvoort, 1989; Konig et al., 1995; van Rijn et al., 1996). It is considered essential for CSFV replication, as virus mutants that contain either partial or complete deletions of the E2 gene have been shown to be non‐viable (van Gennip et al., 2002).…”

Plenary Lectures

“…Sub-unit vaccines have recently been obtained by the expression of the E2 major immunogen of classical swine fever virus in a baculovirus system (Konig et al, 1995;Van Rijn et al, 1999), in vaccinia or pseudorabies virus (E1) (Rumenaf et al, 1991;Van Zijl et al, 1991). The baculovirus expression system produced vaccines allowing the distinction between infected or vaccinated animals.…”