Classification and Molecular Biology

Ridpath, Julia F.

doi:10.1002/9780470344453.ch3

Cited by 12 publications

(5 citation statements)

References 138 publications

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“…The main difference between the cytopathic and noncytopathic biotypes of virus at the molecular level is an expression of nonstructural polypeptide NS3. Noncytopathic virus expresses the single polypeptide with a size of 125 KD (NS2-3 or p125), and cytopathic isolates have two proteins NS2-3 and NS3 [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

Virulent Properties of Russian Bovine Viral Diarrhea Virus Strains in Experimentally Infected Calves

Глотов¹,

Glotova²,

Koteneva³

et al. 2016

Scientifica

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The results of experimental study of three noncytopathic and two cytopathic bovine viral diarrhea virus (BVDV) strains isolated from cattle in the Siberian region and belonging to the type 1 (subtypes 1a, 1b, and 1d) have been presented. All investigated strains caused the development of infectious process in the seronegative 4–6-month-old calves after aerosol challenge with the dose of 6 log10 TCID50. The greatest virulence had noncytopathic strain and cytopathic strain related to the subtypes 1d and 1b, respectively. All strains in infected calves caused some signs of moderate acute respiratory disease and diarrhea: depression 3–5 days postinfection (p.i.), refusal to food, severe hyperthermia to 41.9°С, serous exudate discharges from the nasal cavity and eyes, transient diarrhea with blood, leukopenia (up to 2700 cells/mm3), and macroscopic changes in the respiratory organs and intestine. The infected animals recovered from 12 to 15 days p.i. and in 90% cases formed humoral immune response 25 days p.i. (antibody titers to BVDV: 1 : 4–1 : 16). Our results confirmed the presence of virulent BVDV1 strains and showed the need for researches on the molecular epidemiology of the disease, development of more effective diagnostic systems, and optimization of control programs with use of vaccines.

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Section: Discussionmentioning

confidence: 99%

Virulent Properties of Russian Bovine Viral Diarrhea Virus Strains in Experimentally Infected Calves

Глотов¹,

Glotova²,

Koteneva³

et al. 2016

Scientifica

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“…BVDV is an enveloped, single‐stranded RNA virus, and is the prototypic member of the genus Pestivirus within the family Flaviviridae. Currently recognized species within the Pestivirus genus include BVDV1, BVDV2, border disease virus, and classical swine fever virus (hog cholera virus) 1 . Strains of BVDV can exist as different biotypes, which are either cytopathic (CP) or noncytopathic (NCP).…”

Section: Virus Descriptionmentioning

confidence: 99%

Control of Bovine Viral Diarrhea Virus in Ruminants

Walz

Grooms

Passler

et al. 2010

Journal of Veterinary Internal Medicine

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“…Isolate V20 BVDV1/IndonesiaCJBms/0416164414/2016 iso late had two subgenotypes BVDV1a and BVDV1c. The presence of antigenic variation of E2 protein among BVD virus strains contributes to vaccination failure (Ridpath 2005). The structural region, E2 as hypervariation region used to in this study for the phylogenetic analysis and could be used to predict the rate of mutations in the evolution and virulence of the virus as well as factors that influence the pathogenesis of the virus in causing disease (Goens 2002; Dow et al 2015.…”

Section: Discussionmentioning

confidence: 99%

Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Irianingsih

Poermadjaja

Wuryastuti

et al. 2020

Indones. J. Biotechnol.

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The bovine viral diarrhea virus (BVDV) is a major viral pathogen in cattle worldwide. In Indonesia, diversity in subgenotypes of BVDV-1 has been observed, with the highest proportion of subgenotype -1a, followed by -1c, -1b, and -1d. So far, phylogenetic analysis of BVDV-1 is based on nucleotide sequences of the 5′ UTR and partial NS5B regions. Accuracy in identifying the subgenotype and antigenic type is critical for vaccine development and effective vaccination. The aim of this study was to determine genetic recombination of BVDV through phylogenetic analysis of five different regions (5′ UTR, NPro, E2, NS3, and NS5B) of BVDV in persistently infected dairy cattle. Five isolates were sequenced using next-generation sequencing, and data were analyzed with the CLC Genomic Workbench 9.0 and MEGA-X programs. Phylogenetic analysis based on the 5′ UTR (275 nt), NPro (504 nt), E2 (1,122 nt), NS3 (2,049 nt), and NS5B (2,157 nt) regions indicated that one BVDV isolate from Banyumas, Central Java, could be classified into different subgenotypes based on the E2 region (-1c), but the same subgenotype based on the other four regions (-1a), suggesting the presence of genetic recombination of the BVDV subgenotypes -1a and -1c in persistently infected dairy cattle.

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Classification and Molecular Biology

Cited by 12 publications

References 138 publications

Virulent Properties of Russian Bovine Viral Diarrhea Virus Strains in Experimentally Infected Calves

Virulent Properties of Russian Bovine Viral Diarrhea Virus Strains in Experimentally Infected Calves

Control of Bovine Viral Diarrhea Virus in Ruminants

Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

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