Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Irianingsih, Sri Handayani; Poermadjaja, Bagoes; Wuryastuti, Hastari; Wasito, R.

doi:10.22146/ijbiotech.54111

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…The sequences used in this study are accessible in GenBank with the accession numbers MK411754, MK411755, MK411756, MK411757, MK411762, MK411764, MK411765, MK411761 and MK411759 for BVDV-1a, MK 411760 for BVDV-1b and MK411751, MK411763, MK411753, MK411758 and MK411752 for the BVDC-1c genotypes. Analyses of fragments of 5 UTR (275 nt), Npro (504 nt) and NS3 (2049 nt) and NS5B (1038-2157 nt) of BVDV isolates from Banyumas supported the finding that the subgenotype BVDV-1a is circulating in that region, while a recombination of BVDV-1a with BVDV-1c was indicated in an analysis of 1093 nt of the E2 gene [48]. The BVDV-1 genotype was also detectable in the goat population in Central Java in a study on 288 nt of the 5 UTR gene [44].…”

Section: Genetic Diversitymentioning

confidence: 65%

“…Apart from culture-and protein-based tests, RNA-based tests have also been widely used to detect and characterise Indonesian isolates of BVDV, especially for genotyping [44][45][46]48,49].…”

Section: Isolation and Detectionmentioning

confidence: 99%

“…The five provinces with the most cattle should prioritise controlling BVDV in their cattle breeding farms (Figure 1). With the distinct characteristic that most breeding farms in Indonesia are small scale [47,48], the BVDV control programs in these farms are likely to be practical when co-organised by regional and provincial governments. However, a successful control strategy for BVDV in breeding farms would depend on provincial or even region-specific conditions [30,93].…”

Section: Control Of Bvdv In Breeding Farmsmentioning

confidence: 99%

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The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle

Nugroho

Silitonga²,

Reichel

et al. 2022

Pathogens

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This review aims to update the knowledge of the epidemiology of Bovine viral diarrhoea virus (BVDV) in Indonesia and Southeast Asia and provide a perspective on the control options for BVDV in the Indonesian cattle population in the future. Studies on BVDV in Indonesia, since its first report in that country, and the updated beef and dairy cattle industries are reviewed. In ten of 34 provinces, BVDV is endemic. The subgenotypes of BVDV-1a and BVDV-1c are predominant in Indonesian cattle. However, BVDV is currently not a priority disease to control in Indonesia. Cattle imports from Australia appear to be potentially the most significant source of transmission of BVDV into native cattle, but the control of BVDV conducted in the local quarantine facilities is currently not achieving the aim of controlling BVDV; thus, complementary measures are needed. With the small-scale nature of the vast majority of cattle breeding in the country, the control of BVDV in provinces in which cattle breeding is economically essential may need to be organised by regional and provincial governments. Gaps in our knowledge of BVDV are identified in this review, and strategies for the control of BVDV in Indonesia are discussed.

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Section: Genetic Diversitymentioning

confidence: 65%

Section: Isolation and Detectionmentioning

confidence: 99%

Section: Control Of Bvdv In Breeding Farmsmentioning

confidence: 99%

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The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle

Nugroho

Silitonga²,

Reichel

et al. 2022

Pathogens

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Novel genomic targets for proper subtyping of bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2

Mucellini,

Silva Júnior,

de Oliveira

et al. 2023

Virus Genes

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Genetic analyses of the structural protein E2 bovine viral diarrhea virus isolated from dairy cattle in Yogyakarta, Indonesia

Khan,

Wuryastuty,

Wibowo

et al. 2024

Vet World

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Background and Aim: Bovine viral diarrhea (BVD), a highly pathogenic ribonucleic acid (RNA) virus, causes devastating financial losses and reproductive deaths among dairy cattle in Yogyakarta and globally. This study aimed to identify point mutations within the E2 structural protein of the acquired BVD virus (BVDV) isolates using genetic analysis. Materials and Methods: The study period shows that we performed the research in 2023. We collected 118 serum samples from 2019 to 2023, among which only 10 BVDV positive were used and 108 were negative lacking the BVDV antigen. An anti-Erns monoclonal antibody-coated protein was used in indirect antigen capture enzyme-linked immunosorbent assay (I-ACE) to detect the BVD antigen present in positive BVDV serum specimens. In the initial step of the two-step reverse transcription polymerase chain reaction, the enzyme (superscript III reverse transcriptase) and the primer (random hexamer) were used to convert the RNA of the BVDV into complementary deoxyribonucleic acid (cDNA) during the process of reverse transcription. The final step involved the amplification of the E2 gene of the resultant BVDV cDNA through gene-specific primers (E2_fwd: 5′-TGGTGGCCTTATGAGAC-3′ and P7_rev: 5′-CCCATCATCACTATTTCACC-3′) and enzyme (platinum taq DNA polymerase high fidelity). For conducting Sanger sequencing, those 3 BVDV-1-positive isolates (about 2.6% of all isolates) were selected as a typical specimen for each site and year between 2019 and 2023 using a proportional computation. Therefore, only two BVDV isolates with complete genomes were chosen to perform their homological and genetic analysis based on the E2 gene by means of Blast and MEGA Version 11 in addition to the Bioedit 7.2.5 program. Results: By applying phylogenetic analysis relying on the E2 gene, a sum of 1011 nucleotides of the BVDV-1 isolates derived from each of the two BVDV-1 Indonesian isolates (n = 2) and its 23 reference BVDV strains were acquired from the National Center for Biotechnology Information (NCBI) database. The findings of the genetic analysis inside the phylogenetic tree revealed that the two BVDV Indonesian isolates were clustered into BVDV-1a subgenotype, while the reference BVDV strains were clustered into the five BVDV subgenotype, BVDV-1a (n = 6), BVDV-1b (n = 3), BVDV-1c (n = 11), BVDV-1m (n = 1), and BVDV-1n (n = 2). The branch exists in phylogenetic tree located before the division of our two BVDV isolates was divided into two branches with the same maximum bootstrap values of 99%, indicating a high degree of confidence, was seen. Next, we observed the branch near our study samples, which displayed the bootstrap value of 100, indicating that our 02 isolates were identical. In both isolates, V11 BVDV1/Indonesia/Yogyakarta/2023 and V16 BVDV1/Indonesia/ Yogyakarta/2023 with GenBank accession numbers PP836388 and PP836389, respectively, conserved D7E residues were mutated as well as cysteine changed/altered into serine (S) was identified at amino acid position 201. Conclusion: We identified two isolates of BVDV belonging to the BVDV-1a subgenotype. Our findings indicate that the conserved D7E residues of isolates V11 BVDV1/Indonesia/Yogyakarta/2023 and V16 BVDV1/Indonesia/Yogyakarta/2023 were altered. The Indonesian BVDV isolates exhibited a cysteine to serine mutation at amino acid position 201, leads to vaccination failure, range of animal’s host will increase, and diagnostic kit will not be effective. Keywords: bovine viral diarrhea virus, cysteine mutation, E2 protein, serine, V11 bovine viral diarrhea virus1, V16 bovine viral diarrhea virus1.

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Genetic recombination of bovine viral diarrhea virus subgenotype -1a and -1c in persistently infected dairy cattle

Cited by 4 publications

References 13 publications

The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle

The Epidemiology and Control of Bovine Viral Diarrhoea Virus in Tropical Indonesian Cattle

Novel genomic targets for proper subtyping of bovine viral diarrhea virus 1 (BVDV-1) and BVDV-2

Genetic analyses of the structural protein E2 bovine viral diarrhea virus isolated from dairy cattle in Yogyakarta, Indonesia

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