Classification of 27 Tumor-Associated Antigens by Histochemical Analysis of 36 Freshly Resected Lung Cancer Tissues

Abstract

In previous studies, we identified 29 tumor-associated antigens (TAAs) and isolated 488 human monoclonal antibodies (mAbs) that specifically bind to one of the 29 TAAs. In the present study, we performed histochemical analysis of 36 freshly resected lung cancer tissues by using 60 mAbs against 27 TAAs. Comparison of the staining patterns of tumor cells, bronchial epithelial cells, and normal pulmonary alveolus cells and interalveolar septum allowed us to determine the type and location of cells that express target molecules, as well as the degree of expression. The patterns were classified into 7 categories. While multiple Abs were used against certain TAAs, the differences observed among them should be derived from differences in the binding activity and/or the epitope. Thus, such data indicate the versatility of respective clones as anti-cancer drugs. Although the information obtained was limited to the lung and bronchial tube, bronchial epithelial cells represent normal growing cells, and therefore, the data are informative. The results indicate that 9 of the 27 TAAs are suitable targets for therapeutic Abs. These 9 Ags include EGFR, HER2, TfR, and integrin α6β4. Based on our findings, a pharmaceutical company has started to develop anti-cancer drugs by using Abs to TfR and integrin α6β4. HGFR, PTP-LAR, CD147, CDCP1, and integrin αvβ3 are also appropriate targets for therapeutic purposes.

A Novel High-Throughput Screening Method for a Human Multicentric Osteosarcoma-Specific Antibody and Biomarker Using a Phage Display-Derived Monoclonal Antibody

“…The human antibody library termed “Antibody Intended for Multiple Screening” (AIMS) and the related methodology suite have been well established by the Division of Antibody Project of the Fujita Health University, and have been used in several studies to develop cancer therapeutic antibodies [ 23 , 24 , 25 , 26 , 27 ]. The AIMS human antibody library comprises approximately 1 × 10 11 clone types obtained from human B-lymphocytes using the phage display method.…”

Σύγκριση Μοριακά Στοχευμένων Θεραπειών Πρώτης Γραμμής Στο Μεταστατικό Καρκίνωμα Του Νεφρού

“…Αναστολή των μορίων αυτών συνεπάγεται ενεργοποίηση των CTLs και προσβολή των κ.κ. (υπόψη ότι τα κατασταλτικά του ανοσοελέγχου μόρια, φυσιολογικά, εξασφαλίζουν την ανοχή του ΑΣ σε αυτοαντιγόνα και την πρόληψη αυτοάνοσων διαταραχών) (31,(38)(39)(40).…”